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Literature summary for 1.1.1.1 extracted from

  • Chou, C.F.; Lai, C.L.; Chang, Y.C.; Duester, G.; Yin, S.J.
    Kinetic mechanism of human class IV alcohol dehydrogenase functioning as retinol dehydrogenase (2002), J. Biol. Chem., 277, 25209-25216.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of ADH4 in Escherichia coli Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
all-trans-retinal product inhibition Homo sapiens
all-trans-retinoic acid weak feedback inhibition Homo sapiens
Isobutyramide substrate inhibition, competitive against retinol, noncompetitive against NADH Homo sapiens
trifluoroethanol competitive against retinol, noncompetitive against NAD+ Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information steady-state kinetics, kinetic mechanism Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
all-trans-retinol + NAD+ Homo sapiens ADH4 might be involved in biosynthesis of retinoic acid all-trans-retinal + NADH
-
r
isobutyramide + NAD+ Homo sapiens
-
?
-
r

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
class IV alcohol dehydrogenase
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme from Escherichia coli by DEAE ion exchange, 5'-AMP-resin affinity, and Mono Q ion exchange chromatography Homo sapiens

Reaction

Reaction Comment Organism Reaction ID
a primary alcohol + NAD+ = an aldehyde + NADH + H+ class IV alcohol dehydrogenase also functions as retinol dehydrogenase, reaction and kinetic mechanism: asymmetric rapid equilibrium random mechanism with 2 dead-end ternary complexes fro retinol oxidation and a rapid equilibrium ordered mechanism with one dead-end ternary complex for retinal reduction, a unique mechanistic form fro zinc-containing ADH in the medium chain dehydrogenase/reductase superfamily of enzymes Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
all-trans-retinol + NAD+
-
Homo sapiens all-trans-retinal + NADH
-
r
all-trans-retinol + NAD+ ADH4 might be involved in biosynthesis of retinoic acid Homo sapiens all-trans-retinal + NADH
-
r
isobutyramide + NAD+
-
Homo sapiens ?
-
r

Synonyms

Synonyms Comment Organism
ADH4
-
Homo sapiens

Temperature Optimum [┬░C]

Temperature Optimum [┬░C] Temperature Optimum Maximum [┬░C] Comment Organism
25
-
assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Homo sapiens

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Homo sapiens
NADH
-
Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
additional information
-
additional information
-
Homo sapiens