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S-adenosyl-L-methionine + 16S rRNA
S-adenosyl-L-homocysteine + 16S rRNA containing thymine
S-adenosyl-L-methionine + DNA analog of the T-stem/loop domain of yeast tRNAPhe
S-adenosyl-L-homocysteine + DNA analog of the T-stem/loop domain of yeast tRNAPhe containing thymine
-
DNA analog of the T-stem/loop domain of yeast tRNAPhe composed of d(A,U,G,C) is also a substrate, slow methylation of tDNAPheT
-
?
S-adenosyl-L-methionine + tRNA containing uridine at position 54
S-adenosyl-L-homocysteine + tRNA containing ribothymidine at position 54
S-adenosyl-L-methionine + uracil54 in 11mer of the T-arm of E. coli tRNA1-Val
S-adenosyl-L-homocysteine + 5-methyluracil54 in 11mer of the T-arm of E. coli tRNA1-Val
-
-
-
-
?
S-adenosyl-L-methionine + uracil54 in 17mer of the T-arm of E. coli tRNA1-Val
S-adenosyl-L-homocysteine + 5-methyluracil54 in 17mer of the T-arm of E. coli tRNA1-Val
-
-
-
-
?
S-adenosyl-L-methionine + uracil54 in methyl deficient tRNA
?
S-adenosyl-L-methionine + uracil54 in methyl deficient tRNA from E. coli
?
-
-
-
-
?
S-adenosyl-L-methionine + uracil54 in tRNA
S-adenosyl-L-homocysteine + 5-methyluracil54 in tRNA
-
-
-
?
S-adenosyl-L-methionine + uracil54 in unmodified E. coli tRNA1-Val
S-adenosyl-L-homocysteine + 5-methyluracil54 in unmodified E. coli tRNA1-Val
-
-
-
-
?
S-adenosyl-L-methionine + uracil54 in unmodified T-stem/loop domain of yeast tRNA-Phe
S-adenosyl-L-homocysteine + 5-methyluracil54 in unmodified T-stem/loop domain of yeast tRNA-Phe
-
-
-
-
?
S-adenosyl-L-methionine + uracil54 in unmodified yeast tRNA-Phe TPsiC-stem and loop heptadecamer
S-adenosyl-L-homocysteine + 5-methyluracil54 in unmodified yeast tRNA-Phe TPsiC-stem and loop heptadecamer
S-adenosyl-L-methionine + uracil54 in wheat germ tRNA1-Gly
S-adenosyl-L-homocysteine + 5-methyluracil54 in in wheat germ tRNA1-Gly
S-adenosyl-L-methionine + uracil54 in yeast tRNA-Phe
S-adenosyl-L-homocysteine + 5-methyluracil54 in yeast tRNA-Phe
S-adenosyl-L-methionine + uracil54 in yeast tRNA1-Phe
S-adenosyl-L-homocysteine + 5-methyluracil54 in yeast tRNA1-Phe
S-adenosyl-L-methionine + uridine54 in tRNA
S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA
additional information
?
-
S-adenosyl-L-methionine + 16S rRNA
S-adenosyl-L-homocysteine + 16S rRNA containing thymine
-
methyl group acceptor: E. coli 16S rRNA, site of methylation is U788 which is within a 9-base loop of a predicted stem-loop structure, mechanism is proposed to involve formation of a covalent, reversible Michael adduct with the target U788, poor substrate: methylation of 16S rRNA 40fold slower than of tRNAPhe
in vitro product m5U is not normally found in wild-type 16S rRNA, U788 is not highly methylated in vivo, 1 mol m5U per mol of 16S rRNA
?
S-adenosyl-L-methionine + 16S rRNA
S-adenosyl-L-homocysteine + 16S rRNA containing thymine
-
unmodified E. coli 16S rRNA can be methylated at U788
-
?
S-adenosyl-L-methionine + tRNA containing uridine at position 54
S-adenosyl-L-homocysteine + tRNA containing ribothymidine at position 54
-
-
-
-
?
S-adenosyl-L-methionine + tRNA containing uridine at position 54
S-adenosyl-L-homocysteine + tRNA containing ribothymidine at position 54
-
-
-
?
S-adenosyl-L-methionine + tRNA containing uridine at position 54
S-adenosyl-L-homocysteine + tRNA containing ribothymidine at position 54
-
-
-
-
?
S-adenosyl-L-methionine + tRNA containing uridine at position 54
S-adenosyl-L-homocysteine + tRNA containing ribothymidine at position 54
-
-
-
?
S-adenosyl-L-methionine + tRNA containing uridine at position 54
S-adenosyl-L-homocysteine + tRNA containing ribothymidine at position 54
-
PAB0719 enzyme or PabTrmU54 displays an identical specificity to eucaryontal homologue TrmA, as it catalyses the in vitro formation of m5U-54 in tRNA
-
-
?
S-adenosyl-L-methionine + tRNA containing uridine at position 54
S-adenosyl-L-homocysteine + tRNA containing ribothymidine at position 54
the enzyme purified under aerobic conditions is specific for tRNA but not rRNA, and specifically modifies the U54 position in the T-C loop of yeast tRNAAsp. A tRNA lacking both the D and anticodon stem-loops is recognized by PabTrmU54
The adenine ring of S-adenosyl-L-homocysteine makes van der Waals and hydrophobic interactions with residues S300 and Y278. The sugar ring of S-adenosyl-L-homocysteine stacks on P342 whereas its two oxygen atoms form H-bonds with the carboxylate group of D299. The terminal carboxylate of the homocysteine moiety is hydrogen bonded to the hydroxyl group of T283 and the amide side chain of Q252
-
?
S-adenosyl-L-methionine + uracil54 in methyl deficient tRNA
?
-
-
-
-
?
S-adenosyl-L-methionine + uracil54 in methyl deficient tRNA
?
-
-
-
-
?
S-adenosyl-L-methionine + uracil54 in unmodified yeast tRNA-Phe TPsiC-stem and loop heptadecamer
S-adenosyl-L-homocysteine + 5-methyluracil54 in unmodified yeast tRNA-Phe TPsiC-stem and loop heptadecamer
-
-
-
-
?
S-adenosyl-L-methionine + uracil54 in unmodified yeast tRNA-Phe TPsiC-stem and loop heptadecamer
S-adenosyl-L-homocysteine + 5-methyluracil54 in unmodified yeast tRNA-Phe TPsiC-stem and loop heptadecamer
-
-
-
-
?
S-adenosyl-L-methionine + uracil54 in wheat germ tRNA1-Gly
S-adenosyl-L-homocysteine + 5-methyluracil54 in in wheat germ tRNA1-Gly
-
-
-
-
?
S-adenosyl-L-methionine + uracil54 in wheat germ tRNA1-Gly
S-adenosyl-L-homocysteine + 5-methyluracil54 in in wheat germ tRNA1-Gly
-
-
-
-
?
S-adenosyl-L-methionine + uracil54 in wheat germ tRNA1-Gly
S-adenosyl-L-homocysteine + 5-methyluracil54 in in wheat germ tRNA1-Gly
-
-
-
-
?
S-adenosyl-L-methionine + uracil54 in yeast tRNA-Phe
S-adenosyl-L-homocysteine + 5-methyluracil54 in yeast tRNA-Phe
-
-
-
-
?
S-adenosyl-L-methionine + uracil54 in yeast tRNA-Phe
S-adenosyl-L-homocysteine + 5-methyluracil54 in yeast tRNA-Phe
-
-
-
-
?
S-adenosyl-L-methionine + uracil54 in yeast tRNA1-Phe
S-adenosyl-L-homocysteine + 5-methyluracil54 in yeast tRNA1-Phe
-
-
-
-
?
S-adenosyl-L-methionine + uracil54 in yeast tRNA1-Phe
S-adenosyl-L-homocysteine + 5-methyluracil54 in yeast tRNA1-Phe
-
-
-
-
r
S-adenosyl-L-methionine + uridine54 in tRNA
S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA
-
-
methylates 5-carbon of U in position 54 of tRNA, product is ribothymidine, which is invariant in the TPsiC loop
?
S-adenosyl-L-methionine + uridine54 in tRNA
S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA
-
methyl group acceptor: methyl-deficient tRNA from E. coli
-
?
S-adenosyl-L-methionine + uridine54 in tRNA
S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA
-
methyl group acceptor: methyl-deficient tRNA from E. coli
-
ir
S-adenosyl-L-methionine + uridine54 in tRNA
S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA
-
methyl group acceptor: methyl-deficient tRNA from E. coli
one ribothymidine per tRNA molecule, always at the 23rd nucleotide from the 3Â’ end
?
S-adenosyl-L-methionine + uridine54 in tRNA
S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA
-
methyl group acceptor: methyl-deficient tRNA from E. coli
methylates 5-carbon of U in position 54 of tRNA, product is ribothymidine, which is invariant in the TPsiC loop
?
S-adenosyl-L-methionine + uridine54 in tRNA
S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA
-
highly specific for tRNA and S-adenosyl-L-methionine, cannot be replaced by methionine plus ATP, formaldehyde plus tetrahydrofolic acid, methylcobalamin and methyltetrahydrofolic acid
-
ir
S-adenosyl-L-methionine + uridine54 in tRNA
S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA
-
tRNA recognition reside in the 3-dimensional structure of the 7-member T-loop rather than in its primary structure, base composition of the T-stem is also unimportant, only U54 and C56 are essential for activity, tRNA binding causes a conformational change of tRNA to expose the modification site in the T-arm
-
?
S-adenosyl-L-methionine + uridine54 in tRNA
S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA
-
5-methylation of U in the TPsiC-loop
-
?
S-adenosyl-L-methionine + uridine54 in tRNA
S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA
-
enzyme transfers methyl group to uridine in position 54 of the TPsi-loop of all E. coli tRNA species, requires the TPsi-arm and, most specifically, the bases U54-U55-C56-Pu57-A58 for tRNA recognition
formation of 5-methyluridine
?
S-adenosyl-L-methionine + uridine54 in tRNA
S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA
-
S-adenosyl-L-methionine/S-adenosyl-L-homocysteine binding site, terminal NH2-group of the amino acid moiety is nessecary for activity
-
?
S-adenosyl-L-methionine + uridine54 in tRNA
S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA
-
extend of methylation is directly proportional to the amount of tRNA added, 0.5% of nucleotide residues are methylated
-
ir
S-adenosyl-L-methionine + uridine54 in tRNA
S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA
-
methyl group acceptor: thymine-deficient tRNA from E. coli trmA-mutant
-
?
S-adenosyl-L-methionine + uridine54 in tRNA
S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA
-
methyl group acceptors: methyl-deficient and T-deficient E. coli tRNA, enzyme recognizes and methylates TSL: a minimal unmodified yeast tRNAPhe TPsiC-stem and loop heptadecamer, composed of a 5 bp stem and seven-membered loop, recognition determinants are contained within the sequence, modification and secondary structure of TSL, enzyme is sensitive to conformational perturbations at or adjacent to the methylation site, positions 54 and 55
formation of 5-methyluridine in position 54 of tRNA, in the TPsiC sequence in loop IV
?
S-adenosyl-L-methionine + uridine54 in tRNA
S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA
-
methyl group acceptors: small RNA oligomers corresponding to the T-arm of tRNA, 17-oligomer
formation of 5-methyluridine in position 54 of tRNA, in the TPsiC sequence in loop IV
?
S-adenosyl-L-methionine + uridine54 in tRNA
S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA
-
methyl group acceptors: E. coli tRNA1Val, 17-oligomer and 11-oligomer of the T-arm of tRNA1Val, essential sequence for tRNA binding: within the T-arm, composed of 7-base T-loop and 5-base pair stem, includes position 52 of T-stem, the T-loop and extends to the 3'-acceptor end, methylation site at U54
-
?
S-adenosyl-L-methionine + uridine54 in tRNA
S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA
-
tRNAs from wheat germ are the best substrates
formation of 5-methyluridine
?
S-adenosyl-L-methionine + uridine54 in tRNA
S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA
-
substrate consensus sequence for the T-arm of tRNA consists of a 2-5 base-pair stem and a 7-base loop
-
?
S-adenosyl-L-methionine + uridine54 in tRNA
S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA
-
substrate consensus sequence for the T-arm of tRNA consists of a 2-5 base-pair stem and a 7-base loop
-
?
S-adenosyl-L-methionine + uridine54 in tRNA
S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA
-
methyl group acceptor: tRNA from Micrococcus lysodeikticus
-
?
S-adenosyl-L-methionine + uridine54 in tRNA
S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA
-
methyl group acceptor: m5U-deficient tRNA from E. coli GB1-5-39
-
?
S-adenosyl-L-methionine + uridine54 in tRNA
S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA
-
methyl group acceptor: m5U-deficient tRNA from E. coli GB1-5-39
formation of 5-methyluridine in position 54 of tRNA, in the TPsiC sequence in loop IV
?
S-adenosyl-L-methionine + uridine54 in tRNA
S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA
-
methyl group acceptor: m5U-deficient tRNA from E. coli GB1-5-39
methylates 5-carbon of U in position 54 of tRNA, product is ribothymidine, which is invariant in the TPsiC loop
?
S-adenosyl-L-methionine + uridine54 in tRNA
S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA
-
methyl group acceptor: unmodified yeast tRNAPhe
-
?
S-adenosyl-L-methionine + uridine54 in tRNA
S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA
-
methyl group acceptor: unmodified yeast tRNAPhe
-
?
S-adenosyl-L-methionine + uridine54 in tRNA
S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA
-
methyl group acceptor: unmodified yeast tRNAPhe
-
?
S-adenosyl-L-methionine + uridine54 in tRNA
S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA
-
methyl group acceptor: unmodified yeast tRNAPhe
-
?
S-adenosyl-L-methionine + uridine54 in tRNA
S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA
-
methyl group acceptors: unmodified T-stem/loop domain of yeast tRNAPhe, methyl-deficient tRNA from E. coli C6 rel met- cys-, riboT-deficient tRNA from E. coli TrmA- is a specific substrate for U54 methylation, enzyme recognizes almost all tRNAs
-
?
S-adenosyl-L-methionine + uridine54 in tRNA
S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA
-
methyl group acceptor: tRNA from E. coli
-
?
S-adenosyl-L-methionine + uridine54 in tRNA
S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA
-
methyl group acceptor: tRNA from E. coli
formation of 5-methyluridine in position 54 of tRNA, in the TPsiC sequence in loop IV
?
S-adenosyl-L-methionine + uridine54 in tRNA
S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA
-
methyl group acceptor: tRNA from E. coli
formation of 5-methyluridine
?
S-adenosyl-L-methionine + uridine54 in tRNA
S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA
-
methyl group acceptors: E. coli tRNA1Val, 17-mer analog of the T-arm from tRNA1Val
-
?
S-adenosyl-L-methionine + uridine54 in tRNA
S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA
-
high substrate specificity, methyl group acceptors: wheat germ tRNA1Gly, tRNA2Gly, E. coli tRNA1Ala
formation of 5-methyluridine
?
S-adenosyl-L-methionine + uridine54 in tRNA
S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA
-
methyl group acceptors: a variety of tRNA substrates including prokaryotic, eukaryotic, mitochondrial and chloroplastic tRNAs, wheat germ tRNA1Gly, bovine liver tRNAVal, Dictyostelium discoideum vegetative stage mitochondrial tRNA and rabbit liver tRNA, methylates E. coli tRNAs at a specific U residue 23 nucleotides from the 3Â’ end
formation of 5-methyluridine
?
S-adenosyl-L-methionine + uridine54 in tRNA
S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA
-
methyl group acceptors: E. coli tRNAPhe, tRNAVal
-
?
S-adenosyl-L-methionine + uridine54 in tRNA
S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA
-
tRNA recognition
-
?
S-adenosyl-L-methionine + uridine54 in tRNA
S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA
-
tRNA recognition
methylates 5-carbon of U in position 54 of tRNA, product is ribothymidine, which is invariant in the TPsiC loop
?
S-adenosyl-L-methionine + uridine54 in tRNA
S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA
-
enzyme is normally tightly associated with the tRNA
formation of 5-methyluridine in position 54 of tRNA, in the TPsiC sequence in loop IV
?
S-adenosyl-L-methionine + uridine54 in tRNA
S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA
-
tRNA and S-adenosyl-L-methionine have separate and independent binding sites
one ribothymidine per tRNA molecule, always at the 23rd nucleotide from the 3Â’ end
?
S-adenosyl-L-methionine + uridine54 in tRNA
S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA
-
methylation of U54
-
?
S-adenosyl-L-methionine + uridine54 in tRNA
S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA
-
methylates U54 in the TPsiC-loop of tRNA
-
?
S-adenosyl-L-methionine + uridine54 in tRNA
S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA
-
methylates U54 in the TPsiC-loop of tRNA
formation of 5-methyluridine in position 54 of tRNA, in the TPsiC sequence in loop IV
?
S-adenosyl-L-methionine + uridine54 in tRNA
S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA
-
methylates U54 in the TPsiC-loop of tRNA
formation of 5-methyluridine in position 54 of tRNA, in the TPsiC sequence in loop IV
?
S-adenosyl-L-methionine + uridine54 in tRNA
S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA
-
methyl group acceptor: thymine-deficient tRNA from E. coli
-
?
S-adenosyl-L-methionine + uridine54 in tRNA
S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA
-
in vivo present both as 42 kDa polypeptide and as covalent TrmA/RNA complex, to equal amounts, both with methylating activity, in vivo the m5U54 modification confers a small selective growth advantage, two different catalytic activities: synthesis of m5U54 and an unknown essential function, may be associated with binding RNA, either during a maturation process of the rRNA and/or tRNA or as a regulatory device during the assembly and maturation of the translational apparatus such as an RNA chaperone
-
?
S-adenosyl-L-methionine + uridine54 in tRNA
S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA
-
specific reaction in maturation of tRNA
ribothymidine is the most common methylated nucleoside found in tRNA, it occurs in all tRNAs of the organism
?
S-adenosyl-L-methionine + uridine54 in tRNA
S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA
-
tRNA modifying enzyme
-
?
S-adenosyl-L-methionine + uridine54 in tRNA
S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA
-
tRNA modifying enzyme
-
?
S-adenosyl-L-methionine + uridine54 in tRNA
S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA
-
tRNA modifying enzyme
-
?
S-adenosyl-L-methionine + uridine54 in tRNA
S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA
-
tRNA modifying enzyme
-
?
S-adenosyl-L-methionine + uridine54 in tRNA
S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA
-
tRNA modifying enzyme
-
?
S-adenosyl-L-methionine + uridine54 in tRNA
S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA
-
tRNA modifying enzyme
-
?
S-adenosyl-L-methionine + uridine54 in tRNA
S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA
-
enzyme-tRNA complexes may exist as dihydrouridine intermediates and may serve as reservoirs of RUMT-tRNA complexes primed for methylation
-
?
S-adenosyl-L-methionine + uridine54 in tRNA
S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA
-
involved in biosynthesis of all tRNA chains, association of enzyme to tRNA may be of regulatory significance
-
?
S-adenosyl-L-methionine + uridine54 in tRNA
S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA
-
specific inhibition of enzyme by ethionine in vivo
-
?
S-adenosyl-L-methionine + uridine54 in tRNA
S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA
-
primary function of nucleoside modification within the tRNA anticodon loop is to negate potential canonical base pairs that would close the loop and, thereby, hinder codon binding
-
?
S-adenosyl-L-methionine + uridine54 in tRNA
S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA
-
enzyme transfers methyl group to uridine in position 54 of the TPsi-loop of all E. coli tRNA species, requires the TPsi-arm and, most specifically, the bases U54-U55-C56-Pu57-A58 for tRNA recognition
formation of 5-methyluridine
?
S-adenosyl-L-methionine + uridine54 in tRNA
S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA
-
methyl group acceptor: tRNA from E. coli
formation of 5-methyluridine
?
S-adenosyl-L-methionine + uridine54 in tRNA
S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA
-
in vivo present both as 42 kDa polypeptide and as covalent TrmA/RNA complex, to equal amounts, both with methylating activity, in vivo the m5U54 modification confers a small selective growth advantage, two different catalytic activities: synthesis of m5U54 and an unknown essential function, may be associated with binding RNA, either during a maturation process of the rRNA and/or tRNA or as a regulatory device during the assembly and maturation of the translational apparatus such as an RNA chaperone
-
?
S-adenosyl-L-methionine + uridine54 in tRNA
S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA
-
methyl group acceptor: m5U-deficient tRNA from E. coli GB1-5-39
formation of 5-methyluridine in position 54 of tRNA, in the TPsiC sequence in loop IV
?
S-adenosyl-L-methionine + uridine54 in tRNA
S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA
-
enzyme is normally tightly associated with the tRNA
formation of 5-methyluridine in position 54 of tRNA, in the TPsiC sequence in loop IV
?
S-adenosyl-L-methionine + uridine54 in tRNA
S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA
-
tRNA modifying enzyme
-
?
S-adenosyl-L-methionine + uridine54 in tRNA
S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA
-
involved in biosynthesis of all tRNA chains, association of enzyme to tRNA may be of regulatory significance
-
?
S-adenosyl-L-methionine + uridine54 in tRNA
S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA
-
5-methylation of U in the TPsiC-loop
-
?
S-adenosyl-L-methionine + uridine54 in tRNA
S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA
-
methyl group acceptor: m5U-deficient tRNA from E. coli GB1-5-39
-
?
S-adenosyl-L-methionine + uridine54 in tRNA
S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA
-
tRNA modifying enzyme
-
?
S-adenosyl-L-methionine + uridine54 in tRNA
S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA
-
-
methylates 5-carbon of U in position 54 of tRNA, product is ribothymidine, which is invariant in the TPsiC loop
?
S-adenosyl-L-methionine + uridine54 in tRNA
S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA
-
methyl group acceptors: methyl-deficient and T-deficient E. coli tRNA, enzyme recognizes and methylates TSL: a minimal unmodified yeast tRNAPhe TPsiC-stem and loop heptadecamer, composed of a 5 bp stem and seven-membered loop, recognition determinants are contained within the sequence, modification and secondary structure of TSL, enzyme is sensitive to conformational perturbations at or adjacent to the methylation site, positions 54 and 55
formation of 5-methyluridine in position 54 of tRNA, in the TPsiC sequence in loop IV
?
S-adenosyl-L-methionine + uridine54 in tRNA
S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA
-
methylates U54 in the TPsiC-loop of tRNA
formation of 5-methyluridine in position 54 of tRNA, in the TPsiC sequence in loop IV
?
S-adenosyl-L-methionine + uridine54 in tRNA
S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA
-
tRNA modifying enzyme
-
?
S-adenosyl-L-methionine + uridine54 in tRNA
S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA
-
methyl group acceptor: methyl-deficient tRNA from E. coli
one ribothymidine per tRNA molecule, always at the 23rd nucleotide from the 3Â’ end
?
S-adenosyl-L-methionine + uridine54 in tRNA
S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA
-
tRNA and S-adenosyl-L-methionine have separate and independent binding sites
one ribothymidine per tRNA molecule, always at the 23rd nucleotide from the 3Â’ end
?
S-adenosyl-L-methionine + uridine54 in tRNA
S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA
-
specific reaction in maturation of tRNA
ribothymidine is the most common methylated nucleoside found in tRNA, it occurs in all tRNAs of the organism
?
S-adenosyl-L-methionine + uridine54 in tRNA
S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA
-
tRNAs from wheat germ are the best substrates
formation of 5-methyluridine
?
S-adenosyl-L-methionine + uridine54 in tRNA
S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA
-
methyl group acceptors: a variety of tRNA substrates including prokaryotic, eukaryotic, mitochondrial and chloroplastic tRNAs, wheat germ tRNA1Gly, bovine liver tRNAVal, Dictyostelium discoideum vegetative stage mitochondrial tRNA and rabbit liver tRNA, methylates E. coli tRNAs at a specific U residue 23 nucleotides from the 3Â’ end
formation of 5-methyluridine
?
S-adenosyl-L-methionine + uridine54 in tRNA
S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA
-
tRNA modifying enzyme
-
?
S-adenosyl-L-methionine + uridine54 in tRNA
S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA
-
high substrate specificity, methyl group acceptors: wheat germ tRNA1Gly, tRNA2Gly, E. coli tRNA1Ala
formation of 5-methyluridine
?
S-adenosyl-L-methionine + uridine54 in tRNA
S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA
-
tRNAs from wheat germ are the best substrates
formation of 5-methyluridine
?
S-adenosyl-L-methionine + uridine54 in tRNA
S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA
-
methyl group acceptors: a variety of tRNA substrates including prokaryotic, eukaryotic, mitochondrial and chloroplastic tRNAs, wheat germ tRNA1Gly, bovine liver tRNAVal, Dictyostelium discoideum vegetative stage mitochondrial tRNA and rabbit liver tRNA, methylates E. coli tRNAs at a specific U residue 23 nucleotides from the 3Â’ end
formation of 5-methyluridine
?
S-adenosyl-L-methionine + uridine54 in tRNA
S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA
-
tRNA modifying enzyme
-
?
S-adenosyl-L-methionine + uridine54 in tRNA
S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA
-
-
enzyme is responsible for the formation of 5-methyluridine at position 54 in tRNA
?
S-adenosyl-L-methionine + uridine54 in tRNA
S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA
-
methyl group acceptor: methyl-deficient tRNA from E. coli
-
?
S-adenosyl-L-methionine + uridine54 in tRNA
S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA
-
methyl group acceptor: yeast tRNA
nearly all tRNAs contain m5U54, initiators excluded
?
S-adenosyl-L-methionine + uridine54 in tRNA
S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA
-
tRNAs from wheat germ are the best substrates
thymine is 5-methyluracil, methylation to different extents and at different positions of tRNA by 3 or 4 isoforms
?
S-adenosyl-L-methionine + uridine54 in tRNA
S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA
-
methyl group acceptor: methyl-deficient tRNA from E. coli W6
thymine is 5-methyluracil, methylation to different extents and at different positions of tRNA by 3 or 4 isoforms
?
S-adenosyl-L-methionine + uridine54 in tRNA
S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA
-
methyl group acceptor: m5U-deficient tRNA from E. coli GB1-5-39
nearly all tRNAs contain m5U54, initiators excluded
?
S-adenosyl-L-methionine + uridine54 in tRNA
S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA
-
methyl group acceptor: wheat germ tRNA
thymine is 5-methyluracil, methylation to different extents and at different positions of tRNA by 3 or 4 isoforms
?
S-adenosyl-L-methionine + uridine54 in tRNA
S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA
-
methylation of U54
nearly all tRNAs contain m5U54, initiators excluded
?
S-adenosyl-L-methionine + uridine54 in tRNA
S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA
-
role per se in tRNA maturation, stabilization of tRNA, early event in tRNA maturation: intron-containing pre-tRNAs contain m5U
-
?
S-adenosyl-L-methionine + uridine54 in tRNA
S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA
-
methyl group acceptor: methyl-deficient tRNA from E. coli
-
?
S-adenosyl-L-methionine + uridine54 in tRNA
S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA
-
tRNAs from wheat germ are the best substrates
thymine is 5-methyluracil, methylation to different extents and at different positions of tRNA by 3 or 4 isoforms
?
S-adenosyl-L-methionine + uridine54 in tRNA
S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA
-
methyl group acceptor: methyl-deficient tRNA from E. coli W6
thymine is 5-methyluracil, methylation to different extents and at different positions of tRNA by 3 or 4 isoforms
?
S-adenosyl-L-methionine + uridine54 in tRNA
S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA
-
methyl group acceptor: wheat germ tRNA
thymine is 5-methyluracil, methylation to different extents and at different positions of tRNA by 3 or 4 isoforms
?
S-adenosyl-L-methionine + uridine54 in tRNA
S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA
-
-
-
?
additional information
?
-
TRMT2A commonly targets U54 of cytosolic tRNAs
-
-
-
additional information
?
-
-
TRMT2A commonly targets U54 of cytosolic tRNAs
-
-
-
additional information
?
-
the enzyme emerged through an ancient horizontal transfer of an RNA (uracil, C5)-methyltransferase-like gene from bacteria to archaea
-
-
?
additional information
?
-
structure-function analysis using mini-tRNA stem-loop, overview
-
-
?
additional information
?
-
-
recombinant PAB0719 is shown to function like TrmA from Escherichia coli and catalyzes m5U formation at position 54 in Pyrococcus abyssi tRNAs
-
-
?
additional information
additional information
-
-
tetrahydrofolate derivatives as methyl group donor in the formation of 5-methyluridine
-
-
?
additional information
additional information
-
-
tetrahydrofolate derivatives as methyl group donor in the formation of 5-methyluridine
-
-
?
additional information
additional information
-
-
tetrahydrofolate derivatives as methyl group donor in the formation of 5-methyluridine
-
-
?
additional information
additional information
-
-
-
-
-
?
additional information
additional information
-
-
-
-
-
?
additional information
additional information
-
-
not as methyl group acceptors: methyl-deficient DNA from E. coli, polyadenylic acid, polycytidylic acid, polyuridylic acid, polyinosinic acid, copolymers of adenylic acid and uridylic acid and of guanylic acid and cytidylic acid, tobacco mosaic viral RNA, turnip yellow mosaic viral RNA, RNA from bacteriophage f2, CMP, AMP, UMP, GMP, rRNA
-
-
?
additional information
additional information
-
-
enzyme also catalyzes exchange of tritium of [3H]-Ura-16S rRNA and protons of water
-
-
?
additional information
additional information
-
-
detailed mechanism, Cys-324 is the nucleophilic catalyst of enzyme, involves SN2 attack of C5 of U54 on the methyl group of AdoMet
-
-
?
additional information
additional information
-
-
poor acceptors: tRNAs from spinach chloroplasts, Dictyostelium discoideum vegetative stage, Bacillus subtilis and Anacystis nidulans, unfractionated E. coli tRNA
-
-
?
additional information
additional information
-
-
up to 50% of enzyme is covalently bound to various tRNA species, including several tRNAs at the 3Â’ end of 16S rRNA
-
-
?
additional information
additional information
-
-
enzyme is present as 42 kDa native protein or as 2 different covalent TrmA/RNA complexes, with TrmA as a 54 kDa and a 62 kDa polypeptide, respectively, RNA is a subset of undermodified tRNA species as well as the 3Â’ terminus of 16S rRNA, RNA does not affect enzyme activity
-
-
?
additional information
additional information
-
-
enzyme is present as 42 kDa native protein or as 48-57 kDa RNA-protein complex, RNA is not essential, but may increase enzyme activity
-
-
?
additional information
additional information
-
-
enzyme also catalyzes an S-adenosyl-L-methionine-independent exchange reaction between [5-3H]-Urd-labeled substrate tRNA and protons of water at 1% of the normal methylation reaction rate, mechanism
-
-
?
additional information
additional information
-
-
enzyme also catalyzes an S-adenosyl-L-methionine-independent exchange reaction between [5-3H]-Urd-labeled substrate tRNA and protons of water at 1% of the normal methylation reaction rate, mechanism
-
-
?
additional information
additional information
-
-
enzyme is present as 42 kDa native protein or as 2 different covalent TrmA/RNA complexes, with TrmA as a 54 kDa and a 62 kDa polypeptide, respectively, RNA is a subset of undermodified tRNA species as well as the 3Â’ terminus of 16S rRNA, RNA does not affect enzyme activity
-
-
?
additional information
additional information
-
-
enzyme is present as 42 kDa native protein or as 48-57 kDa RNA-protein complex, RNA is not essential, but may increase enzyme activity
-
-
?
additional information
additional information
-
-
enzyme also catalyzes an S-adenosyl-L-methionine-independent exchange reaction between [5-3H]-Urd-labeled substrate tRNA and protons of water at 1% of the normal methylation reaction rate, mechanism
-
-
?
additional information
additional information
-
-
-
-
-
?
additional information
additional information
-
-
poor acceptors: tRNAs from spinach chloroplasts, Dictyostelium discoideum vegetative stage, Bacillus subtilis and Anacystis nidulans, unfractionated E. coli tRNA
-
-
?
additional information
additional information
-
-
-
-
-
?
additional information
additional information
-
-
poor acceptors: tRNAs from spinach chloroplasts, Dictyostelium discoideum vegetative stage, Bacillus subtilis and Anacystis nidulans, unfractionated E. coli tRNA
-
-
?
additional information
additional information
-
-
3 isoenzymes with different substrate specificities, also some activity with rRNA from E. coli, may be due to a contamination
-
-
?
additional information
additional information
-
-
absence of TRM2 gene encoding enzyme in strains with mutant forms of tRNASerCGA decreases the stability of tRNASerCGA and induces lethality
-
-
?
additional information
additional information
-
-
3 isoenzymes with different substrate specificities, also some activity with rRNA from E. coli, may be due to a contamination
-
-
?
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Studies on microbial RNA. Fractionation of tRNA methylases from Saccharomyces cerevisiae
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9
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The enzymatic methylation of ribonucleic acid and deoxyribonucleic acid. III. Purification of soluble ribonucleic acid-methylating enzymes
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239
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36
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Studies on microbial RNA. Properties of tRNA methylases from Saccharomyces cerevisiae
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Escherichia coli tRNA (uracil-5-)-methyltransferase: Inhibition by analogues of adenosylhomocysteine
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24
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Isolation and characterization of m5U-methyltransferase from Escherichia coli
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255
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Bacterial tRNA methyltransferases
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59
190-203
1979
Bacillus cereus, Bacillus subtilis, Enterococcus faecalis, Escherichia coli, Escherichia coli MRE 600
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The tRNA-(m5U54)-methyltransferase of Escherichia coli is present in two forms in vivo, one of which is present as bound to tRNA and to a 3-end fragment of 16S rRNA
J. Biol. Chem.
268
1326-1331
1993
Escherichia coli, Escherichia coli 1100
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Kinetic studies of Escherichia coli transfer RNA (uracil-5-)-methyltransferase
Biochemistry
17
1068-1072
1978
Escherichia coli, Escherichia coli K12MO7
brenda
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Catalytic mechanism and inhibition of tRNA (uracil-5-)methyltransferase: Evidence for covalent catalysis
Biochemistry
26
8599-8606
1987
Escherichia coli, Escherichia coli GB-1-5-39 / pTN102
brenda
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The T-arm of tRNA is a substrate for tRNA (m5U54)-methyltransferase
Biochemistry
30
2999-3002
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Escherichia coli
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Purification of transfer RNA (m5U54)-methyltransferase from Escherichia coli. Association with RNA
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177
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Escherichia coli, Escherichia coli C600 / pTN106
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Stereochemistry of methyl transfer catalyzed by tRNA (m5U54)-methyltransferase--evidence for a single displacement mechanism
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19
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Escherichia coli
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Selective inhibition of uracil tRNA methylases of E. coli by ethionine
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5
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Escherichia coli
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Enzymatic mechanism of tRNA (m5U54)methyltransferase
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76
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Escherichia coli
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High-level expression and rapid purification of tRNA (m5U54)-methyltransferase
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5
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Escherichia coli, Escherichia coli GB-1-5-39 / pTN102
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24
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34
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1995
Escherichia coli
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In vitro methylation of Escherichia coli 16S rRNA by tRNA (m5U54)-methyltransferase
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33
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Escherichia coli
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Aminoacyl-tRNA synthetase and U54 methyltransferase recognize conformations of the yeast tRNAPhe anticodon and T stem/loop domain
Biochimie
76
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1994
Escherichia coli
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Modified constructs of the tRNA TPsiC domain to probe substrate conformational requirements of m1A58 and m5U54 tRNA methyltransferases
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28
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Recognition of the T-arm of tRNA by tRNA (m5U54)-methyltransferase is not sequence specific
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35
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1996
Escherichia coli
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Identification of the TRM2 gene encoding the tRNA(m5U54)methyltransferase of Saccharomyces cerevisiae
RNA
6
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2000
Saccharomyces cerevisiae
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Dual function of the tRNA(m5U54)methyltransferase in tRNA maturation
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8
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2002
Saccharomyces cerevisiae
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Amino acid residues of the Escherichia coli tRNA(m5U54)methyltransferase (TrmA) critical for stability, covalent binding of tRNA and enzymatic activity
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35
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Escherichia coli
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105
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17
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