Cloned (Comment) | Organism |
---|---|
gene ADH3, subcloning in Escherichia coli strain DH5alpha, recombinant expression of His6-tagged enzyme in Echerichia coli strain BL21 (DE3), complementation of the HpADH3 mutant by an HpADH3 expression cassette fused to a strong constitutive promoter, the resulting strain produced a significantly increased amount of ethanol compared to the wild-type strain in a glucose medium, while in a xylose medium, the ethanol production is dramatically reduced in an HpADH3 overproduction strain compared to that in the wild-type strain, semi-quantitative RT-PCR analysis | Ogataea angusta |
Protein Variants | Comment | Organism |
---|---|---|
additional information | generation of the ADH3 deletion mutant, complementation of the HpADH3 mutant by an HpADH3 expression cassette fused to a strong constitutive promoter, the resulting strain produced a significantly increased amount of ethanol compared to the wild-type strain in a glucose medium, while in a xylose medium, the ethanol production is dramatically reduced in an HpADH3 overproduction strain compared to that in the wild-type strain, phenotype analysis of DELTAHpADH3 and DELTAHpADH1/DELTAHpADH3 mutants, overview | Ogataea angusta |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.45 | - |
ethanol | pH 9.0, 22°C, recombinant enzyme | Ogataea angusta | |
0.67 | - |
acetaldehyde | pH 6.0, 22°C, recombinant enzyme | Ogataea angusta |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | HpADH3 | Ogataea angusta | 5739 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetaldehyde + NADH + H+ | Ogataea angusta | - |
ethanol + NAD+ | - |
r | |
acetaldehyde + NADH + H+ | Ogataea angusta DL-1 | - |
ethanol + NAD+ | - |
r | |
ethanol + NAD+ | Ogataea angusta | - |
acetaldehyde + NADH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ogataea angusta | H9ZGN0 | i.e. Pichia angusta, HpADH3 is not the major ADH in strain DL-1, gene ADH3 | - |
Ogataea angusta DL-1 | H9ZGN0 | i.e. Pichia angusta, HpADH3 is not the major ADH in strain DL-1, gene ADH3 | - |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged enzyme from Echerichia coli strain BL21 (DE3) by nickel affinity chromatography | Ogataea angusta |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,2-butanediol + NAD+ | low activity | Ogataea angusta | ? | - |
? | |
1,2-butanediol + NAD+ | low activity | Ogataea angusta DL-1 | ? | - |
? | |
1-butanol + NAD+ | - |
Ogataea angusta | butanal + NADH + H+ | - |
r | |
1-butanol + NAD+ | - |
Ogataea angusta DL-1 | butanal + NADH + H+ | - |
r | |
1-octanol + NAD+ | - |
Ogataea angusta | octanal + NADH + H+ | - |
r | |
1-pentanol + NAD+ | - |
Ogataea angusta | pentanal + NADH + H+ | - |
r | |
1-propanol + NAD+ | - |
Ogataea angusta | propanaldehyde + NADH + H+ | - |
r | |
2-methoxyethanol + NAD+ | - |
Ogataea angusta | 2-methoxyacetaldehyde + NADH + H+ | - |
r | |
2-octanol + NAD+ | low activity | Ogataea angusta | 2-octanone + NADH + H+ | - |
r | |
2-pentanol + NAD+ | low activity | Ogataea angusta | 2-pentanone + NADH + H+ | - |
r | |
2-propanol + NAD+ | - |
Ogataea angusta | 2-propanone + NADH + H+ | - |
r | |
acetaldehyde + NADH + H+ | - |
Ogataea angusta | ethanol + NAD+ | - |
r | |
acetaldehyde + NADH + H+ | best substrate | Ogataea angusta | ethanol + NAD+ | - |
r | |
acetaldehyde + NADH + H+ | - |
Ogataea angusta DL-1 | ethanol + NAD+ | - |
r | |
acetaldehyde + NADH + H+ | best substrate | Ogataea angusta DL-1 | ethanol + NAD+ | - |
r | |
ethanol + NAD+ | - |
Ogataea angusta | acetaldehyde + NADH + H+ | - |
r | |
additional information | methanol, formaldehyde, and acetone are no substrates for HpADH3 | Ogataea angusta | ? | - |
? | |
additional information | methanol, formaldehyde, and acetone are no substrates for HpADH3 | Ogataea angusta DL-1 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
alcohol dehydrogenase 3 | - |
Ogataea angusta |
HpADH3 | - |
Ogataea angusta |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
assay at room temperature | Ogataea angusta |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3000 | - |
ethanol | pH 9.0, 22°C, recombinant enzyme | Ogataea angusta | |
3500 | - |
acetaldehyde | pH 6.0, 22°C, recombinant enzyme | Ogataea angusta |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
aldehyde reduction | Ogataea angusta |
9 | - |
alcohol oxidation | Ogataea angusta |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Ogataea angusta | |
NADH | - |
Ogataea angusta |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
6667 | - |
ethanol | pH 9.0, 22°C, recombinant enzyme | Ogataea angusta | |
70000 | - |
acetaldehyde | pH 6.0, 22°C, recombinant enzyme | Ogataea angusta |