Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | affinity of acyl-carrier protein for FabG is increased by 3fold in the presence of 1 M urea | Plasmodium falciparum |
Cloned (Comment) | Organism |
---|---|
into pET-28a vector and expressed in Escherichia coli BL21 (DE3) codon plus | Plasmodium falciparum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Guanidinium chloride | FabG is fully unfolded at 4 M, approximately 90% of the enzyme activity can be recovered on dialyzing the denaturant. In presence of NADPH, there is no stabilization of FabG in case of equilibrium unfolding with guanidinium chloride | Plasmodium falciparum | |
Urea | FabG is fully unfolded at 6 M, approximately 90% of the enzyme activity can be recovered on dialyzing the denaturant. Two states in the reversible unfolding process of FabG in presence of NADPH, one is an activity-enhanced state and the other, an inactive state in case of equilibrium unfolding with urea | Plasmodium falciparum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0412 | - |
NADPH | in the presence of 1.5 M urea, pH 7.0, 25°C | Plasmodium falciparum | |
0.0434 | - |
NADPH | in the presence of 1 M urea, pH 7.0, 25°C | Plasmodium falciparum | |
0.0461 | - |
NADPH | without urea, pH 7.0, 25°C | Plasmodium falciparum | |
0.4553 | - |
NADPH | in the presence of 4 M urea, pH 7.0, 25°C | Plasmodium falciparum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Plasmodium falciparum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Plasmodium falciparum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-oxoacyl-[acyl-carrier protein] + NADPH | - |
Plasmodium falciparum | (3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+ | - |
? | |
acetoacetyl-CoA + NADPH | - |
Plasmodium falciparum | 3-hydroxybutyryl-CoA + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | cross-linking of native and up to 1 M urea-treated FabG | Plasmodium falciparum |
Synonyms | Comment | Organism |
---|---|---|
beta-ketoacyl-ACP reductase | - |
Plasmodium falciparum |
FabG | - |
Plasmodium falciparum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
148.6 | - |
NADPH | in the presence of 4 M urea, pH 7.0, 25°C | Plasmodium falciparum | |
187.3 | - |
NADPH | in the presence of 1.5 M urea, pH 7.0, 25°C | Plasmodium falciparum | |
250 | - |
NADPH | without urea, pH 7.0, 25°C | Plasmodium falciparum | |
344.7 | - |
NADPH | in the presence of 1 M urea, pH 7.0, 25°C | Plasmodium falciparum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Plasmodium falciparum |