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Literature summary for 1.1.1.100 extracted from

  • Karmodiya, K.; Surolia, N.
    Analyses of co-operative transitions in Plasmodium falciparum beta-ketoacyl acyl carrier protein reductase upon co-factor and acyl carrier protein binding (2006), FEBS J., 273, 4093-4103.
    View publication on PubMed

Application

Application Comment Organism
drug development the enzyme has no isoforms and thus is a good target for inhibitor design Plasmodium falciparum

Cloned(Commentary)

Cloned (Comment) Organism
gene fabG, DNA and amino acid sequence determination and analysis, expression of C-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3) Plasmodium falciparum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information cooperativity kinetics, binding constants of substrates and cofactors Plasmodium falciparum
0.043
-
NADPH pH 6.8, 22┬░C, recombinant enzyme Plasmodium falciparum
0.43
-
acetoacetyl-CoA pH 6.8, 22┬░C, recombinant enzyme Plasmodium falciparum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3-oxoacyl-[acyl-carrier protein] + NADPH Plasmodium falciparum
-
3-hydroxyacyl-[acyl-carrier protein] + NADP+
-
r

Organism

Organism UniProt Comment Textmining
Plasmodium falciparum
-
gene fabG, no isozymes
-

Purification (Commentary)

Purification (Comment) Organism
recombinant C-terminally His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Plasmodium falciparum

Reaction

Reaction Comment Organism Reaction ID
a (3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+ = a 3-oxoacyl-[acyl-carrier protein] + NADPH + H+ cofactor and substrate binding kinetics, allosteric regulation mechanism, overview Plasmodium falciparum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [┬Ámol/min/mg] Specific Activity Maximum [┬Ámol/min/mg] Comment Organism
59.8
-
purified recombinant enzyme Plasmodium falciparum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-oxoacyl-[acyl-carrier protein] + NADPH
-
Plasmodium falciparum 3-hydroxyacyl-[acyl-carrier protein] + NADP+
-
r
3-oxoacyl-[acyl-carrier protein] + NADPH cooperative transitions in the enzyme due to cofactor and [acyl-carrier-protein] binding Plasmodium falciparum 3-hydroxyacyl-[acyl-carrier protein] + NADP+
-
r
acetoacetyl-CoA + NADPH
-
Plasmodium falciparum 3-hydroxybutyryl-CoA + NADP+
-
r

Synonyms

Synonyms Comment Organism
beta-ketoacyl acyl carrier protein reductase
-
Plasmodium falciparum
FabG
-
Plasmodium falciparum

Temperature Optimum [┬░C]

Temperature Optimum [┬░C] Temperature Optimum Maximum [┬░C] Comment Organism
22
-
assay at room temperature Plasmodium falciparum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
259
-
NADPH pH 6.8, 22┬░C, recombinant enzyme Plasmodium falciparum
259
-
acetoacetyl-CoA pH 6.8, 22┬░C, recombinant enzyme Plasmodium falciparum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8
-
assay at Plasmodium falciparum

Cofactor

Cofactor Comment Organism Structure
NADP+ cooperative transitions in the enzyme due to cofactor and [acyl-carrier-protein] binding, cooperative cofactor binding Plasmodium falciparum
NADPH cooperative transitions in the enzyme due to cofactor and [acyl-carrier-protein] binding, cooperative allosteric cofactor binding in presence of [acyl-carrier-protein] Plasmodium falciparum