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Literature summary for 1.1.1.1 extracted from

  • Vanni, A.; Anfossi, L.; Pessione, E.; Giovannoli, C.
    Catalytic and spectroscopic characterisation of a copper-substituted alcohol dehydrogenase from yeast (2002), Int. J. Biol. Macromol., 30, 41-45.
    View publication on PubMed
Search for more literature for 1.1.1.1

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
12
-
 ethanol pH 7.5, 25°C, Co-ADH Saccharomyces cerevisiae
39
-
 ethanol pH 7.5, 25°C, Cu-ADH Saccharomyces cerevisiae
53
-
 ethanol pH 7.5, 25°C, native Zn-ADH Saccharomyces cerevisiae

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ construction of an active metal-substituted mutant by substituting Zn2+ for Cu2+ or Co2+, which maintain the same configuration as the native zinc ion, but possessing a wider pH range and a lower activity and substrate affinity than the wild-type enzyme, overview Saccharomyces cerevisiae
Cu2+ construction of an active metal-substituted mutant by substituting Zn2+ for Cu2+ or Co2+, which maintain the same configuration as the native zinc ion, but possessing a wider pH range and a lower activity and substrate affinity than the wild-type enzyme, overview Saccharomyces cerevisiae
Zn2+ native enzyme contains catalytic zinc ions Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ethanol + NAD+ Saccharomyces cerevisiae rate-limiting step of the alcoholic fermentation acetaldehyde + NADH
-
 ?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Renatured (Commentary)

Renatured (Comment) Organism
Zn2+ withdrawal by inactivation with Chelex 100, reactivation of the apoenzyme by addition of CuSO4, 1 h at 25°C, pH 7.6 Saccharomyces cerevisiae

Source Tissue

Source Tissue Comment Organism Textmining
commercial preparation lyophilized Saccharomyces cerevisiae
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-propanol + NAD+
-
 Saccharomyces cerevisiae acetone + NADH
-
 ?
ethanol + NAD+
-
 Saccharomyces cerevisiae acetaldehyde + NADH
-
 ?
ethanol + NAD+ rate-limiting step of the alcoholic fermentation Saccharomyces cerevisiae acetaldehyde + NADH
-
 ?
n-butanol + NAD+
-
 Saccharomyces cerevisiae butyraldehyde + NADH
-
 ?
n-propanol + NAD+
-
 Saccharomyces cerevisiae propanal + NADH
-
 ?

Synonyms

Synonyms Comment Organism
Y-ADH
-
 Saccharomyces cerevisiae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
 Zn-ADH and Co-ADH Saccharomyces cerevisiae
40
-
 Cu-ADH Saccharomyces cerevisiae

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
15 50
-
 Saccharomyces cerevisiae

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
56
-
 native Zn-ADH enzyme Saccharomyces cerevisiae
69
-
 Cu-ADH enzyme Saccharomyces cerevisiae
70
-
 above, Co-ADH enzyme Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 Zn-ADH, Co-ADH, and Cu-ADH Saccharomyces cerevisiae

pH Range

pH Minimum pH Maximum Comment Organism
6 9
-
 Saccharomyces cerevisiae

pH Stability

pH Stability pH Stability Maximum Comment Organism
6.5 9 native Zn-ADH enzyme Saccharomyces cerevisiae
6.5
-
 below, Cu-ADH and Co-ADH Saccharomyces cerevisiae