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Synonyms
aimt1, chavicol o-methyltransferase, eugenol o-methyltransferase, rcomt1, (iso)eugenol o-methyltransferase, monolignol 4-o-methyltransferase, momt5, s-adenosyl-l-methionine:(iso)eugenol o-methyltransferase, isoeugenol-o-methyltransferase, eugenol-o-methyltransferase,
more
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S-adenosyl-L-methionine + 2-methoxy-4-methylphenol
S-adenosyl-L-homocysteine + 1,2-dimethoxy-4-methylbenzene
-
16.8% of the activity with eugenol
-
-
?
S-adenosyl-L-methionine + 2-methoxyphenol
S-adenosyl-L-homocysteine + ?
-
4% of the activity with eugenol
-
-
?
S-adenosyl-L-methionine + 5-methoxyeugenol
S-adenosyl-L-homocysteine + ?
-
about 18% of the activity with isoeugenol
-
-
?
S-adenosyl-L-methionine + caffeyl alcohol
S-adenosyl-L-homocysteine + 4-methoxycaffeyl alcohol
-
-
-
?
S-adenosyl-L-methionine + chavicol
S-adenosyl-L-homocysteine + estragol
24% of the activity with eugenol
-
-
?
S-adenosyl-L-methionine + coniferyl alcohol
S-adenosyl-L-homocysteine + 4-methoxyconiferyl alcohol
-
-
-
?
S-adenosyl-L-methionine + coumaryl alcohol
S-adenosyl-L-homocysteine + 4-methoxycoumaryl alcohol
-
-
-
?
S-adenosyl-L-methionine + dihydrochavicol
S-adenosyl-L-homocysteine + ?
-
about 23% of the activity with isoeugenol
-
-
?
S-adenosyl-L-methionine + dihydroeugenol
S-adenosyl-L-homocysteine + methyldihydroeugenol
-
about 50% of the activity with isoeugenol
-
-
?
S-adenosyl-L-methionine + eugenol
S-adenosyl-L-homocysteine + methyleugenol
S-adenosyl-L-methionine + guaiacol
S-adenosyl-L-homocysteine + 1,2-dimethoxybenzene
29.9% of the activity with eugenol
-
-
?
S-adenosyl-L-methionine + isoeugenol
S-adenosyl-L-homocysteine + isomethyleugenol
S-adenosyl-L-methionine + isoeugenol
S-adenosyl-L-homocysteine + methylisoeugenol
S-adenosyl-L-methionine + monolignol
S-adenosyl-L-homocysteine + 4-O-methylmonolignol
-
-
-
?
S-adenosyl-L-methionine + sinapyl alcohol
S-adenosyl-L-homocysteine + 4-methoxysinapyl alcohol
-
-
-
?
S-adenosyl-L-methionine + t-anol
S-adenosyl-L-homocysteine + t-anethole
-
methylation of the p-OH group. 45% of the rate with isoeugenol
-
-
?
S-adenosyl-L-methionine + trans-isoeugenol
S-adenosyl-L-homocysteine + (E)-2',3'-dimethoxy-1-propenylbenzene
-
-
-
?
S-adenosyl-L-methionine + zingerone
S-adenosyl-L-homocysteine + 4-(3,4-dimethoxyphenyl)-butan-2-one
-
26.8% of the activity with eugenol
-
-
?
additional information
?
-
S-adenosyl-L-methionine + eugenol
S-adenosyl-L-homocysteine + methyleugenol
-
-
?
S-adenosyl-L-methionine + eugenol
S-adenosyl-L-homocysteine + methyleugenol
-
-
?
S-adenosyl-L-methionine + eugenol
S-adenosyl-L-homocysteine + methyleugenol
-
-
-
-
?
S-adenosyl-L-methionine + eugenol
S-adenosyl-L-homocysteine + methyleugenol
-
the enzyme is involved in the production of the floral volatile compounds methyleugenol and methylisoeugenol
-
-
?
S-adenosyl-L-methionine + eugenol
S-adenosyl-L-homocysteine + methyleugenol
-
-
-
?
S-adenosyl-L-methionine + eugenol
S-adenosyl-L-homocysteine + methyleugenol
-
-
-
-
?
S-adenosyl-L-methionine + isoeugenol
S-adenosyl-L-homocysteine + isomethyleugenol
-
-
?
S-adenosyl-L-methionine + isoeugenol
S-adenosyl-L-homocysteine + isomethyleugenol
-
-
?
S-adenosyl-L-methionine + isoeugenol
S-adenosyl-L-homocysteine + isomethyleugenol
-
-
-
-
?
S-adenosyl-L-methionine + isoeugenol
S-adenosyl-L-homocysteine + isomethyleugenol
26% of the activity with eugenol
-
?
S-adenosyl-L-methionine + isoeugenol
S-adenosyl-L-homocysteine + isomethyleugenol
-
-
-
?
S-adenosyl-L-methionine + isoeugenol
S-adenosyl-L-homocysteine + methylisoeugenol
-
-
-
-
?
S-adenosyl-L-methionine + isoeugenol
S-adenosyl-L-homocysteine + methylisoeugenol
-
the enzyme is involved in the production of the floral volatile compounds methyleugenol and methylisoeugenol
-
-
?
S-adenosyl-L-methionine + isoeugenol
S-adenosyl-L-homocysteine + methylisoeugenol
-
methylation of the p-OH group
-
-
?
S-adenosyl-L-methionine + isoeugenol
S-adenosyl-L-homocysteine + methylisoeugenol
-
75% of the activity with eugenol
-
-
?
additional information
?
-
substrate specificities of engineered monolignol 4-O-methyltransferases, MOMT3, MOMT4, MOMT5, MOMT8, and MOMT9
-
-
-
additional information
?
-
-
no substrate: chavicol, eugenol, guaiacol, caffeic acid, 2-propylphenol
-
-
?
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0.0114 - 0.1985
coniferyl alcohol
0.0193 - 0.214
isoeugenol
0.005 - 0.0545
S-adenosyl-L-methionine
0.0147 - 0.1187
sinapyl alcohol
0.0114
coniferyl alcohol
pH 7.5, 30°C, recombinant enzyme mutant MOMT5
0.0161
coniferyl alcohol
pH 7.5, 30°C, recombinant enzyme mutant MOMT8
0.0161
coniferyl alcohol
pH 7.5, 30°C, recombinant enzyme mutant MOMT9
0.1926
coniferyl alcohol
pH 7.5, 30°C, recombinant enzyme mutant MOMT4
0.1985
coniferyl alcohol
pH 7.5, 30°C, recombinant enzyme mutant MOMT3
0.003
eugenol
-
0.007
eugenol
plant-purified enzyme
0.03
eugenol
recombinant enzyme
0.0193
isoeugenol
-
pH 7.5, 25°C
0.058
isoeugenol
plant-purified enzyme
0.074
isoeugenol
recombinant enzyme
0.005
S-adenosyl-L-methionine
-
0.019
S-adenosyl-L-methionine
recombinant enzyme
0.027
S-adenosyl-L-methionine
plant-purified enzyme
0.0545
S-adenosyl-L-methionine
-
pH 7.5, 25°C
0.0147
sinapyl alcohol
pH 7.5, 30°C, recombinant enzyme mutant MOMT5
0.0263
sinapyl alcohol
pH 7.5, 30°C, recombinant enzyme mutant MOMT8
0.0681
sinapyl alcohol
pH 7.5, 30°C, recombinant enzyme mutant MOMT4
0.1095
sinapyl alcohol
pH 7.5, 30°C, recombinant enzyme mutant MOMT9
0.1187
sinapyl alcohol
pH 7.5, 30°C, recombinant enzyme mutant MOMT3
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0.049 - 0.528
coniferyl alcohol
0.006 - 0.271
sinapyl alcohol
0.049
coniferyl alcohol
pH 7.5, 30°C, recombinant enzyme mutant MOMT8
0.063
coniferyl alcohol
pH 7.5, 30°C, recombinant enzyme mutant MOMT9
0.069
coniferyl alcohol
pH 7.5, 30°C, recombinant enzyme mutant MOMT5
0.247
coniferyl alcohol
pH 7.5, 30°C, recombinant enzyme mutant MOMT3
0.528
coniferyl alcohol
pH 7.5, 30°C, recombinant enzyme mutant MOMT4
0.0031
eugenol
-
0.015
isoeugenol
-
pH 7.5, 25°C
0.006
sinapyl alcohol
pH 7.5, 30°C, recombinant enzyme mutant MOMT8
0.017
sinapyl alcohol
pH 7.5, 30°C, recombinant enzyme mutant MOMT9
0.033
sinapyl alcohol
pH 7.5, 30°C, recombinant enzyme mutant MOMT5
0.173
sinapyl alcohol
pH 7.5, 30°C, recombinant enzyme mutant MOMT3
0.271
sinapyl alcohol
pH 7.5, 30°C, recombinant enzyme mutant MOMT4
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1.24 - 6.05
coniferyl alcohol
0.78
isoeugenol
-
pH 7.5, 25°C
0.16 - 3.98
sinapyl alcohol
1.24
coniferyl alcohol
pH 7.5, 30°C, recombinant enzyme mutant MOMT3
2.74
coniferyl alcohol
pH 7.5, 30°C, recombinant enzyme mutant MOMT4
3.04
coniferyl alcohol
pH 7.5, 30°C, recombinant enzyme mutant MOMT8
3.91
coniferyl alcohol
pH 7.5, 30°C, recombinant enzyme mutant MOMT9
6.05
coniferyl alcohol
pH 7.5, 30°C, recombinant enzyme mutant MOMT5
0.16
sinapyl alcohol
pH 7.5, 30°C, recombinant enzyme mutant MOMT9
0.23
sinapyl alcohol
pH 7.5, 30°C, recombinant enzyme mutant MOMT8
1.46
sinapyl alcohol
pH 7.5, 30°C, recombinant enzyme mutant MOMT3
2.25
sinapyl alcohol
pH 7.5, 30°C, recombinant enzyme mutant MOMT5
3.98
sinapyl alcohol
pH 7.5, 30°C, recombinant enzyme mutant MOMT4
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0.0017
enzyme variant MOMT4, pH 7.5, 30°C, substrate caffeyl alcohol
0.0025
enzyme variant MOMT8, pH 7.5, 30°C, substrate coumaryl alcohol
0.0037
enzyme variant MOMT9, pH 7.5, 30°C, substrate coumaryl alcohol
0.0055
enzyme variant MOMT4, pH 7.5, 30°C, substrate coumaryl alcohol
0.0065
enzyme variant MOMT5, pH 7.5, 30°C, substrate caffeyl alcohol
0.0077
enzyme variant MOMT8, pH 7.5, 30°C, substrate sinapyl alcohol
0.0096
enzyme variant MOMT5, pH 7.5, 30°C, substrate coumaryl alcohol
0.0098
enzyme variant MOMT9, pH 7.5, 30°C, substrate sinapyl alcohol
0.0108
enzyme variant MOMT8, pH 7.5, 30°C, substrate caffeyl alcohol
0.0129
enzyme variant MOMT9, pH 7.5, 30°C, substrate caffeyl alcohol
0.0396
enzyme variant MOMT5, pH 7.5, 30°C, substrate sinapyl alcohol
0.0514
enzyme variant MOMT8, pH 7.5, 30°C, substrate coniferyl alcohol
0.0668
enzyme variant MOMT4, pH 7.5, 30°C, substrate coniferyl alcohol
0.0683
enzyme variant MOMT9, pH 7.5, 30°C, substrate coniferyl alcohol
0.0796
enzyme variant MOMT4, pH 7.5, 30°C, substrate sinapyl alcohol
0.0806
enzyme variant MOMT5, pH 7.5, 30°C, substrate coniferyl alcohol
0.1929
enzyme variant MOMT3, pH 7.5, 30°C, substrate sinapyl alcohol
0.3463
enzyme variant MOMT3, pH 7.5, 30°C, substrate coniferyl alcohol
additional information
-
additional information
-
-
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CVMT1_OCIBA
356
0
39916
Swiss-Prot
other Location (Reliability: 3)
EOMT1_OCIBA
357
0
40237
Swiss-Prot
other Location (Reliability: 4)
IEMT_CLABR
368
0
39993
Swiss-Prot
other Location (Reliability: 2)
OMT1_SORBI
376
0
40847
Swiss-Prot
other Location (Reliability: 2)
B9RW39_RICCO
351
0
38956
TrEMBL
other Location (Reliability: 3)
B9SGP0_RICCO
356
0
39868
TrEMBL
other Location (Reliability: 3)
B9RVR2_RICCO
175
0
19973
TrEMBL
other Location (Reliability: 4)
B9SGP3_RICCO
127
0
13881
TrEMBL
other Location (Reliability: 5)
A0A4D6DKF2_9LAMI
335
0
37639
TrEMBL
Secretory Pathway (Reliability: 5)
B9RGB0_RICCO
360
0
39427
TrEMBL
other Location (Reliability: 2)
A0A4D6DKB1_9LAMI
336
0
37562
TrEMBL
Secretory Pathway (Reliability: 5)
A0A4D6DKK9_9LAMI
335
0
37274
TrEMBL
Secretory Pathway (Reliability: 5)
A0A4D6DKA5_9LAMI
335
0
37355
TrEMBL
Secretory Pathway (Reliability: 5)
A0A4D6DKA6_9LAMI
327
0
36673
TrEMBL
Secretory Pathway (Reliability: 5)
A0A4D6DKJ4_9LAMI
336
0
37444
TrEMBL
Secretory Pathway (Reliability: 5)
B9T9S0_RICCO
110
0
12725
TrEMBL
Secretory Pathway (Reliability: 5)
B9RVR5_RICCO
362
0
40257
TrEMBL
other Location (Reliability: 2)
B9S932_RICCO
359
0
40513
TrEMBL
other Location (Reliability: 3)
B9TKY3_RICCO
228
0
25633
TrEMBL
Secretory Pathway (Reliability: 2)
B9SGP1_RICCO
356
0
39539
TrEMBL
other Location (Reliability: 3)
B9RVR4_RICCO
372
0
41854
TrEMBL
other Location (Reliability: 2)
B9SGP4_RICCO
321
0
35518
TrEMBL
other Location (Reliability: 1)
A0A4D6DKU0_9LAMI
335
0
37274
TrEMBL
Secretory Pathway (Reliability: 5)
A0A4D6DKF9_9LAMI
334
0
37766
TrEMBL
Secretory Pathway (Reliability: 5)
B9SGP2_RICCO
132
0
14574
TrEMBL
other Location (Reliability: 5)
A0A4D6DKK0_9LAMI
331
0
36999
TrEMBL
Secretory Pathway (Reliability: 5)
A0A4D6DKD2_OCIBA
335
0
37551
TrEMBL
Secretory Pathway (Reliability: 5)
B9S931_RICCO
359
0
40392
TrEMBL
other Location (Reliability: 3)
A0A4D6DK91_9LAMI
332
0
37191
TrEMBL
Secretory Pathway (Reliability: 5)
A0A4D6DK86_9LAMI
335
0
37483
TrEMBL
Secretory Pathway (Reliability: 5)
A0A4D6DKH4_9LAMI
327
0
36705
TrEMBL
Secretory Pathway (Reliability: 5)
A0A4D6DKE5_9LAMI
333
0
37132
TrEMBL
Secretory Pathway (Reliability: 5)
B9RW38_RICCO
352
0
39194
TrEMBL
other Location (Reliability: 4)
B9RVQ9_RICCO
105
0
11912
TrEMBL
other Location (Reliability: 2)
A0A4D6DKH0_9LAMI
333
0
37342
TrEMBL
Secretory Pathway (Reliability: 5)
A0A396JW13_MEDTR
101
0
11465
TrEMBL
other Location (Reliability: 3)
A0A2P6QQ71_ROSCH
366
0
40157
TrEMBL
other Location (Reliability: 2)
A0A4D6DKB6_9LAMI
336
0
37673
TrEMBL
Secretory Pathway (Reliability: 5)
B9RVQ8_RICCO
356
0
39607
TrEMBL
other Location (Reliability: 5)
A0A4D6DKD9_9LAMI
315
0
34928
TrEMBL
other Location (Reliability: 5)
A0A396GKB8_MEDTR
144
0
15899
TrEMBL
other Location (Reliability: 5)
A0A4D6DKG7_9LAMI
336
0
37510
TrEMBL
Secretory Pathway (Reliability: 5)
A0A4D6DKB7_9LAMI
335
0
37288
TrEMBL
Secretory Pathway (Reliability: 5)
A0A4D6DKA0_9LAMI
335
0
37191
TrEMBL
Secretory Pathway (Reliability: 5)
B9RVR3_RICCO
362
0
40599
TrEMBL
other Location (Reliability: 2)
A0A2P6SIU6_ROSCH
362
0
39583
TrEMBL
other Location (Reliability: 1)
A0A4D6DKT6_OCIBA
335
0
37535
TrEMBL
Secretory Pathway (Reliability: 4)
B9SUX6_RICCO
345
0
38300
TrEMBL
other Location (Reliability: 2)
A0A4D6DKJ1_9LAMI
327
0
36549
TrEMBL
Secretory Pathway (Reliability: 5)
A0A4D6DKG3_9LAMI
335
0
37426
TrEMBL
Secretory Pathway (Reliability: 5)
B9RVR6_RICCO
356
0
39851
TrEMBL
other Location (Reliability: 1)
A0A4D6DKU7_9LAMI
335
0
37260
TrEMBL
Secretory Pathway (Reliability: 5)
A0A4D6DKC7_9LAMI
327
0
36608
TrEMBL
Secretory Pathway (Reliability: 5)
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Wang, J.; Pichersky, E.
Characterization of S-adenosyl-L-methionine:(iso)eugenol O-methyltransferase involved in floral scent production in Clarkia breweri
Arch. Biochem. Biophys.
349
153-160
1998
Clarkia breweri (O04385), Clarkia breweri
brenda
Wang, J.; Dudareva, N.; Bhakta, S.; Raguso, R.A.; Pichersky, E.
Floral scent production in Clarkia breweri (Onagraceae). II. Localization and development modulation of the enzyme S-adenosyl-L-methionine:(iso)eugenol O-methyltransferase and phenylpropanoid emission
Plant Physiol.
114
213-221
1997
Clarkia breweri (O04385), Clarkia breweri
brenda
Gang, D.R.; Lavid, N.; Zubieta, C.; Chen, F.; Beuerle, T.; Lewinsohn, E.; Noel, J.P.; Pichersky, E.
Characterization of phenylpropene O-methyltransferases from sweet basil: facile change of substrate specificity and convergent evolution within a plant O-methyltransferase family
Plant Cell
14
505-519
2002
Ocimum basilicum (Q93WU2), Ocimum basilicum
brenda
Wu, S.; Watanabe, N.; Mita, S.; Ueda, Y.; Shibuya, M.; Ebizuka, Y.
Two O-methyltransferases isolated from flower petals of Rosa chinensis var. spontanea involved in scent biosynthesis
J. Biosci. Bioeng.
96
119-128
2003
Rosa chinensis
brenda
Barkman, T.J.
Evidence for positive selection on the floral scent gene isoeugenol-O-methyltransferase
Mol. Biol. Evol.
20
168-172
2003
Clarkia breweri
brenda
Koeduka, T.; Baiga, T.J.; Noel, J.P.; Pichersky, E.
Biosynthesis of t-anethole in anise: characterization of t-anol/isoeugenol synthase and an O-methyltransferase specific for a C7-C8 propenyl side chain
Plant Physiol.
149
384-394
2009
Pimpinella anisum
brenda
Tarchoune, I.; Batour, O.; Harrathi, J.; Hamdaoui, G.; Lachal, M.; Ouerghi, Z.; Marzouk, B.
Effects of two sodium salts on fatty acid and essential oil composition of basil (Ocimum basilicum L.) leaves
Acta Physiol. Plant.
35
2365-2372
2013
Ocimum basilicum
-
brenda
Renu, I.K.; Haque, I.; Kumar, M.; Poddar, R.; Bandopadhyay, R.; Rai, A.; Mukhopadhyay, K.
Characterization and functional analysis of eugenol O-methyltransferase gene reveal metabolite shifts, chemotype specific differential expression and developmental regulation in Ocimum tenuiflorum L
Mol. Biol. Rep.
41
1857-1870
2014
Ocimum tenuiflorum (Q93WU2), Ocimum tenuiflorum
brenda
Cai, Y.; Bhuiya, M.; Shanklin, J.; Liu, C.
Engineering a monolignol 4-O-methyltransferase with high selectivity for the condensed lignin precursor coniferyl alcohol
J. Biol. Chem.
290
26715-26724
2015
Clarkia breweri (O04385)
brenda