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Literature summary for 1.1.1.100 extracted from

  • Nomura, C.T.; Taguchi, K.; Gan, Z.; Kuwabara, K.; Tanaka, T.; Takase, K.; Doi, Y.
    Expression of 3-ketoacyl-acyl carrier protein reductase (fabG) genes enhances production of polyhydroxyalkanoate copolymer from glucose in recombinant Escherichia coli JM109 (2005), Appl. Environ. Microbiol., 71, 4297-4306.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene fabG, overexpression of His-tagged enzyme in strain BL21(DE3) Escherichia coli
gene fabG, overexpression of His-tagged enzyme in strain BL21(DE3), recombinant expression in Escherichia coli strain JM109 leads to enhanced production of polyhydroxyalkanoate, PHA, copolymer from glucose, PHA composition, overview Pseudomonas sp.

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
gene fabG
-
Pseudomonas sp. Q4AE87 strain 61-3, non-pathogenic strain from soil, gene fabG
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from strain BL21(DE3) by nickel affinity chromatography Escherichia coli
recombinant His-tagged enzyme from strain BL21(DE3) by nickel affinity chromatography Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-oxodecanoyl-CoA + NADPH
-
Escherichia coli (R)-3-hydroxydecanoyl-CoA + NADP+
-
?
3-oxodecanoyl-CoA + NADPH
-
Pseudomonas sp. (R)-3-hydroxydecanoyl-CoA + NADP+
-
?
3-oxododecanoyl-CoA + NADPH
-
Escherichia coli (R)-3-hydroxydodecanoyl-CoA + NADP+
-
?
3-oxododecanoyl-CoA + NADPH
-
Pseudomonas sp. (R)-3-hydroxydodecanoyl-CoA + NADP+
-
?
3-oxohexanoyl-CoA + NADPH
-
Escherichia coli (R)-3-hydroxyhexanoyl-CoA + NADP+
-
?
3-oxohexanoyl-CoA + NADPH
-
Pseudomonas sp. (R)-3-hydroxyhexanoyl-CoA + NADP+
-
?
3-oxooctanoyl-CoA + NADPH
-
Escherichia coli (R)-3-hydroxyoctanoyl-CoA + NADP+
-
?
3-oxooctanoyl-CoA + NADPH
-
Pseudomonas sp. (R)-3-hydroxyoctanoyl-CoA + NADP+
-
?
acetoacetyl-CoA + NADPH
-
Escherichia coli 3-hydroxybutyryl-CoA + NADP+
-
?
acetoacetyl-CoA + NADPH
-
Pseudomonas sp. 3-hydroxybutyryl-CoA + NADP+
-
?
additional information FabG substrate specificity overview Escherichia coli ?
-
?
additional information FabG substrate specificity overview Pseudomonas sp. ?
-
?

Synonyms

Synonyms Comment Organism
3-ketoacyl-acyl carrier protein reductase
-
Escherichia coli
3-ketoacyl-acyl carrier protein reductase
-
Pseudomonas sp.
FabG
-
Escherichia coli
FabG
-
Pseudomonas sp.

Temperature Optimum [┬░C]

Temperature Optimum [┬░C] Temperature Optimum Maximum [┬░C] Comment Organism
22
-
assay at room temperature Escherichia coli
22
-
assay at room temperature Pseudomonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Escherichia coli
8
-
assay at Pseudomonas sp.

Cofactor

Cofactor Comment Organism Structure
NADPH
-
Escherichia coli
NADPH
-
Pseudomonas sp.