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Literature summary for 1.1.1.10 extracted from

  • Witteveen, C.F.B.; Weber, F.; Busink, R.; Visser, J.
    Isolation and characterization of two xylitol dehydrogenases from Aspergillus niger (1994), Microbiology, 140, 1679-1685.
No PubMed abstract available

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.03
-
NADPH
-
Aspergillus niger
0.13
-
NADP+
-
Aspergillus niger
17
-
L-xylulose
-
Aspergillus niger
925
-
xylitol
-
Aspergillus niger

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32000
-
8 * 32000, SDS-PAGE Aspergillus niger
250000
-
gel filtration Aspergillus niger

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-xylulose + NADPH + H+ Aspergillus niger
-
L-xylitol + NADP+
-
r

Organism

Organism UniProt Comment Textmining
Aspergillus niger
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Aspergillus niger

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
33
-
xylitol oxidation, NADP+ Aspergillus niger
200
-
L-xylulose reduction, NADPH Aspergillus niger

Storage Stability

Storage Stability Organism
4°C, Tris-HCl 20 mM, pH 7.0, at leat several weeks Aspergillus niger

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-sorbitol + NADP+
-
Aspergillus niger ?
-
?
L-xylulose + NADPH + H+
-
Aspergillus niger L-xylitol + NADP+
-
r
additional information minimal reductive activity also with D-xylulose, D-erythrose and dihydroxyacetone Aspergillus niger ?
-
?
xylitol + NADP+
-
Aspergillus niger L-xylulose + NADPH + H+
-
r

Subunits

Subunits Comment Organism
octamer 8 * 32000, SDS-PAGE Aspergillus niger

Synonyms

Synonyms Comment Organism
More xylitol:NAD+ 2-oxidoreductase, EC 1.1.1.9, is termed as xylitol dehydrogenase Aspergillus niger

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
reduction of L-xylulose Aspergillus niger
10 11 oxidation of xylitol Aspergillus niger

pH Range

pH Minimum pH Maximum Comment Organism
5 8.5 reduction of L-xylulose Aspergillus niger
8 11.5 oxidation of xylitol Aspergillus niger

Cofactor

Cofactor Comment Organism Structure
additional information determination of Ki of NADP+ and NADPH in competition with each other Aspergillus niger
NAD+ no activity with Aspergillus niger
NADP+
-
Aspergillus niger
NADPH preference for Aspergillus niger