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Literature summary for 1.1.1.100 extracted from

  • Li, B.H.; Ma, X.F.; Wu, X.D.; Tian, W.X.
    Inhibitory activity of chlorogenic acid on enzymes involved in the fatty acid synthesis in animals and bacteria (2006), IUBMB Life, 58, 39-46.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene fabG, expression of N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3) Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
chlorogenic acid IC50: 0.881-0.948 mM Escherichia coli
NADP+ competitive product inhibition Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
gene fabG
-

Purification (Commentary)

Purification (Comment) Organism
recombinant N-terminally His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetoacetyl-CoA + NADPH
-
Escherichia coli 3-hydroxybutyryl-CoA + NADP+
-
?

Synonyms

Synonyms Comment Organism
beta-ketoacyl-ACP reductase
-
Escherichia coli
FabG
-
Escherichia coli

Temperature Optimum [┬░C]

Temperature Optimum [┬░C] Temperature Optimum Maximum [┬░C] Comment Organism
37
-
assay at Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
assay at Escherichia coli

Cofactor

Cofactor Comment Organism Structure
NADPH
-
Escherichia coli

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
additional information
-
additional information inhibition kinetic analysis Escherichia coli

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.881 0.948 IC50: 0.881-0.948 mM Escherichia coli chlorogenic acid