Literature summary for 1.1.1.100 extracted from

Zhang, Y.; Ning, F.; Li, X.; Teng, M.
Structural insights into cofactor recognition of yeast mitochondria 3-oxoacyl-ACP reductase OAR1 (2013), IUBMB Life, 65, 154-162.

Search for more literature for 1.1.1.100

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Saccharomyces cerevisiae

Crystallization (Commentary)

Crystallization (Comment)	Organism
apo-form complexed with NADPH, vapor diffusion method, using 10% (w/v) polyethylene glycol 4000, 10% (v/v) 2-propanol, 0.1 M HEPES pH 7.0 and 30% (v/v) jeffamine ED2001, 0.1 M HEPES pH 7.5	Saccharomyces cerevisiae

Protein Variants

Protein Variants	Comment	Organism
R14A	inactive	Saccharomyces cerevisiae
S36A	the mutant shows reduced activity compared to the wild type enzyme	Saccharomyces cerevisiae
S40A	the mutant shows reduced activity compared to the wild type enzyme	Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0205	-	NADPH	wild type enzyme, at pH 8.0 and 25°C	Saccharomyces cerevisiae
0.0264	-	NADPH	mutant enzyme S36A, at pH 8.0 and 25°C	Saccharomyces cerevisiae
0.0571	-	NADPH	mutant enzyme S40A, at pH 8.0 and 25°C	Saccharomyces cerevisiae
0.8158	-	acetoacetyl-CoA	wild type enzyme, at pH 8.0 and 25°C	Saccharomyces cerevisiae
0.8871	-	acetoacetyl-CoA	mutant enzyme S36A, at pH 8.0 and 25°C	Saccharomyces cerevisiae
0.9913	-	acetoacetyl-CoA	mutant enzyme S40A, at pH 8.0 and 25°C	Saccharomyces cerevisiae

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
mitochondrion	-	Saccharomyces cerevisiae	5739	-

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
a 3-oxoacyl-[acyl-carrier protein] + NADPH + H+	Saccharomyces cerevisiae	-	a (3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+	-	?

Organism

Organism	UniProt	Comment	Textmining
Saccharomyces cerevisiae	P35731	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-NTA resin column chromatography and Superdex 200 gel filtration	Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
a 3-oxoacyl-[acyl-carrier protein] + NADPH + H+	-	Saccharomyces cerevisiae	a (3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+	-	?
acetoacetyl-CoA + NADPH + H+	-	Saccharomyces cerevisiae	?	-	?

Subunits

Subunits	Comment	Organism
homodimer	x-ray crystallography	Saccharomyces cerevisiae

Synonyms

Synonyms	Comment	Organism
3-oxoacyl-(acyl-carrier-protein) reductase	-	Saccharomyces cerevisiae
3-oxoacyl-ACP reductase	-	Saccharomyces cerevisiae
FabG	-	Saccharomyces cerevisiae
OAR1	-	Saccharomyces cerevisiae

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
91.4	-	NADPH	mutant enzyme S40A, at pH 8.0 and 25°C	Saccharomyces cerevisiae
91.4	-	acetoacetyl-CoA	mutant enzyme S40A, at pH 8.0 and 25°C	Saccharomyces cerevisiae
108.2	-	NADPH	mutant enzyme S36A, at pH 8.0 and 25°C	Saccharomyces cerevisiae
108.2	-	acetoacetyl-CoA	mutant enzyme S36A, at pH 8.0 and 25°C	Saccharomyces cerevisiae
117.5	-	NADPH	wild type enzyme, at pH 8.0 and 25°C	Saccharomyces cerevisiae
117.5	-	acetoacetyl-CoA	wild type enzyme, at pH 8.0 and 25°C	Saccharomyces cerevisiae

Cofactor

Cofactor	Comment	Organism	Structure
NADPH	-	Saccharomyces cerevisiae

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Release 2023.1 (January 2023)