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Literature summary for 1.1.1.146 extracted from

  • Maser, E.; Volker, B.; Friebertshauser, J.
    11 beta-Hydroxysteroid dehydrogenase type 1 from human liver: dimerization and enzyme cooperativity support its postulated role as glucocorticoid reductase (2002), Biochemistry, 41, 2459-2465.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
cloning of cDNA Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
glycyrrhetinic acid
-
Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.014
-
cortisone 37°C, pH 7.4, Hill coefficient of 1.6 Homo sapiens
0.02
-
dehydrocorticosterone 37°C, pH 7.4, Hill coefficient of 2.7 Homo sapiens
0.041
-
cortisol 37°C, pH 7.4, Hill coefficient of 1.0, no cooperative kinetic Homo sapiens
0.043
-
corticosterone 37°C, pH 7.4, Hill coefficient of 0.7 Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
endoplasmic reticulum
-
Homo sapiens 5783
-
membrane membrane bound Homo sapiens 16020
-
microsome
-
Homo sapiens
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32386
-
2 * 32386, deduced from nucleotide sequence Homo sapiens
34000
-
2 * 34000, SDS-PAGE Homo sapiens
67400
-
gel filtration Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cortisone + NADPH + H+ Homo sapiens primarly a reductase in vivo cortisol + NADP+
-
r

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
solubilized microsomes, octyl-Sepharose, Mono Q-Sepharose, Red Sepharose A Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
91.36
-
-
Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cortisone + NADPH + H+
-
Homo sapiens cortisol + NADP+
-
r
cortisone + NADPH + H+ primarly a reductase in vivo Homo sapiens cortisol + NADP+
-
r
dehydrocorticosterone + NADPH
-
Homo sapiens corticosterone + NADP+
-
r

Subunits

Subunits Comment Organism
dimer 2 * 34000, SDS-PAGE Homo sapiens
dimer 2 * 32386, deduced from nucleotide sequence Homo sapiens

Synonyms

Synonyms Comment Organism
11beta-hydroxysteroid dehydrogenase type 1
-
Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0000049
-
glycyrrhetinic acid 37°C, pH 9.0, inhibition of cortisol oxidation Homo sapiens
0.000014
-
glycyrrhetinic acid 37°C, pH 9.0, inhibition of cortisone reduction Homo sapiens