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Literature summary for 1.1.1.133 extracted from

  • Law, A.; Stergioulis, A.; Halavaty, A.S.; Minasov, G.; Anderson, W.F.; Kuhn, M.L.
    Structure of the Bacillus anthracis dTDP-L-rhamnose-biosynthetic enzyme dTDP-4-dehydrorhamnose reductase (RfbD) (2017), Acta Crystallogr. Sect. F, 73, 644-650 .
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21(DE3) Bacillus anthracis

Crystallization (Commentary)

Crystallization (Comment) Organism
vapor diffusion sitting drop method, the 2.65 A resolution crystal structure of the enzyme in complex with NADP+ Bacillus anthracis

Metals/Ions

Metals/Ions Comment Organism Structure
additional information does not require Mg2+ for activity Bacillus anthracis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
dTDP-4-dehydro-beta-L-rhamnose + NADPH + H+ Bacillus anthracis enzyme in the dTDP-beta-L-rhamnose-biosynthetic pathway dTDP-beta-L-rhamnose + NADP+
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 ?

Organism

Organism UniProt Comment Textmining
Bacillus anthracis A0A6L7H7R4
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-

Purification (Commentary)

Purification (Comment) Organism
-
 Bacillus anthracis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dTDP-4-dehydro-beta-L-rhamnose + NADPH + H+
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 Bacillus anthracis dTDP-beta-L-rhamnose + NADP+
-
 ?
dTDP-4-dehydro-beta-L-rhamnose + NADPH + H+ enzyme in the dTDP-beta-L-rhamnose-biosynthetic pathway Bacillus anthracis dTDP-beta-L-rhamnose + NADP+
-
 ?

Subunits

Subunits Comment Organism
monomer
-
 Bacillus anthracis

Synonyms

Synonyms Comment Organism
dTDP-4-dehydrorhamnose reductase
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 Bacillus anthracis
RfbD
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 Bacillus anthracis

Cofactor

Cofactor Comment Organism Structure
NADPH based on our structural comparison of the enzyme (RfbD) with its homologs, it is found that RfbD is likely to utilize either NAD(H) or NADP(H) as a coenzyme Bacillus anthracis

General Information

General Information Comment Organism
drug target the dTDP-beta-L-rhamnose-biosynthetic pathway is absent in humans, which make them ideal targets for therapeutic development to combat pathogens (e.g. Bacillus anthracis, the causative agent of the deadly disease Anthrax) Bacillus anthracis
metabolism enzyme in the dTDP-beta-L-rhamnose-biosynthetic pathway Bacillus anthracis