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sucrose + alpha-D-glucan
?
sucrose + alpha-D-glucan
alternating-1,6-1,3-alpha-D-glucan
sucrose + alpha-D-glucopyranosyl-(1,4)-L-glucose
?
sucrose + butyl alpha-D-glucopyranoside
?
-
-
-
-
?
sucrose + cellobiose
oligosaccharides
sucrose + D-tagatose
alpha-D-glucopyranosyl-(1,1)-beta-D-tagatopyranose
sucrose + isomaltose
oligoalternan
sucrose + L-glucose
alpha-D-glucopyranosyl-(1,6)-alpha-D-glucopyranosyl-(1,4)-L-glucose
sucrose + leucrose
?
-
regioselectivity of alternansucrase differs from dextransucrase. Alternansucrase shows greater ability to use leucrose as an acceptor, alternansucrase continues to transfer glucosyl units to leucrose, resulting in some unusual glucosyl-fructose oligosaccharides
-
-
?
sucrose + luteolin
luteolin-3'-O-alpha-D-glucopyranoside + luteolin-4'-O-alpha-D-glucopyranoside
sucrose + maltose
oligoalternan
sucrose + methyl alpha-D-allo-pyranoside
methyl alpha-D-glucopyranosyl-(1,6)-alpha-D-allopyranoside + alpha-D-glucopyranosyl-(1,6)-alpha-D-glucopyranosyl-(1,6)-alpha-D-allopyranoside + beta-D-fructofuranose
-
-
the product methyl alpha-D-glucopyranosyl-(1,6)-alpha-D-allopyranoside is subsequently glucosylated at position 6 to give rise to the trisaccharide methyl alpha-D-glucopyranosyl-(1,6)-alpha-D-glucopyranosyl-(1,6)-alpha-D-allopyranoside. Higher DP products are observed
-
?
sucrose + methyl alpha-D-galactopyranoside
methyl alpha-D-glucopyranosyl-(1,3)-alpha-D-galactopyranoside + methyl alpha-D-glucopyranosyl-(1,4)-alpha-D-galactopyranoside + beta-D-fructofuranose
-
-
two initial products methyl alpha-D-glucopyranosyl-(1,3)-alpha-D-galactopyranoside and methyl alpha-D-glucopyranosyl-(1,4)-alpha-D-galactopyranoside, in a 2.5:1 molar ratio
-
?
sucrose + methyl alpha-D-galactopyranoside
methyl alpha-D-glucopyranosyl-(1,4)-alpha-D-galactopyranoside + methyl alpha-D-glucopyranosyl-(1,3)-alpha-D-galactopyranoside + beta-D-fructofuranose
-
-
production of methyl alpha-D-glucopyranosyl-(1, 4)-alpha-D-galactopyranoside and methyl alpha-D-glucopyranosyl-(1,3)-alpha-D-galactopyranoside in the ratio 2.5:1
-
?
sucrose + methyl alpha-D-mannopyranoside
methyl alpha-D-glucopyranosyl-(1,6)-alpha-D-mannopyranoside + methyl-3,6-di-O-alpha-D-glucopyranosyl-alpha-D-mannopyranoside + beta-D-fructofuranose
-
-
the major initial acceptor product is methyl alpha-D-glucopyranosyl-(1,6)-alpha-D-mannopyranoside, but several minor products are also isolated and characterized, including a 3,6-di-O-substituted mannopyranoside
-
?
sucrose + methyl beta-D-galactopyranoside
?
-
poor substrate
-
-
?
sucrose + methyl beta-D-mannopyranoside
?
-
poor substrate
-
-
?
sucrose + methyl-alpha-D-glucoside
oligoalternan
sucrose + methyl-beta-D-glucopyranoside
methyl beta-isomaltoside + methyl beta-isomaltotrioside + methyl alpha-D-glucopyranosyl-(1,3)-alpha-D-glucopyranosyl-(1,6)-beta-D-glucopyranoside + beta-D-fructofuranose
-
-
the initial product arising is methyl beta-isomaltoside, which is subsequently glucosylated to yield methyl beta-isomaltotrioside and methyl alpha-D-glucopyranosyl-(1,3)-alpha-D-glucopyranosyl-(1,6)-beta-D-glucopyranoside
-
?
sucrose + octyl-alpha-D-glucopyranoside
?
-
-
-
-
?
additional information
?
-
2 sucrose
?
-
-
-
?
stevioside + sucrose
?
-
transglucosylation
the product is composed of mono-, di-, and triglucosylated stevioside and their isomers
-
?
stevioside + sucrose
?
-
transglucosylation
the product is composed of mono-, di-, and triglucosylated stevioside and their isomers
-
?
sucrose + alpha-D-glucan
?
-
-
-
?
sucrose + alpha-D-glucan
?
-
-
-
?
sucrose + alpha-D-glucan
alternating-1,6-1,3-alpha-D-glucan
-
-
-
?
sucrose + alpha-D-glucan
alternating-1,6-1,3-alpha-D-glucan
-
-
-
?
sucrose + alpha-D-glucan
alternating-1,6-1,3-alpha-D-glucan
-
-
-
-
?
sucrose + alpha-D-glucan
alternating-1,6-1,3-alpha-D-glucan
-
-
-
?
sucrose + alpha-D-glucan
alternating-1,6-1,3-alpha-D-glucan
-
-
-
?
sucrose + alpha-D-glucan
alternating-1,6-1,3-alpha-D-glucan
-
-
-
-
?
sucrose + alpha-D-glucan
alternating-1,6-1,3-alpha-D-glucan
-
-
-
-
?
sucrose + alpha-D-glucan
alternating-1,6-1,3-alpha-D-glucan
-
-
-
-
?
sucrose + alpha-D-glucan
alternating-1,6-1,3-alpha-D-glucan
-
-
the product is an insoluble D-glucan that consists of 76 mol% 1,3-alpha-linked glucose and 24 mol% 1,6-alpha-linked glucose
?
sucrose + alpha-D-glucan
alternating-1,6-1,3-alpha-D-glucan
-
-
glucan consists of 49.1 mol% 1,6-alpha-linked glucose and 33.9 mol% 1,3-alpha-linked glucose with 13.6 mol% terminal glucose and 3.3 mol% 1,3,6-alpha-branched glucose
?
sucrose + alpha-D-glucopyranosyl-(1,4)-L-glucose
?
-
-
-
-
?
sucrose + alpha-D-glucopyranosyl-(1,4)-L-glucose
?
-
-
-
-
?
sucrose + cellobiose
oligosaccharides
-
-
alpha-D-glucopyranosyl-(1,2)-(beta-D-glucopyranosyl-(1,4))-D-glucopyranose + alpha-D-glucopyranosyl-(1,6)-beta-D-glucopyranosyl-(1,4)-D-glucopyranose, the last compound in turn can be glycosylated leading to the synthesis of a tetrasaccharide with an additional alpha-(1,6)-linkage at the non-reducing end
?
sucrose + cellobiose
oligosaccharides
-
-
alpha-D-glucopyranosyl-(1,2)-(beta-D-glucopyranosyl-(1,4))-D-glucopyranose + alpha-D-glucopyranosyl-(1,6)-beta-D-glucopyranosyl-(1,4)-D-glucopyranose, the last compound in turn can be glycosylated leading to the synthesis of a tetrasaccharide with an additional alpha-(1,6)-linkage at the non-reducing end
?
sucrose + D-tagatose
alpha-D-glucopyranosyl-(1,1)-beta-D-tagatopyranose
-
-
-
-
?
sucrose + D-tagatose
alpha-D-glucopyranosyl-(1,1)-beta-D-tagatopyranose
-
-
-
-
?
sucrose + gentiobiose
?
-
gentiobiose is better as an acceptor than isomaltose, acceptor products from gentiobiose, form in good yields (nearly 90% in unoptimized reactions). The initial product is a single trisaccharide, alpha-D-Glcp-(1-6)-beta-D-Glcp-(1-6)-D-Glc. Two tetrasaccharides are formed in approximately equal quantities: alpha-D-Glcp-(1-3)-alpha-D-Glcp-(1-6)-beta-D-Glcp-(1-6)-D-Glc and alpha-D-Glcp-(1-6)-alpha-D-Glcp-(1-6)-beta-D-Glcp-(1-6)-D-Glc. One pentasaccharide is isolated from the reaction mixture, alpha-D-Glcp-(1-6)-alpha-D-Glcp-(1-3)-alpha-D-Glcp-(1-6)-beta-D-Glcp-(1-6)-D-Glc
-
-
?
sucrose + gentiobiose
?
-
gentiobiose is better as an acceptor than isomaltose, acceptor products from gentiobiose, form in good yields (nearly 90% in unoptimized reactions). The initial product is a single trisaccharide, alpha-D-Glcp-(1-6)-beta-D-Glcp-(1-6)-D-Glc. Two tetrasaccharides are formed in approximately equal quantities: alpha-D-Glcp-(1-3)-alpha-D-Glcp-(1-6)-beta-D-Glcp-(1-6)-D-Glc and alpha-D-Glcp-(1-6)-alpha-D-Glcp-(1-6)-beta-D-Glcp-(1-6)-D-Glc. One pentasaccharide is isolated from the reaction mixture, alpha-D-Glcp-(1-6)-alpha-D-Glcp-(1-3)-alpha-D-Glcp-(1-6)-beta-D-Glcp-(1-6)-D-Glc
-
-
?
sucrose + isomaltose
?
-
-
-
-
?
sucrose + isomaltose
?
-
-
-
-
?
sucrose + isomaltose
oligoalternan
-
-
-
?
sucrose + isomaltose
oligoalternan
-
-
-
?
sucrose + L-glucose
alpha-D-glucopyranosyl-(1,6)-alpha-D-glucopyranosyl-(1,4)-L-glucose
-
-
-
-
?
sucrose + L-glucose
alpha-D-glucopyranosyl-(1,6)-alpha-D-glucopyranosyl-(1,4)-L-glucose
-
-
-
-
?
sucrose + luteolin
luteolin-3'-O-alpha-D-glucopyranoside + luteolin-4'-O-alpha-D-glucopyranoside
-
8% conversion
-
-
?
sucrose + luteolin
luteolin-3'-O-alpha-D-glucopyranoside + luteolin-4'-O-alpha-D-glucopyranoside
-
8% conversion
-
-
?
sucrose + luteolin
luteolin-3'-O-alpha-D-glucopyranoside + luteolin-4'-O-alpha-D-glucopyranoside
-
8% conversion
-
-
?
sucrose + maltose
?
maltose undergoes alpha-1,6 glucosylation alone to give the oligodextran of DP3 (OD3 panose, alpha-D-Glcp-(1->6)-alpha-D-Glcp-(1->4)-D-Glc), which can be further elongated at either the O6 or the O3 position of the nonreducing unit to give the structures OD4 (alpha-D-Glcp-(1->6)-alpha-D-Glcp-(1->6)-alpha-D-Glcp-(1->4)-D-Glc) and OA4 (alpha-D-Glcp-(1->3)-alpha-D-Glcp-(1->6)-alpha-D-Glcp-(1->4)-D-Glc), respectively. The oligosaccharide OA4 is quick to appear at the beginning of the reaction (1 min) and accumulates at a much higher level than OD4, indicating that panose is preferentially elongated with an alpha-1,3 linkage. A very small peak of OD5 originating from OD4 only appears toward the end of the reaction (after about 30 min) and is not elongated further (no OD6 is found). As soon as the OA4 starts to accumulate, it is efficiently converts to OA5 (alpha-D-Glcp-(1->6)-alpha-D-Glcp-(1->3)-alpha-D-Glcp-(1->6)-alpha-D-Glcp-(1->4)-D-Glc). The OA5 itself can act as an acceptor for the formation of two OA6s (alpha-D-Glcp-(1->6)-alpha-D-Glcp-(1->6)-alpha-D-Glcp-(1->3)-alpha-D-Glcp-(1->6)-alpha-D-Glcp-(1->4)-DGlc and alpha-D-Glcp-(1->3)-alpha-D-Glcp-(1->6)-alpha-D-Glcp-(1->3)-alpha-D-Glcp-(1->6)-alpha-D-Glcp-(1->4)-D-Glc)
-
-
?
sucrose + maltose
?
maltose undergoes alpha-1,6 glucosylation alone to give the oligodextran of DP3 (OD3 panose, alpha-D-Glcp-(1->6)-alpha-D-Glcp-(1->4)-D-Glc), which can be further elongated at either the O6 or the O3 position of the nonreducing unit to give the structures OD4 (alpha-D-Glcp-(1->6)-alpha-D-Glcp-(1->6)-alpha-D-Glcp-(1->4)-D-Glc) and OA4 (alpha-D-Glcp-(1->3)-alpha-D-Glcp-(1->6)-alpha-D-Glcp-(1->4)-D-Glc), respectively. The oligosaccharide OA4 is quick to appear at the beginning of the reaction (1 min) and accumulates at a much higher level than OD4, indicating that panose is preferentially elongated with an alpha-1,3 linkage. A very small peak of OD5 originating from OD4 only appears toward the end of the reaction (after about 30 min) and is not elongated further (no OD6 is found). As soon as the OA4 starts to accumulate, it is efficiently converts to OA5 (alpha-D-Glcp-(1->6)-alpha-D-Glcp-(1->3)-alpha-D-Glcp-(1->6)-alpha-D-Glcp-(1->4)-D-Glc). The OA5 itself can act as an acceptor for the formation of two OA6s (alpha-D-Glcp-(1->6)-alpha-D-Glcp-(1->6)-alpha-D-Glcp-(1->3)-alpha-D-Glcp-(1->6)-alpha-D-Glcp-(1->4)-DGlc and alpha-D-Glcp-(1->3)-alpha-D-Glcp-(1->6)-alpha-D-Glcp-(1->3)-alpha-D-Glcp-(1->6)-alpha-D-Glcp-(1->4)-D-Glc)
-
-
?
sucrose + maltose
?
-
-
-
-
?
sucrose + maltose
?
-
alternansucrase acceptor products from maltose do not contain dextran-type linkage sequences. Instead, the product series is comprised of an alternan-type linkage sequence, with some pairs of consecutive alpha-(1,6) linkages in the evennumbered members of the series. The distribution and sequence of linkages is apparently kinetically controlled. Branch formation is not detected below DP 8. Alternansucrase forms alpha-(1,3) linkages only when the acceptor is a-(1,6)-linked, thereby prohibiting the formation of sequences of alpha-(1,3) linkages. Furthermore, the enzyme appears not to make products containing more than two sequential alpha-(1,6) linkages, thereby prohibiting the formation of dextran-like linkage sequences
-
-
?
sucrose + maltose
?
-
-
-
-
?
sucrose + maltose
?
-
alternansucrase acceptor products from maltose do not contain dextran-type linkage sequences. Instead, the product series is comprised of an alternan-type linkage sequence, with some pairs of consecutive alpha-(1,6) linkages in the evennumbered members of the series. The distribution and sequence of linkages is apparently kinetically controlled. Branch formation is not detected below DP 8. Alternansucrase forms alpha-(1,3) linkages only when the acceptor is a-(1,6)-linked, thereby prohibiting the formation of sequences of alpha-(1,3) linkages. Furthermore, the enzyme appears not to make products containing more than two sequential alpha-(1,6) linkages, thereby prohibiting the formation of dextran-like linkage sequences
-
-
?
sucrose + maltose
oligoalternan
-
-
-
-
?
sucrose + maltose
oligoalternan
-
-
panose is the first acceptor product
?
sucrose + maltose
oligoalternan
-
-
panose is the first acceptor product
?
sucrose + maltose
oligoalternan
-
-
-
-
?
sucrose + methyl-alpha-D-glucoside
oligoalternan
-
-
-
?
sucrose + methyl-alpha-D-glucoside
oligoalternan
-
-
-
?
sucrose + myricetin
?
-
49% conversion
-
-
?
sucrose + myricetin
?
-
49% conversion
-
-
?
sucrose + myricetin
?
-
49% conversion
-
-
?
sucrose + quercetin
?
-
4% conversion
-
-
?
sucrose + quercetin
?
-
4% conversion
-
-
?
sucrose + quercetin
?
-
4% conversion
-
-
?
sucrose + raffinose
?
-
the alternansucrase-catalyzed acceptor reaction with raffinose gives a gravimetric yield of alternan of 9.5% relative to the weight of sucrose, indicating that 20% of the glucosyl units are incorporated into alternan, and 80% into oligosaccharide acceptor products. The main products are the tetrasaccharides alpha-D-Glcp-(1-3)-alpha-D-Galp-(1-6)-alpha-D-Glcp-(1-2)-beta-D-Fruf and alpha-D-Glcp-(1-4)-alpha-D-Galp-(1-6)-alpha-D-Glcp-(1-2)-beta-D-Fruf in ratios ranging from 4:1 to 9:1, along with lesser amounts of alpha-D-Glcp-(1-6)-alpha-D-Galp-(1-6)-alpha-D-Glcp-(1-2)-beta-D-Fruf. Pentasaccharides, hexasaccharides and higher oligosaccharides are also produced
-
-
?
sucrose + raffinose
?
-
the alternansucrase-catalyzed acceptor reaction with raffinose gives a gravimetric yield of alternan of 9.5% relative to the weight of sucrose, indicating that 20% of the glucosyl units are incorporated into alternan, and 80% into oligosaccharide acceptor products. The main products are the tetrasaccharides alpha-D-Glcp-(1-3)-alpha-D-Galp-(1-6)-alpha-D-Glcp-(1-2)-beta-D-Fruf and alpha-D-Glcp-(1-4)-alpha-D-Galp-(1-6)-alpha-D-Glcp-(1-2)-beta-D-Fruf in ratios ranging from 4:1 to 9:1, along with lesser amounts of alpha-D-Glcp-(1-6)-alpha-D-Galp-(1-6)-alpha-D-Glcp-(1-2)-beta-D-Fruf. Pentasaccharides, hexasaccharides and higher oligosaccharides are also produced
-
-
?
additional information
?
-
-
LC/MS product analysis
-
-
?
additional information
?
-
alternansucrase enzyme from Leuconostoc citreum SK24.002 is used to produce di-glycosyl-stevioside through acceptor reaction. Identification of the di-glycosyl-stevioside structure as 13-([alpha-D-glucopyranosyl-(1->3)-alpha-D-glucopyranosyl-(1->6)-beta-D glucopyranosyl-(1->2)-beta-D-glucopyranosyl]oxy)kaur-16-en-19-oic acid beta-D glucopyranosyl ester, method optimization and evaluation, overview
-
-
-
additional information
?
-
for the docking calculations, a model of the ASRDELTA2 glucosyl-enzyme intermediate is constructed based on the high resolution structure of the GH13 covalent intermediate (PDB ID 1S46). Maltose is the best known acceptor for ASR
-
-
-
additional information
?
-
product analysis, determination of alternan nanoparticle size distribution by dynamic light scattering
-
-
-
additional information
?
-
-
product analysis, determination of alternan nanoparticle size distribution by dynamic light scattering
-
-
-
additional information
?
-
product analysis, determination of alternan nanoparticle size distribution by dynamic light scattering
-
-
-
additional information
?
-
for the docking calculations, a model of the ASRDELTA2 glucosyl-enzyme intermediate is constructed based on the high resolution structure of the GH13 covalent intermediate (PDB ID 1S46). Maltose is the best known acceptor for ASR
-
-
-
additional information
?
-
-
LC/MS product analysis
-
-
?
additional information
?
-
alternansucrase enzyme from Leuconostoc citreum SK24.002 is used to produce di-glycosyl-stevioside through acceptor reaction. Identification of the di-glycosyl-stevioside structure as 13-([alpha-D-glucopyranosyl-(1->3)-alpha-D-glucopyranosyl-(1->6)-beta-D glucopyranosyl-(1->2)-beta-D-glucopyranosyl]oxy)kaur-16-en-19-oic acid beta-D glucopyranosyl ester, method optimization and evaluation, overview
-
-
-
additional information
?
-
-
methyl D-allopyrano-sides are glucosylated primarily at position 6, yielding methyl alpha-D-glucopyranosyl-(1,6)-D-allopyranosides. The latter subsequently gave rise to methyl alpha-D-glucopyranosyl-(1,6)-alpha-D-glucopyranosyl-(1,6)-D-allopyranosides. The methyl alpha-D-hexopyranosides are better acceptors than the corresponding beta-glycosides
-
-
?
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evolution
the enzyme belongs to the CAZy glycoside hydrolase family 70 (GH70)
evolution
the enzyme belongs to the glycoside hydrolase family 70 (GH70)
evolution
-
the enzyme belongs to the glycoside hydrolase family 70 (GH70)
-
evolution
-
the enzyme belongs to the CAZy glycoside hydrolase family 70 (GH70)
-
physiological function
alternansucrase (ASR) is an alpha-transglucosylase capable of catalyzing high and low molar mass alternan, an alpha-glucan comprising alternating alpha-1,3 and alpha-1,6 linkages in its linear chain. ASR also catalyzes transglucosylation from sucrose to many different types of sugar acceptors: methyl-alpha-D-glucoside, maltose, maltodextrin, maltitol, isomaltooligosaccharides, cellobiose, melibiose, raffinose, gentiobiose, and lactose, and produces glucosylated products showing interesting prebiotic properties
physiological function
alternansucrase catalyses the sequential transfer of glucose residues from sucrose onto another sucrose molecule to form a long chain polymer, known as alternan. The insoluble polymer alternan has alternating alpha-1,6 and alpha-1,3 glycosidic linkages as has been judged by using 13C NMR spectroscopy and methylation analysis, partially hydrolysed product analysis, and enzymatic analysis
physiological function
-
alternansucrase (ASR) is an alpha-transglucosylase capable of catalyzing high and low molar mass alternan, an alpha-glucan comprising alternating alpha-1,3 and alpha-1,6 linkages in its linear chain. ASR also catalyzes transglucosylation from sucrose to many different types of sugar acceptors: methyl-alpha-D-glucoside, maltose, maltodextrin, maltitol, isomaltooligosaccharides, cellobiose, melibiose, raffinose, gentiobiose, and lactose, and produces glucosylated products showing interesting prebiotic properties
-
physiological function
-
alternansucrase catalyses the sequential transfer of glucose residues from sucrose onto another sucrose molecule to form a long chain polymer, known as alternan. The insoluble polymer alternan has alternating alpha-1,6 and alpha-1,3 glycosidic linkages as has been judged by using 13C NMR spectroscopy and methylation analysis, partially hydrolysed product analysis, and enzymatic analysis
-
additional information
analysis of structural determinants involved in the linkage specificity of alternansucrase (ASR), overview. ASR displays two different acceptor subsites in the prolongation of its subsites -1 and +1. The first one is defined by Trp675, a residue of subsite +2, and orients acceptor binding exclusively toward alpha-1,6 linkage synthesis. The second binding site comprises Asp772 and Trp543, two residues defining the +2' and +3' subsites, respectively, which are critical for alpha-1,3 linkage formation. It is proposed that the interplay between these two acceptor sites controls alternance. The enzyme is predicted to adopt the same fold as the other glucansucrases and the same alpha-retaining mechanism involving the contribution of Asp635, Glu673, and Asp767. These amino acids play the role of, respectively, the nucleophile, acid/base catalyst, and transition state stabilizer (TSS) implicated in the formation of the beta-D-glucosyl-enzyme intermediate. Kinetic study conducted with ASR C-APY-del shows that both high molar mass and low molar mass alternan populations are formed in the early stage of the reaction, suggesting that ASR follows a semiprocessive mechanism of polymerization. Three-dimensional structure analysis, overview
additional information
catalytic mechanism of di-glycosyl-stevioside synthesis, overview
additional information
-
catalytic mechanism of di-glycosyl-stevioside synthesis, overview
-
additional information
-
analysis of structural determinants involved in the linkage specificity of alternansucrase (ASR), overview. ASR displays two different acceptor subsites in the prolongation of its subsites -1 and +1. The first one is defined by Trp675, a residue of subsite +2, and orients acceptor binding exclusively toward alpha-1,6 linkage synthesis. The second binding site comprises Asp772 and Trp543, two residues defining the +2' and +3' subsites, respectively, which are critical for alpha-1,3 linkage formation. It is proposed that the interplay between these two acceptor sites controls alternance. The enzyme is predicted to adopt the same fold as the other glucansucrases and the same alpha-retaining mechanism involving the contribution of Asp635, Glu673, and Asp767. These amino acids play the role of, respectively, the nucleophile, acid/base catalyst, and transition state stabilizer (TSS) implicated in the formation of the beta-D-glucosyl-enzyme intermediate. Kinetic study conducted with ASR C-APY-del shows that both high molar mass and low molar mass alternan populations are formed in the early stage of the reaction, suggesting that ASR follows a semiprocessive mechanism of polymerization. Three-dimensional structure analysis, overview
-
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Cote, G.L.; Robyt, J.F.
Isolation and partial characterization of an extracellular glucansucrase from Leuconostoc mesenteroides NRRL B-1355 that synthesizes an alternating (1-6),(1-3)-alpha-D-glucan
Carbohydr. Res.
101
57-74
1982
Leuconostoc mesenteroides, Leuconostoc mesenteroides NRRL B-1355
brenda
Lopez-Munguia, A.; Pelenc, V.; Remaud, M.; Biton, J.; Michel, J.M.; Lang, C.; Paul, F.; Monsan, P.
Production and purification of alternansucrase, a glucosyltransferase from Leuconostoc mesenteroides NRRL B-1355, for the synthesis of oligoalternan
Enzyme Microb. Technol.
15
77-85
1993
Leuconostoc mesenteroides, Leuconostoc mesenteroides NRRL B-1355
-
brenda
Mukasa, H.; Shimamura, A.; Tsumori, H.
Purification and characterization of cell-associated glucosyltransferase synthesizing insoluble glucan from Streptococcus mutans serotype c
J. Gen. Microbiol.
135
2055-2063
1989
Streptococcus mutans
brenda
Tsumori, H.; Shimamura, A.; Mukasa, H.
Purification and properties of extracellular glucosyltransferase synthesizing 1,6-, 1,3-alpha-D-glucan from Streptococcus mutans serotype a
J. Gen. Microbiol.
131
3347-3353
1985
Streptococcus mutans
brenda
Arguello-Morales, M.A.; Remaud-Simeon, M.; Pizzut, S.; Sarcabal, P.; Willemot, R.M.; Monsan, P.
Sequence analysis of the gene encoding alternansucrase, a sucrose glucosyltransferase from Leuconostoc mesenteroides NRRL B-1355
FEMS Microbiol. Lett.
182
81-85
2000
Leuconostoc mesenteroides, Leuconostoc mesenteroides NRRL B-1355
brenda
Zahnley, J.C.; Smith, M.R.
Cellular association of glucosyltransferases in Leuconostoc mesenteroides and effects of detergent on cell association
Appl. Biochem. Biotechnol.
87
57-70
2000
Leuconostoc mesenteroides
brenda
Smith, M.R.; Zahnley, J.C.
Leuconostoc mesenteroides B-1355 mutants producing alternansucrases exhibiting decreases in apparent molecular mass
Appl. Environ. Microbiol.
63
581-586
1997
Leuconostoc mesenteroides, Leuconostoc mesenteroides NRRL B-1355
brenda
Kim, D.; Kim, Y.M.; Park, M.R.; Park, D.H.
Modification of Acetobacter xylinum bacterial cellulose using dextransucrase and alternansucrase
J. Microbiol. Biotechnol.
9
704-708
1999
Leuconostoc mesenteroides
-
brenda
Richard, G.; Morel, S.; Willemot, R.M.; Monsan, P.; Remaud-Simeon, M.
Glucosylation of alpha-butyl- and alpha-octyl-D-glucopyranosides by dextransucrase and alternansucrase from Leuconostoc mesenteroides
Carbohydr. Res.
338
855-864
2003
Leuconostoc mesenteroides
brenda
Arguello Morales, M.A.; Remaud-Simeon, M.; Willemot, R.M.; Vignon, M.R.; Monsan, P.
Novel oligosaccharides synthesized from sucrose donor and cellobiose acceptor by alternansucrase
Carbohydr. Res.
331
403-411
2001
Leuconostoc mesenteroides, Leuconostoc mesenteroides NRRL B-23192
brenda
Cote, G.L.; Dunlap, C.A.
Alternansucrase acceptor reactions with methyl hexopyranosides
Carbohydr. Res.
338
1961-1967
2003
Leuconostoc mesenteroides
brenda
Cote, G.L.; Dunlap, C.A.; Appell, M.; Momany, F.A.
Alternansucrase acceptor reactions with D-tagatose and L-glucose
Carbohydr. Res.
340
257-262
2005
Leuconostoc mesenteroides, Leuconostoc mesenteroides NRRL B-21297
brenda
Kok-Jacon, G.A.; Vincken, J.P.; Suurs, L.C.; Wang, D.; Liu, S.; Visser, R.G.
Expression of alternansucrase in potato plants
Biotechnol. Lett.
29
1135-1142
2007
Leuconostoc mesenteroides, Leuconostoc mesenteroides NRRL B-1355
brenda
Cote, G.L.; Sheng, S.
Penta-, hexa-, and heptasaccharide acceptor products of alternansucrase
Carbohydr. Res.
341
2066-2072
2006
Leuconostoc mesenteroides, Leuconostoc mesenteroides NRRL B-21297
brenda
Bertrand, A.; Morel, S.; Lefoulon, F.; Rolland, Y.; Monsan, P.; Remaud-Simeon, M.
Leuconostoc mesenteroides glucansucrase synthesis of flavonoid glucosides by acceptor reactions in aqueous-organic solvents
Carbohydr. Res.
341
855-863
2006
Leuconostoc mesenteroides, Leuconostoc mesenteroides NRRL B-23192
brenda
Joucla, G.; Pizzut, S.; Monsan, P.; Remaud-Simeon, M.
Construction of a fully active truncated alternansucrase partially deleted of its carboxy-terminal domain
FEBS Lett.
580
763-768
2006
Leuconostoc mesenteroides (Q9RE05)
brenda
Iliev, I.; Vassileva, T.; Ignatova, C.; Ivanova, I.; Haertle, T.; Monsan, P.; Chobert, J.M.
Gluco-oligosaccharides synthesized by glucosyltransferases from constitutive mutants of Leuconostoc mesenteroides strain Lm 28
J. Appl. Microbiol.
104
243-250
2008
Leuconostoc mesenteroides
brenda
Colte, G.; Sheng, S.; Dunlap, C.
Alternansucrase acceptor products
Biocatal. Biotransform.
26
161-168
2008
Leuconostoc mesenteroides
-
brenda
Cote, G.L.
Acceptor products of alternansucrase with gentiobiose. Production of novel oligosaccharides for food and feed and elimination of bitterness
Carbohydr. Res.
344
187-190
2009
Leuconostoc mesenteroides, Leuconostoc mesenteroides NRRL B-21297
brenda
Cote, G.L.; Dunlap, C.A.; Vermillion, K.E.
Glucosylation of raffinose via alternansucrase acceptor reactions
Carbohydr. Res.
344
1951-1959
2009
Leuconostoc mesenteroides, Leuconostoc mesenteroides NRRL B-21297
brenda
Bounaix, M.S.; Gabriel, V.; Robert, H.; Morel, S.; Remaud-Simeon, M.; Gabriel, B.; Fontagne-Faucher, C.
Characterization of glucan-producing Leuconostoc strains isolated from sourdough
Int. J. Food Microbiol.
144
1-9
2010
Leuconostoc mesenteroides, Leuconostoc citreum (D3JBW0), Leuconostoc citreum (D3JC16), Leuconostoc citreum (D3JC18), Leuconostoc citreum, Leuconostoc mesenteroides G15, Leuconostoc citreum C-11 (D3JC18), Leuconostoc citreum E16 (D3JBW0), Leuconostoc citreum B2 (D3JC16)
brenda
Hernandez-Hernandez, O.; Cote, G.L.; Kolida, S.; Rastall, R.A.; Sanz, M.L.
In vitro fermentation of alternansucrase raffinose-derived oligosaccharides by human gut bacteria
J. Agric. Food Chem.
59
10901-10906
2011
Leuconostoc mesenteroides, Leuconostoc mesenteroides NRRL B-21297
brenda
Musa, A.; Miao, M.; Zhang, T.; Jiang, B.
Biotransformation of stevioside by Leuconostoc citreum SK24.002 alternansucrase acceptor reaction
Food Chem.
146
23-29
2014
Leuconostoc citreum, Leuconostoc citreum SK24.002
brenda
Molina, M.; Moulis, C.; Monties, N.; Pizzut-Serin, S.; Guieysse, D.; Morel, S.; Cioci, G.; Remaud-Simeon, M.
Deciphering an undecided enzyme investigations of the structural determinants involved in the linkage specificity of alternansucrase
ACS Catal.
9
2222-2237
2019
Leuconostoc citreum (D3JC16), Leuconostoc citreum NRRL B-1355 (D3JC16)
-
brenda
Musa, A.; Jiang, B.; Ma, H.; Gasmalla, M.A.A.; Abdalhai, M.H.; Al-alfarga, A.
Di-glycosyl-stevioside production via Leuconostoc citreum sk24.002 alternansucrase enzymatic reaction and structural characterization
J. Food Meas. Charact.
13
1159-1165
2019
Leuconostoc citreum (D3JC16), Leuconostoc citreum SK24.002 (D3JC16)
-
brenda
Wangpaiboon, K.; Padungros, P.; Nakapong, S.; Charoenwongpaiboon, T.; Rejzek, M.; Field, R.A.; Pichyangkura, R.
An alpha-1,6-and alpha-1,3-linked glucan produced by Leuconostoc citreum ABK-1 alternansucrase with nanoparticle and film-forming properties
Sci. Rep.
8
8340
2018
Leuconostoc citreum (D3JC16), Leuconostoc citreum, Leuconostoc citreum ABK-1 (D3JC16)
brenda