TAP-tagged septin proteins are cleaved from the magnetic beads during the KAT assay. Multiple sites of acetylation on four of the five yeast mitotic septins, Cdc3, Cdc10, Cdc12 and Shs1 are discovered. In addition to acetylating itself, NuA4 is capable of acetylating Cdc3, Cdc12 and Shs1 in vitro, acetylation on Cdc10 is not detected
RTK1, naturally occurring hydroxynaphthoquinone, isolated from Plumbago rosea roots, inhibits histone acetylation, and induces apoptosis at higher concentrations, it inhibits p300/CBP-mediated acetylation of p53 lysine 373 non-competitively, 25 microM inhibit histidine-tagged recombinant p300 with purified human HeLa core histone as substrate by about 60% compared to control; RTK1, naturally occurring hydroxynaphthoquinone, isolated from Plumbago rosea roots, it does not inhibit PCAF acetylation of p53 lysine 320 in vivo in HEK-293 cells (pretreated with acetylation inducer doxorubicin), but 10, 25, and 50 microM inhibit FLAG-tagged recombinant PCAF in vitro (30°C) with purified human HeLa core histone as substrate
no inhibition of p300 by 5-methoxy-2-methyl-1,4-naphthoquinone (RTK2, alkyl substitution of hydroxyl group), 5-ethoxy-2-methyl-1,4-naphthoquinone (RTK3, alkyl substitution of hydroxyl group), 5-isopropoxy-2-methyl-1,4-naphthoquinone (RTK4, alkyl substitution of hydroxyl group), and 5-[2-(dimethylamino)-ethoxy]-2-methyl-1,4-naphthoquinone (RTK10, N,N-dimethylamine substitution of hydroxyl group), less than 10% inhibition with 6-methyl-5,8-dioxo-5,8-dihydronaphthalen-1-yl acetate (RTK5, acetyl substitution of hydroxyl group), 6-methyl-5,8-dioxo-5,8-dihydronaphthalen-1-yl methanesulfonate (RTK6, sulfonyl substitution of hydroxyl group), 2-methyl-5-(2-piperidin-1-ylethoxy)-1,4-naphthoquinone (RTK7, piperidine substitution of hydroxyl group), 2-methyl-5-(2-morpholin-4-ylethoxy)-1,4-naphthoquinone (RTK8, morpholine substitution of hydroxyl group), and ethyl [(6-methyl-5,8-dioxo-5,8-dihydronaphthalen-1-yl)-oxy]acetate (RTK9, ester substitution of hydroxyl group)
Rtt109 associates with chaperone Vps75 (H3K9 and H3K23 acetylation in vivo) or Asf1 (H3K56 acetylation in vivo), stimulation of catalytic activity by chaperones, R55109 stimulates histone deposition by the chaperones, the complex of enzyme + chaperone alters substrate specificity, targets to specific loci, enhances acetyltransferase activity, restricts access of non-target proteins, and coordinates the multiple enzyme activities of the complex
the phosphorylation of the receptor-interacting protein 140 (RIP140) by extracellular-signal-related kinase 2 (Erk2) stimulates p300 acetyltransferase to acetylate the RIP140's lysine 158 and lysine 287 residues
Hep-G2 hepatocarcinoma cells: with 5 microM plumbagin 50% reduction of histone H3 acetylation, with 25 microM 90% reduction, significant overall acetylation status of histones, prominent reduction in H3 and H4, immunofluorescence analysis (anti-acetylated histone H3 polyclonal antibodies) of HeLa cells (treated with deacetylase inhibitors to induce histone acetylation) confirm the inhibitory effect of plumbagin with 5 microM inhibitor, with 25 microM almost complete reduction in acetylation level
significant decrease of histone acetylation in plumbagin treated mouse liver in vivo 6 h after intraperitoneal injection of 25 mg plumbagin/kg body mass
novel taste learning elicited biphasic (acute and long-tasting) activation of two distinct lysine acetyltransferase activities along with the EPK/MAPK cascade in insular cortex
beta-site amyloid precursor protein-cleaving enzyme 1 (BACE1) is acetylated in seven lysine residues that face the lumen of the ER and ER Golgi intermediate compartment (ERGIC)
a TgGCN5-A null mutant is deficient in responding to alkaline pH, a common stress used to induce bradyzoite differentiation in vitro. A genome-wide analysis of the Toxoplasma transcriptional response to alkaline pH stress shows that parasites deleted for TgGCN5-A fail to up-regulate 74% of the stress response genes that are induced 2fold or more in wild-type. TgGCN5-A knockout is also incapable of up-regulating key marker genes expressed during development of the latent cyst form, and is impaired in its ability to recover from alkaline stress
acetyltransferase-deficient NuA4 mutants have defects in septin collar formation resulting in the development of elongated buds through the Swe1-dependent morphogenesis checkpoint
DELTAabl mutants of Methanococcus maripaludis no longer produced Nepsilon-acetyl-beta-lysine and are incapable of growth at high salt concentrations, indicating that the abl operon is essential for Nepsilon-acetyl-beta-lysine synthesis
Esa1 mediates increased H4 acetylation and enhanced chromatin remodeling complex RSC occupancy and histone eviction in coding sequences and stimulates the rate of transcription elongation by polymerase II
specific lysine 158 and lysine 287 acetylation of RIP140 (receptor-interacting protein 140) which is a co-regulator for many transcription factors, acetylation directly enhances the regulator's trans-repressive activity, role in fat accumulation
the acetyltransferases cyclic adenosine monophosphate response element-binding binding protein (CBP) and acetyltransferase p300 attenuate transcriptional activity of the mineralocorticoid receptor through its acetylation
site directed mutagenesis of lysine 1358 of the p300 acetyltransferase domain reveals that inhibitor binds via a hydrogen bond to this lysine residue in the wild-type, in the mutant no binding leads to total loss of acetyltransferase activity
esa1 mutant (reduced histone H4 acetylation) at 36°C (restrictive temperature) is sensitive to 6-azauracil (inhibitor impeding elongation by lowering nucleotide pools) but shows little effect at 30°C (permissive temperature), gene length dependent defects in transcription
Lysine 158 and 287 in the RIP140 (receptor-interacting protein 140) are targets for acetylation by p300 acetyltransferase as shown by site directed mutagenesis of these residues, lysine 158 and 287 replaced by glutamine (mimicking acetylation) turn the RIP140 repressive in the absence of p300, while the alanine replacements (preventing acetylation) prevents responsiveness to p300, the double mutants show stronger effects in the same directions, while lysine 111 and 311 seem not to be targets for the p300 acetyltransferase. Mitogen-activated protein kinase (MAPK) target mutants that mimic (T202E/T207E) or abolish (T202A/T207A) threonine phosphorylation of RIP140 enhance or reduce the stimulation of p300 acetyltransferase, respectively, extracellular-signal-related kinase 2 (Erk2) is identified as the responsible MAPK in this pathway
in human embryonic kidney 293 cells, overexpression of cyclic adenosine monophosphate response element-binding binding protein, CBP, or p300, but not p300/CBP-associated factor, increases mineralocorticoid receptor acetylation and decreases expression of mineralocorticoid receptor target genes
double mutant esa1/gcn5 at 36°C is very sensitive to 6-azauracil (inhibitor impeding elongation by lowering nucleotide pools) and shows littler but still strong effect at 30°C (permissive temperature), gene length dependent defects in transcription
gcn5 deletion mutant (reduced H3 acetylation) at 36°C (restrictive temperature) is very sensitive to 6-azauracil (inhibitor impeding elongation by lowering nucleotide pools) but shows less effect at 30°C (permissive temperature), gene length dependent defects in transcription
cells are lysed after centrifugation in 100 mM Tris-HCl buffer, pH 7.6, immunoprecipitation, affinity purification with ProFound c-Myc-Tag IP/Co-IP kit, subcellular fractionation of homogenized cells in 10 mM triethanolamine, 10 mM acetic acid, 250 mM sucrose, 1 mM EDTA, and 1 mM dithiothreitol, pH 7.4, centrifuged, membrane pellet resuspended in 5% Nycodenz and layered on top of a Nycodenz solution gradient in 10 mM HEPES, pH 7.4, fractions collected and further concentrated by ultracentrifugation
cells are washed with PBS, harvested in Tris-HCl, pH 7.4, containing 0.5% deoxycholic acid, 150 mM NaCl, 0.1% SDS, 4 mM EDTA, and 1% NP-40, with a protease inhibitor cocktail, 1 mM PMSF, 1 mM sodium fluoride, and 1 mM sodium orthovanadate, centrifugation, supernatant subjected to SDS-PAGE, or for immunoprecipitation lysed cells are collected with 50 mM Tris-HCl, pH 8.0, with 10% glycerol, 100 mM NaCl, 1 mM EDTA, and 0.1% NP-40 with proteinase inhibitor cocktail, 1 mM PMSF, 1 mM sodium fluoride, and 1 mM sodium orthovanadate, incubation with antibodies and G beads, washing, elution of proteins by boiling in 2X Laemmli loading buffer
transfection of COS-1 cells (African green monkey cell line) with plasmids of wild-type enzyme, deficient enzyme or single site mutants fused to reporter genes
expression of the abl operon is strictly salt dependent. Expression of ablA and ablB at the standard NaCl concentration of 38.5 mM is not detectable, but it increases drastically with increasing salt concentrations in the growth medium. The level of transcription of the abl operon is identical in cells grown with 400 or 800 mM NaCl
expression of the abl operon is strictly salt dependent. Expression of ablA and ablB at the standard NaCl concentration of 38.5 mM is not detectable, but it increases drastically with increasing salt concentrations in the growth medium. The level of transcription of the abl operon is identical in cells grown with 400 or 800 mM NaCl
expression of the abl operon is strictly salt dependent. Expression of ablA and ablB at the standard NaCl concentration of 38.5 mM is not detectable, but it increases drastically with increasing salt concentrations in the growth medium. The level of transcription of the abl operon is identical in cells grown with 400 or 800 mM NaCl
pathogenesis of late-onset Alzheimer disease, post-translational regulation of beta-site amyloid precursor protein-cleaving enzyme 1 (BACE1) levels, a membrane protein that acts as the rate-limiting enzyme in the generation of Alzheimer disease amyloid beta-peptide, acetylation protect the protein from degradation
cyclic adenosine monophosphate response element-binding binding protein and p300 are lysine acetyltransferases responsible for the regulation of mineralocorticoid receptor providing therapeutic targets for the treatment of hypertension
Increased histone acetyltransferase and lysine acetyltransferase activity and biphasic activation of the ERK/RSK cascade in insular cortex during novel taste learning
Biochemical and thermodynamic analyses of Salmonella enterica Pat, a multidomain, multimeric N-lysine acetyltransferase involved in carbon and energy metabolism
Seo, M.; Song, M.; Seok, Y.M.; Kang, S.H.; Lee, H.A.; Sohn, U.D.; Kim, I.K.
Lysine acetyltransferases cyclic adenosine monophosphate response element-binding binding protein and acetyltransferase p300 attenuate transcriptional activity of the mineralocorticoid receptor through its acetylation