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Literature summary for 1.1.1.1 extracted from

  • Cao, Y.; Liao, L.; Xu, X.W.; Oren, A.; Wang, C.; Zhu, X.F.; Wu, M.
    Characterization of alcohol dehydrogenase from the haloalkaliphilic archaeon Natronomonas pharaonis (2008), Extremophiles, 12, 471-476.
    View publication on PubMed
Search for more literature for 1.1.1.1

Cloned(Commentary)

Cloned (Comment) Organism
gene encoding for ADH of the haloalkaliphilic archaeon Natronomonas pharaonis, which has a 1,068-bp open reading frame that encodes a protein of 355 amino acids, is cloned into the pET28b vector and is expressed in Escherichia coli Natronomonas pharaonis

Inhibitors

Inhibitors Comment Organism Structure
Zn2+
-
 Natronomonas pharaonis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.013
-
 acetaldehyde 70°C, pH 9.0, 50 mM Tris-HCl, 4 M NaCl Natronomonas pharaonis
0.022
-
 ethanol 70°C, pH 9.0, 50 mM Tris-HCl, 4 M NaCl Natronomonas pharaonis
0.059
-
 acetaldehyde 40°C, pH 9.0, 50 mM Tris-HCl, 4 M NaCl Natronomonas pharaonis
0.34
-
 ethanol 40°C, pH 9.0, 50 mM Tris-HCl, 4 M NaCl Natronomonas pharaonis

Metals/Ions

Metals/Ions Comment Organism Structure
KCl most active at 5 M NaCl or 4 M KCl Natronomonas pharaonis
NaCl most active at 5 M NaCl or 4 M KCl Natronomonas pharaonis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
41300
-
 x * 41300, SDS-PAGE Natronomonas pharaonis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ethanol + NAD+ Natronomonas pharaonis
-
 acetaldehyde + NADH
-
 r

Organism

Organism UniProt Comment Textmining
Natronomonas pharaonis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Natronomonas pharaonis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-butanol + NAD+ 15.7% of the activity with ethanol Natronomonas pharaonis butanal + NADH + H+
-
 ?
ethanol + NAD+
-
 Natronomonas pharaonis acetaldehyde + NADH
-
 r
ethanol + NAD+ the enzyme is highly specific for ethanol with NAD+ as the coenzyme Natronomonas pharaonis acetaldehyde + NADH + H+
-
 r

Subunits

Subunits Comment Organism
? x * 41300, SDS-PAGE Natronomonas pharaonis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
-
 Natronomonas pharaonis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
50 80 50°C: 45% of maximal activity, 80°C: about 60% of maximal activity Natronomonas pharaonis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 1 h, stable Natronomonas pharaonis
50
-
 1 h, 15% loss of activity Natronomonas pharaonis
60
-
 1 h, 20% loss of activity Natronomonas pharaonis
70
-
 1 h, 40% loss of activity Natronomonas pharaonis
80
-
 1 h, 50% loss of activity Natronomonas pharaonis
90
-
 1 h, 95% loss of activity Natronomonas pharaonis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.3
-
 acetaldehyde 40°C, pH 9.0, 50 mM Tris-HCl, 4 M NaCl Natronomonas pharaonis
0.4
-
 ethanol 40°C, pH 9.0, 50 mM Tris-HCl, 4 M NaCl Natronomonas pharaonis
5.4
-
 acetaldehyde 70°C, pH 9.0, 50 mM Tris-HCl, 4 M NaCl Natronomonas pharaonis
8.2
-
 ethanol 70°C, pH 9.0, 50 mM Tris-HCl, 4 M NaCl Natronomonas pharaonis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
 Natronomonas pharaonis

pH Range

pH Minimum pH Maximum Comment Organism
8 12 pH 8.0: about 70% of maximal activity, pH 12: about 60% of maximal activity Natronomonas pharaonis

pH Stability

pH Stability pH Stability Maximum Comment Organism
1 2 30°C, 1 h, 15% loss of activity Natronomonas pharaonis
4
-
 30°C, 1 h, 30% loss of activity Natronomonas pharaonis
5
-
 30°C, 1 h, 20% loss of activity Natronomonas pharaonis
6 11 30°C, 1 h, less than 10% loss of activity Natronomonas pharaonis