Application | Comment | Organism |
---|---|---|
food industry | the enzyme can alter glucocorticoid metabolism in the gut and thereby serves as potential probiotics for the management of androgen-dependent diseases | Bifidobacterium adolescentis |
Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia coli BL21(DE3)RIPL | Bifidobacterium adolescentis |
Crystallization (Comment) | Organism |
---|---|
sitting drop method, crystallization of the ternary complex of NADH and cortisol bound to the inactive S181A mutant, the structure is determined at 2.2 A for the apoform and 2.0 A for the binary complex with NADH | Bifidobacterium adolescentis |
Protein Variants | Comment | Organism |
---|---|---|
additional information | important role of the 38-residue extended N-terminus in protein stability as deletion of the first 21 residues effectively abolishes enzyme activity with a 1.45 °C shift in Tm | Bifidobacterium adolescentis |
S181A | inactive mutant enzyme | Bifidobacterium adolescentis |
S183A | the kcat/Km-value for cortisol is 56% compared to wild-type enzyme | Bifidobacterium adolescentis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02407 | - |
cortisol | pH 5.0, temperature not specified in the publication | Bifidobacterium adolescentis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
130500 | - |
gel filtration | Bifidobacterium adolescentis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
cortisol + NADH + H+ | Bifidobacterium adolescentis | - |
20beta-hydroxycortisol + NAD+ | - |
? | |
cortisol + NADH + H+ | Bifidobacterium adolescentis L2-32 | - |
20beta-hydroxycortisol + NAD+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bifidobacterium adolescentis | A7A7R9 | - |
- |
Bifidobacterium adolescentis L2-32 | A7A7R9 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Bifidobacterium adolescentis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
11-deoxycortisol + NADH + H+ | i.e. 4-pregnen-17,21-diol-3,20-dione, 66.03% compared to the activity with cortisol | Bifidobacterium adolescentis | ? + NAD+ | - |
? | |
11-deoxycortisol + NADH + H+ | i.e. 4-pregnen-17,21-diol-3,20-dione, 66.03% compared to the activity with cortisol | Bifidobacterium adolescentis L2-32 | ? + NAD+ | - |
? | |
corticosterone + NADH + H+ | i.e. 4-pregnen-11beta,21-diol-3,20-dione, 33.32% compared to the activity with cortisol | Bifidobacterium adolescentis | ? + NAD+ | - |
? | |
corticosterone + NADH + H+ | i.e. 4-pregnen-11beta,21-diol-3,20-dione, 33.32% compared to the activity with cortisol | Bifidobacterium adolescentis L2-32 | ? + NAD+ | - |
? | |
cortisol + NADH + H+ | - |
Bifidobacterium adolescentis | 20beta-hydroxycortisol + NAD+ | - |
? | |
cortisol + NADH + H+ | no activity with NADPH | Bifidobacterium adolescentis | 20beta-hydroxycortisol + NAD+ | - |
? | |
cortisol + NADH + H+ | - |
Bifidobacterium adolescentis L2-32 | 20beta-hydroxycortisol + NAD+ | - |
? | |
cortisol + NADH + H+ | no activity with NADPH | Bifidobacterium adolescentis L2-32 | 20beta-hydroxycortisol + NAD+ | - |
? | |
tetrahydrocortisol + NADH + H+ | i.e. 5beta-pregnan-3alpha,11beta,17,21-tetrol-20-one, 44.29% compared to the activity with cortisol | Bifidobacterium adolescentis | ? + NAD+ | - |
? | |
tetrahydrocortisol + NADH + H+ | i.e. 5beta-pregnan-3alpha,11beta,17,21-tetrol-20-one, 44.29% compared to the activity with cortisol | Bifidobacterium adolescentis L2-32 | ? + NAD+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 31700, SDS-PAGE | Bifidobacterium adolescentis |
Synonyms | Comment | Organism |
---|---|---|
20beta-HSDH | - |
Bifidobacterium adolescentis |
20beta-hydroxysteroid dehydrogenase | - |
Bifidobacterium adolescentis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
5 | - |
recombinant enzyme, reductive direction | Bifidobacterium adolescentis |
5.5 | - |
recombinant enzyme, oxidative direction | Bifidobacterium adolescentis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
56 | - |
transition temperature. Important role of the 38-residue extended N-terminus in protein stability as deletion of the first 21 residues effectively abolishes enzyme activity with a 1.45 °C shift in Tm | Bifidobacterium adolescentis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
11.1 | - |
cortisol | pH 5.0, temperature not specified in the publication | Bifidobacterium adolescentis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | no activity with NADPH | Bifidobacterium adolescentis |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme regulates cortisol side-chain cleavage by reducing the C-20 carboxyl group on cortisol | Bifidobacterium adolescentis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
461.1 | - |
cortisol | pH 5.0, temperature not specified in the publication | Bifidobacterium adolescentis |