Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cd2+ | more potent inhibitor than Zn2+, reversible | Aspergillus parasiticus | |
Cu2+ | inactivation | Aspergillus parasiticus | |
KCl | half Vmax/Km at less than 0.1 M due to general ionic strength effect | Aspergillus parasiticus | |
KNO3 | half Vmax/Km at less than 0.1 M due to general ionic strength effect | Aspergillus parasiticus | |
NaCl | half Vmax/Km at less than 0.1 M due to general ionic strength effect | Aspergillus parasiticus | |
NADP+ | Ki: 0.014 mM | Aspergillus parasiticus | |
Zn2+ | reversible inhibition | Aspergillus parasiticus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.015 | - |
NADP+ | - |
Aspergillus parasiticus | |
100 | - |
D-mannitol | - |
Aspergillus parasiticus | |
190 | - |
D-arabinitol | - |
Aspergillus parasiticus | |
560 | - |
D-glucitol | - |
Aspergillus parasiticus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
32000 | - |
4 * 32000, SDS-PAGE | Aspergillus parasiticus |
140000 | - |
gel filtration | Aspergillus parasiticus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-mannitol + NADP+ | Aspergillus parasiticus | substrate must have a primary hydroxyl group at C1, an R hydroxyl at C2, an R hydroxyl at C3, an R hydroxyl at C4 and either an R or S or primary hydroxyl at C5 | D-fructose + NADPH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus parasiticus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
to homogeneity, chromatography techniques, affinity chromatography | Aspergillus parasiticus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
mycelium | - |
Aspergillus parasiticus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
130 | - |
- |
Aspergillus parasiticus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-deoxy-6-methyl-D-glucitol + NADP+ | poorer substrate than D-glucitol | Aspergillus parasiticus | 1-deoxy-6-methyl-D-glucose + NADPH | - |
? | |
1-deoxy-6-methyl-D-mannitol + NADP+ | poorer substrate than D-mannitol | Aspergillus parasiticus | 1-deoxy-6-methyl-D-fructose + NADPH | - |
? | |
1-deoxy-D-glucitol + NADP+ | poorer substrate than glucitol | Aspergillus parasiticus | 1-deoxy-D-glucose + NADPH | - |
? | |
1-deoxy-D-mannitol + NADP+ | poorer substrate than D-mannitol | Aspergillus parasiticus | 1-deoxy-D-fructose + NADPH | - |
? | |
D-arabinitol + NADP+ | substrate must have a primary hydroxyl group at C1, an R hydroxyl at C2, an R hydroxyl at C3, an R hydroxyl at C4 and either an R or S or primary hydroxyl at C5 | Aspergillus parasiticus | D-xylulose + NADPH + H+ | - |
? | |
D-glucitol + NADP+ | - |
Aspergillus parasiticus | D-glucose + NADPH + H+ | - |
r | |
D-mannitol + 3-acetylpyridine adenine dinucleotide phosphate | 7.5% of the activity compared to NADP+ | Aspergillus parasiticus | D-fructose + 3-acetylpyridine adenine dinucleotide phosphate(H) | - |
? | |
D-mannitol + NAD+ | less than 0.05% of the activity compared to NADP+ | Aspergillus parasiticus | D-fructose + NADH + H+ | - |
? | |
D-mannitol + NADP+ | substrate must have a primary hydroxyl group at C1, an R hydroxyl at C2, an R hydroxyl at C3, an R hydroxyl at C4 and either an R or S or primary hydroxyl at C5 | Aspergillus parasiticus | D-fructose + NADPH + H+ | - |
? | |
D-mannitol + thio-NADP+ | 30% of the activity compared to NADP+ | Aspergillus parasiticus | D-fructose + thio-NADPH | - |
? | |
D-sorbitol + NADP+ | - |
Aspergillus parasiticus | D-sorbose + NADPH + H+ | - |
? | |
L-sorbitol + NADP+ | substrate must have a primary hydroxyl group at C1, an R hydroxyl at C2, an R hydroxyl at C3, an R hydroxyl at C4 and either an R or S or primary hydroxyl at C5 | Aspergillus parasiticus | L-sorbose + NADPH | - |
r |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 32000, SDS-PAGE | Aspergillus parasiticus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9.5 | 95% of its maximal activity for 5 min | Aspergillus parasiticus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | specific for NADP+ | Aspergillus parasiticus |