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4 S-adenosyl-L-methionine + quercetin
4 S-adenosyl-L-homocysteine + 3,3',5,7-tetramethoxyquercetin
whole-cell biocatalysis using CdFOMT5 expressed in Escherichia coli cells is performed using quercetin as a substrate, and 3,3',5,7-tetramethylated quercetin is obtained as the final product
-
-
?
S-adenosyl-L-methionine + 3',5'-O-dimethylmyricetin
S-adenosyl-L-homocysteine + 3,3',5'-O-trimethylmyricetin
-
18% activity compared to myricetin
-
-
?
S-adenosyl-L-methionine + 3'-O-methylmyricetin
S-adenosyl-L-homocysteine + 3,3'-O-dimethylmyricetin
S-adenosyl-L-methionine + 3,5,7,3',4'-pentahydroxyflavone
S-adenosyl-L-homocysteine + 3-methoxy-5,7,3',4'-tetrahydroxyflavone
S-adenosyl-L-methionine + 3-O-methylquercetin
S-adenosyl-L-homocysteine + ?
-
3% activity compared to myricetin
-
-
?
S-adenosyl-L-methionine + 4'-O-methylkaempferol
S-adenosyl-L-homocysteine + 3,4'-O-dimethylkaempferol
-
11% activity compared to myricetin
-
-
?
S-adenosyl-L-methionine + 7-methylquercetin
?
-
i.e. rhamnetin, 30% of the activity with quercetin
-
-
?
S-adenosyl-L-methionine + 7-O-methylmyricetin
S-adenosyl-L-homocysteine + 3,7-O-dimethylquercetin
-
104% activity compared to myricetin
-
-
?
S-adenosyl-L-methionine + galangin
S-adenosyl-L-homocysteine + ?
-
60% of the activity with quercetin
-
-
?
S-adenosyl-L-methionine + isorhamnetin
S-adenosyl-L-homocysteine + ?
-
88% of the activity with quercetin
-
-
?
S-adenosyl-L-methionine + kaempferol
S-adenosyl-L-homocysteine + 3-O-methyl-kaempferol
-
93% of the activity with quercetin
-
-
?
S-adenosyl-L-methionine + kaempferol
S-adenosyl-L-homocysteine + 3-O-methylkaempferol
-
53% activity compared to myricetin
-
-
?
S-adenosyl-L-methionine + myricetin
S-adenosyl-L-homocysteine + 3-O-methyl-myricetin
-
46% of the activity with quercetin
-
-
?
S-adenosyl-L-methionine + myricetin
S-adenosyl-L-homocysteine + 3-O-methylmyricetin
-
100% activity
-
-
?
S-adenosyl-L-methionine + myricetin
S-adenosyl-L-homocysteine + ?
-
-
-
?
S-adenosyl-L-methionine + quercetin
S-adenosyl-L-homocysteine + 3'-methoxyquercetin
-
-
-
?
S-adenosyl-L-methionine + quercetin
S-adenosyl-L-homocysteine + 3-methoxyquercetin
-
-
-
?
S-adenosyl-L-methionine + quercetin
S-adenosyl-L-homocysteine + 3-O-methyl-quercetin
-
-
-
-
?
S-adenosyl-L-methionine + quercetin
S-adenosyl-L-homocysteine + 3-O-methylquercetin
-
121% activity compared to myricetin
-
-
?
S-adenosyl-L-methionine + quercetin
S-adenosyl-L-homocysteine + 5-methoxyquercetin
-
-
-
?
S-adenosyl-L-methionine + quercetin
S-adenosyl-L-homocysteine + 7-methoxyquercetin
-
-
-
?
S-adenosyl-L-methionine + tamarixetin
S-adenosyl-L-homocysteine + ?
-
85% of the activity with quercetin
-
-
?
additional information
?
-
S-adenosyl-L-methionine + 3'-O-methylmyricetin
S-adenosyl-L-homocysteine + 3,3'-O-dimethylmyricetin
-
64% activity compared to myricetin
-
-
?
S-adenosyl-L-methionine + 3'-O-methylmyricetin
S-adenosyl-L-homocysteine + 3,3'-O-dimethylmyricetin
-
i.e. laricitrin, 43% activity compared to myricetin
-
-
?
S-adenosyl-L-methionine + 3,5,7,3',4'-pentahydroxyflavone
S-adenosyl-L-homocysteine + 3-methoxy-5,7,3',4'-tetrahydroxyflavone
-
-
-
?
S-adenosyl-L-methionine + 3,5,7,3',4'-pentahydroxyflavone
S-adenosyl-L-homocysteine + 3-methoxy-5,7,3',4'-tetrahydroxyflavone
flavonol quercetin is the preferred substrate
-
-
?
S-adenosyl-L-methionine + 3,5,7,3',4'-pentahydroxyflavone
S-adenosyl-L-homocysteine + 3-methoxy-5,7,3',4'-tetrahydroxyflavone
involved in lignin biosynthesis
-
-
?
S-adenosyl-L-methionine + 3,5,7,3',4'-pentahydroxyflavone
S-adenosyl-L-homocysteine + 3-methoxy-5,7,3',4'-tetrahydroxyflavone
-
-
-
-
?
S-adenosyl-L-methionine + 3,5,7,3',4'-pentahydroxyflavone
S-adenosyl-L-homocysteine + 3-methoxy-5,7,3',4'-tetrahydroxyflavone
-
-
3-O-methylquercetin
?
S-adenosyl-L-methionine + 3,5,7,3',4'-pentahydroxyflavone
S-adenosyl-L-homocysteine + 3-methoxy-5,7,3',4'-tetrahydroxyflavone
-
enzymatic synthesis of polymethylated flavonol glucosides
-
-
?
S-adenosyl-L-methionine + 3,5,7,3',4'-pentahydroxyflavone
S-adenosyl-L-homocysteine + 3-methoxy-5,7,3',4'-tetrahydroxyflavone
-
involved in metabolism of secondary plant products, especially formation of lignin and detoxification of flavonoids
3-O-methylquercetin
?
S-adenosyl-L-methionine + 3,5,7,3',4'-pentahydroxyflavone
S-adenosyl-L-homocysteine + 3-methoxy-5,7,3',4'-tetrahydroxyflavone
Citrofortunella microcarpa
-
-
-
?
S-adenosyl-L-methionine + 3,5,7,3',4'-pentahydroxyflavone
S-adenosyl-L-homocysteine + 3-methoxy-5,7,3',4'-tetrahydroxyflavone
-
-
-
?
additional information
?
-
the flavonoid-O-methyltransferase from Citrus depressa has a broad substrate specificity and regioselectivity. Isozyme CdFOMT5 exhibits O-methyltransferase activity for quercetin, naringenin, (-)-epicatechin, and equol using S-adenosyl-L-methionine (SAM) as a methyl donor. The recombinant CdFOMT5 CdFOMT5 can catalyze the O-methylation of at least three hydroxyl groups of quercetin, and di- or tri-O-methylated quercetin products are obtained by this enzymatic reaction. CdFMOT5 prefers flavonol (3-hydroxyflavone) to other flavonoid structures. Thus, substrate structure, especially the C-ring in flavonoids, may strongly affect the substrate preference, including regioselectivity, of CdFOMT5
-
-
-
additional information
?
-
-
the flavonoid-O-methyltransferase from Citrus depressa has a broad substrate specificity and regioselectivity. Isozyme CdFOMT5 exhibits O-methyltransferase activity for quercetin, naringenin, (-)-epicatechin, and equol using S-adenosyl-L-methionine (SAM) as a methyl donor. The recombinant CdFOMT5 CdFOMT5 can catalyze the O-methylation of at least three hydroxyl groups of quercetin, and di- or tri-O-methylated quercetin products are obtained by this enzymatic reaction. CdFMOT5 prefers flavonol (3-hydroxyflavone) to other flavonoid structures. Thus, substrate structure, especially the C-ring in flavonoids, may strongly affect the substrate preference, including regioselectivity, of CdFOMT5
-
-
-
additional information
?
-
-
the enzyme does not methylate 3',4',5'-O-trimethylmyricetin, 3,7,3',4'-O-methylquercetin, 3-methylquercetin, and 3,7,4'-O-trimethylkaempferol. The enzyme transfers a methyl group only to the 3-position hydroxyl
-
-
?
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4 S-adenosyl-L-methionine + quercetin
4 S-adenosyl-L-homocysteine + 3,3',5,7-tetramethoxyquercetin
whole-cell biocatalysis using CdFOMT5 expressed in Escherichia coli cells is performed using quercetin as a substrate, and 3,3',5,7-tetramethylated quercetin is obtained as the final product
-
-
?
S-adenosyl-L-methionine + 3,5,7,3',4'-pentahydroxyflavone
S-adenosyl-L-homocysteine + 3-methoxy-5,7,3',4'-tetrahydroxyflavone
additional information
?
-
-
the enzyme does not methylate 3',4',5'-O-trimethylmyricetin, 3,7,3',4'-O-methylquercetin, 3-methylquercetin, and 3,7,4'-O-trimethylkaempferol. The enzyme transfers a methyl group only to the 3-position hydroxyl
-
-
?
S-adenosyl-L-methionine + 3,5,7,3',4'-pentahydroxyflavone
S-adenosyl-L-homocysteine + 3-methoxy-5,7,3',4'-tetrahydroxyflavone
-
-
-
?
S-adenosyl-L-methionine + 3,5,7,3',4'-pentahydroxyflavone
S-adenosyl-L-homocysteine + 3-methoxy-5,7,3',4'-tetrahydroxyflavone
involved in lignin biosynthesis
-
-
?
S-adenosyl-L-methionine + 3,5,7,3',4'-pentahydroxyflavone
S-adenosyl-L-homocysteine + 3-methoxy-5,7,3',4'-tetrahydroxyflavone
-
-
-
-
?
S-adenosyl-L-methionine + 3,5,7,3',4'-pentahydroxyflavone
S-adenosyl-L-homocysteine + 3-methoxy-5,7,3',4'-tetrahydroxyflavone
-
-
3-O-methylquercetin
?
S-adenosyl-L-methionine + 3,5,7,3',4'-pentahydroxyflavone
S-adenosyl-L-homocysteine + 3-methoxy-5,7,3',4'-tetrahydroxyflavone
-
enzymatic synthesis of polymethylated flavonol glucosides
-
-
?
S-adenosyl-L-methionine + 3,5,7,3',4'-pentahydroxyflavone
S-adenosyl-L-homocysteine + 3-methoxy-5,7,3',4'-tetrahydroxyflavone
-
involved in metabolism of secondary plant products, especially formation of lignin and detoxification of flavonoids
3-O-methylquercetin
?
S-adenosyl-L-methionine + 3,5,7,3',4'-pentahydroxyflavone
S-adenosyl-L-homocysteine + 3-methoxy-5,7,3',4'-tetrahydroxyflavone
Citrofortunella microcarpa
-
-
-
?
S-adenosyl-L-methionine + 3,5,7,3',4'-pentahydroxyflavone
S-adenosyl-L-homocysteine + 3-methoxy-5,7,3',4'-tetrahydroxyflavone
-
-
-
?
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A0A151UIW0_CAJCA
355
0
39356
TrEMBL
Secretory Pathway (Reliability: 4)
A0A151R8J6_CAJCA
377
0
41530
TrEMBL
other Location (Reliability: 1)
A0A151REM7_CAJCA
101
0
11826
TrEMBL
other Location (Reliability: 3)
A0A151U8I9_CAJCA
347
0
38730
TrEMBL
other Location (Reliability: 1)
A0A151TCK3_CAJCA
356
0
40106
TrEMBL
other Location (Reliability: 3)
A0A0B2QH47_GLYSO
232
0
25632
TrEMBL
other Location (Reliability: 3)
A0A2I0B4R0_9ASPA
165
0
18107
TrEMBL
other Location (Reliability: 5)
A0A0B2NPP8_GLYSO
370
0
41126
TrEMBL
other Location (Reliability: 1)
A0A5B7BI16_DAVIN
393
0
43308
TrEMBL
other Location (Reliability: 5)
A0A5B7BL78_DAVIN
364
0
40142
TrEMBL
other Location (Reliability: 1)
A0A151UFX0_CAJCA
97
0
11183
TrEMBL
other Location (Reliability: 4)
A0A151SVJ8_CAJCA
352
0
39819
TrEMBL
other Location (Reliability: 4)
A0A0B2PXG7_GLYSO
96
0
10390
TrEMBL
other Location (Reliability: 2)
A0A2I0AF71_9ASPA
319
0
33899
TrEMBL
Chloroplast (Reliability: 3)
A0A2I0B3Z8_9ASPA
160
1
17936
TrEMBL
other Location (Reliability: 2)
A0A151RL23_CAJCA
355
0
39466
TrEMBL
other Location (Reliability: 2)
A0A5B6ZZK7_DAVIN
140
0
14951
TrEMBL
other Location (Reliability: 5)
U5QFM0_9CYAN
332
0
36292
TrEMBL
-
A0A0B2PV24_GLYSO
358
0
40048
TrEMBL
other Location (Reliability: 2)
A0A151RAZ7_CAJCA
347
0
38651
TrEMBL
other Location (Reliability: 2)
A0A151S672_CAJCA
356
0
40725
TrEMBL
other Location (Reliability: 2)
A0A151TCK0_CAJCA
356
0
39941
TrEMBL
other Location (Reliability: 3)
A0A2H9ZSA4_9ASPA
385
0
42531
TrEMBL
other Location (Reliability: 1)
A0A151SVE2_CAJCA
358
0
40472
TrEMBL
other Location (Reliability: 2)
A0A0B2Q1N4_GLYSO
340
0
37776
TrEMBL
Secretory Pathway (Reliability: 3)
A0A151R701_CAJCA
339
0
38094
TrEMBL
other Location (Reliability: 2)
A0A0B2SRI5_GLYSO
337
0
37734
TrEMBL
other Location (Reliability: 3)
B0EKN1_ENTDS
Entamoeba dispar (strain ATCC PRA-260 / SAW760)
237
0
27598
TrEMBL
other Location (Reliability: 1)
A0A151QNN8_CAJCA
354
0
39462
TrEMBL
other Location (Reliability: 2)
K9XAK2_9CHRO
351
0
38591
TrEMBL
-
A0A0B2RF65_GLYSO
369
0
41571
TrEMBL
other Location (Reliability: 1)
A0A151QV14_CAJCA
73
0
7834
TrEMBL
Secretory Pathway (Reliability: 3)
A0A5B6ZY86_DAVIN
375
0
41026
TrEMBL
other Location (Reliability: 1)
A0A151TCI8_CAJCA
302
0
33818
TrEMBL
other Location (Reliability: 3)
H6L4R0_SAPGL
Saprospira grandis (strain Lewin)
323
0
36459
TrEMBL
-
A0A151QPK0_CAJCA
213
0
24150
TrEMBL
other Location (Reliability: 2)
A0A0B2SPY8_GLYSO
192
0
21180
TrEMBL
other Location (Reliability: 4)
A0A151SVG0_CAJCA
352
0
39792
TrEMBL
other Location (Reliability: 4)
A0A151SY09_CAJCA
354
0
39515
TrEMBL
other Location (Reliability: 2)
A0A5B7BKW7_DAVIN
354
0
39463
TrEMBL
other Location (Reliability: 4)
A0A2I0AJP8_9ASPA
537
0
58880
TrEMBL
Chloroplast (Reliability: 2)
A0A151RL45_CAJCA
198
0
22794
TrEMBL
Mitochondrion (Reliability: 5)
A0A151QUU7_CAJCA
339
0
37631
TrEMBL
Secretory Pathway (Reliability: 3)
A0A151QLB4_CAJCA
187
0
21213
TrEMBL
Secretory Pathway (Reliability: 5)
A0A151QS26_CAJCA
363
0
39656
TrEMBL
other Location (Reliability: 2)
A0A2I0AV56_9ASPA
384
0
41853
TrEMBL
other Location (Reliability: 4)
A0A151R8F1_CAJCA
382
0
41948
TrEMBL
Secretory Pathway (Reliability: 4)
A0A151TY80_CAJCA
366
0
40247
TrEMBL
other Location (Reliability: 1)
A0A151TYC1_CAJCA
332
0
36858
TrEMBL
other Location (Reliability: 4)
A0A0B2QE35_GLYSO
365
0
39969
TrEMBL
other Location (Reliability: 2)
A0A151ST55_CAJCA
253
0
28142
TrEMBL
other Location (Reliability: 3)
A0A151TCG4_CAJCA
348
0
38906
TrEMBL
other Location (Reliability: 2)
A0A151TCJ0_CAJCA
356
0
40078
TrEMBL
other Location (Reliability: 5)
A0A2I0B3Q1_9ASPA
352
0
38127
TrEMBL
Secretory Pathway (Reliability: 4)
A0A125T1T5_9ROSI
353
0
38978
TrEMBL
-
OMT1_ORYSJ
368
0
39749
Swiss-Prot
-
OMT1_ARATH
363
0
39618
Swiss-Prot
Secretory Pathway (Reliability: 4)
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Ibrahim, R.K.; De Luca, V.
Polymethylated flavonol synthesis is catalysed by distinct O-methyltranferases
Naturwissenschaften
69
41-42
1982
Chrysosplenium americanum
-
brenda
De Luca, V.; Ibrahim, R.K.
Enzymatic synthesis of polymethylated flavonols in Chrysosplenium americanum. I. Partial purification and some properties of S-adenosyl-L-methionine:flavonol 3-, 6-, 7-, and 4-O-methyltransferases
Arch. Biochem. Biophys.
238
596-605
1985
Chrysosplenium americanum, Citrofortunella microcarpa
brenda
De Luca, V.; Ibrahim, R.K.
Enzymatic synthesis of polymethylated flavonols in Chrysosplenium americanum. II. Substrate interaction and product inhibition studies of flavonol 3-, 6-, and 4-O-methyltransferases
Arch. Biochem. Biophys.
238
606-618
1985
Chrysosplenium americanum
brenda
Khouri, H.E.; Ibrahim, R.K.
Resolution of five position-specific flavonoid O-methyltransferases by fast protein liquid chromatofocusing
J. Chromatogr.
407
291-297
1987
Chrysosplenium americanum
-
brenda
Muzac, I.; Wang, J.; Anzellotti, D.; Zhang, H.; Ibrahim, R.K.
Functional expression of an Arabidopsis cDNA clone encoding a flavonol 3'-O-methyltransferase and characterization of the gene product
Arch. Biochem. Biophys.
375
385-388
2000
Arabidopsis thaliana, Arabidopsis thaliana (Q9FK25), Chrysosplenium americanum
brenda
Huang, T.S.; Anzellotti, D.; Dedaldechamp, F.; Ibrahim, R.K.
Partial purification, kinetic analysis, and amino acid sequence information of a flavonol 3-O-methyltransferase from Serratula tinctoria
Plant Physiol.
134
1366-1376
2004
Serratula tinctoria
brenda
Zhou, S.; Sauve, R.; Thannhauser, T.W.
Proteome changes induced by aluminium stress in tomato roots
J. Exp. Bot.
60
1849-1857
2009
Solanum lycopersicum
brenda
Schmidt, A.; Li, C.; Daniel Jones, A.; Pichersky, E.
Characterization of a flavonol 3-O-methyltransferase in the trichomes of the wild tomato species Solanum habrochaites
Planta
236
839-849
2012
Solanum habrochaites
brenda
Liu, C.W.; Chang, T.S.; Hsu, Y.K.; Wang, A.Z.; Yen, H.C.; Wu, Y.P.; Wang, C.S.; Lai, C.C.
Comparative proteomic analysis of early salt stress responsive proteins in roots and leaves of rice
Proteomics
14
1759-1775
2014
Oryza sativa (Q6ZD89), Oryza sativa
brenda
Itoh, N.; Iwata, C.; Toda, H.
Molecular cloning and characterization of a flavonoid-O-methyltransferase with broad substrate specificity and regioselectivity from Citrus depressa
BMC Plant Biol.
16
180
2016
Citrus depressa (A0A125T1T5), Citrus depressa
brenda