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(S)-tetrahydroprotoberberine cis-N-methyltransferase
(S)-Tetrahydroprotoberberine-cis-N-methyltransferase
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Methyltransferase, tetrahydroberberine N-
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S-Adenosyl-L-methionine: (S)-7,8,13,14-tetrahydroberberine-cis-N-methyltransferase
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S-Adenosyl-L-methionine:tetrahydroberberine-cis-N-methyltransferase
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S-adenosyl-L-methionine:tetrahydroprotoberberine cis-N-methyltransferase
S-Adenosylmethionine:tetrahydroberberine-N-methyltransferase
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Tetrahydroberberine cis-N-methyltransferase
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(S)-tetrahydroprotoberberine cis-N-methyltransferase
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(S)-tetrahydroprotoberberine cis-N-methyltransferase
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(S)-tetrahydroprotoberberine cis-N-methyltransferase
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(S)-tetrahydroprotoberberine cis-N-methyltransferase
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(S)-tetrahydroprotoberberine cis-N-methyltransferase
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(S)-tetrahydroprotoberberine cis-N-methyltransferase
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(S)-tetrahydroprotoberberine cis-N-methyltransferase
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S-adenosyl-L-methionine:tetrahydroprotoberberine cis-N-methyltransferase
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S-adenosyl-L-methionine:tetrahydroprotoberberine cis-N-methyltransferase
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S-adenosyl-L-methionine:tetrahydroprotoberberine cis-N-methyltransferase
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S-adenosyl-L-methionine:tetrahydroprotoberberine cis-N-methyltransferase
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S-adenosyl-L-methionine:tetrahydroprotoberberine cis-N-methyltransferase
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S-adenosyl-L-methionine:tetrahydroprotoberberine cis-N-methyltransferase
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S-adenosyl-L-methionine:tetrahydroprotoberberine cis-N-methyltransferase
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TNMT
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S-adenosyl-L-methionine + (R,S)-canadine
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?
S-adenosyl-L-methionine + (R,S)-stylopine
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S-adenosyl-L-methionine + (R,S)-tetrahydropalmatine
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S-Adenosyl-L-methionine + (S)-7,8,13,14-tetrahydroprotoberberine
S-Adenosyl-L-homocysteine + cis-N-methyl-(S)-7,8,13,14-tetrahydroprotoberberine
S-adenosyl-L-methionine + (S)-canadine
?
S-Adenosyl-L-methionine + (S)-stylopine
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S-Adenosyl-L-methionine + tetrahydrojatrorrhizine
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?
S-Adenosyl-L-methionine + tetrahydropalmatrubine
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?
additional information
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S-adenosyl-L-methionine + (R,S)-stylopine
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?
S-adenosyl-L-methionine + (R,S)-stylopine
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?
S-adenosyl-L-methionine + (R,S)-stylopine
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?
S-adenosyl-L-methionine + (R,S)-stylopine
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S-adenosyl-L-methionine + (R,S)-stylopine
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S-adenosyl-L-methionine + (R,S)-stylopine
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strict substrate specificity, protoberberine alkaloids tetrahydroxyberbine and scoulerine, simple isoquinoline, benzylisoquinoline, and pavine alkaloids not accepted as substrates
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?
S-adenosyl-L-methionine + (R,S)-stylopine
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?
S-adenosyl-L-methionine + (R,S)-tetrahydropalmatine
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?
S-adenosyl-L-methionine + (R,S)-tetrahydropalmatine
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used for product identification
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S-Adenosyl-L-methionine + (S)-7,8,13,14-tetrahydroprotoberberine
S-Adenosyl-L-homocysteine + cis-N-methyl-(S)-7,8,13,14-tetrahydroprotoberberine
Berberis stenophylla
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?
S-Adenosyl-L-methionine + (S)-7,8,13,14-tetrahydroprotoberberine
S-Adenosyl-L-homocysteine + cis-N-methyl-(S)-7,8,13,14-tetrahydroprotoberberine
Corydalis vaginans
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?
S-Adenosyl-L-methionine + (S)-7,8,13,14-tetrahydroprotoberberine
S-Adenosyl-L-homocysteine + cis-N-methyl-(S)-7,8,13,14-tetrahydroprotoberberine
Corydalis vaginans
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?
S-Adenosyl-L-methionine + (S)-7,8,13,14-tetrahydroprotoberberine
S-Adenosyl-L-homocysteine + cis-N-methyl-(S)-7,8,13,14-tetrahydroprotoberberine
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?
S-Adenosyl-L-methionine + (S)-7,8,13,14-tetrahydroprotoberberine
S-Adenosyl-L-homocysteine + cis-N-methyl-(S)-7,8,13,14-tetrahydroprotoberberine
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?
S-Adenosyl-L-methionine + (S)-7,8,13,14-tetrahydroprotoberberine
S-Adenosyl-L-homocysteine + cis-N-methyl-(S)-7,8,13,14-tetrahydroprotoberberine
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?
S-Adenosyl-L-methionine + (S)-7,8,13,14-tetrahydroprotoberberine
S-Adenosyl-L-homocysteine + cis-N-methyl-(S)-7,8,13,14-tetrahydroprotoberberine
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?
S-Adenosyl-L-methionine + (S)-7,8,13,14-tetrahydroprotoberberine
S-Adenosyl-L-homocysteine + cis-N-methyl-(S)-7,8,13,14-tetrahydroprotoberberine
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?
S-adenosyl-L-methionine + (S)-canadine
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Corydalis vaginans
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S-adenosyl-L-methionine + (S)-canadine
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S-Adenosyl-L-methionine + (S)-stylopine
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Berberis stenophylla
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S-Adenosyl-L-methionine + (S)-stylopine
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Corydalis vaginans
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S-Adenosyl-L-methionine + (S)-stylopine
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Corydalis vaginans
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at 77% of the activity with (S)-canadine
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S-Adenosyl-L-methionine + (S)-stylopine
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S-Adenosyl-L-methionine + (S)-stylopine
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S-Adenosyl-L-methionine + (S)-stylopine
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S-Adenosyl-L-methionine + (S)-stylopine
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S-Adenosyl-L-methionine + (S)-stylopine
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additional information
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dimethoxytetrahydroisoquinoline and methylisoquinolinediol, norlaudanosoline, (R,S)-tetrahydroxyberbine and (S)-scoulerine, or (R,S)-pavine are not accepted as substrates
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additional information
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dimethoxytetrahydroisoquinoline and methylisoquinolinediol, norlaudanosoline, (R,S)-tetrahydroxyberbine and (S)-scoulerine, or (R,S)-pavine are not accepted as substrates
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Mg2+
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slight increase in activity with increasing concentrations of Mg2+ in sodium phosphate buffer, inhibitory in Hepes buffer
Mn2+
inhibits activity by 41%, inhibition prevented by inclusion of 10 mM EDTA; inhibits TNMT activity by 41%, can be prevented by the inclusion of EDTA
Ni2+
inhibits activity by 72%, inhibition prevented by inclusion of 10 mM EDTA; inhibits TNMT activity by 72%, can be prevented by the inclusion of EDTA
S-adenosyl-L-homocysteine
Zn2+
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in sodium phosphate buffer and in Hepes buffer
Ca2+
inhibits activity by 62%, inhibition prevented by inclusion of 10 mM EDTA; inhibits TNMT activity by 62%, can be prevented by the inclusion of EDTA
Ca2+
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in Hepes buffer, not inhibitory in sodium phosphate buffer
Co2+
inhibits activity by 71%, inhibition prevented by inclusion of 10 mM EDTA; inhibits TNMT activity by 71%, can be prevented by the inclusion of EDTA
Co2+
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in sodium phosphate buffer and in Hepes buffer
S-adenosyl-L-homocysteine
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S-adenosyl-L-homocysteine
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additional information
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not inhibited by MgCl2
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additional information
not inhibited by MgCl2
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O'Keefe, B.R.; Beecher, C.W.W.
Isolation and characterization of S-adenosyl-L-methionine:tetrahydroberberibe-cis-N-methyltransferase from suspension cultures of Sanguinaria canadensis L.
Plant Physiol.
105
395-403
1994
Sanguinaria canadensis
brenda
Rueffer, M.; Zumstein, G.; Zenk, M.H.
Partial purification and properties of S-adenosyl-L-methionine:(S)-tetrahydroprotoberberine-cis-N-methyltransferase from suspension-cultured cells of Eschscholtzia and Corydalis
Phytochemistry
29
3727-3733
1990
Corydalis vaginans, Eschscholzia californica
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brenda
Rueffer, M.; Zenk, M.H.
S-Adenosyl-L-methionine: (S)-7,8,13,14-tetrahydroberberine-cis-N-methyltransferase, a branch point enzyme in the biosynthesis of benzophenanthridine and protopine alkaloids
Tetrahedron Lett.
27
5603-5604
1986
Berberis stenophylla, Corydalis vaginans, Lamprocapnos spectabilis, Fumaria officinalis, Papaver somniferum
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brenda
Liscombe, D.K.; Facchini, P.J.
Molecular cloning and characterization of tetrahydroprotoberberine cis-N-methyltransferase, an enzyme involved in alkaloid biosynthesis in opium poppy
J. Biol. Chem.
282
14741-14751
2007
Chelidonium majus, Eschscholzia californica, no activity in Nicotiana tabacum, Papaver bracteatum, Papaver somniferum, Papaver somniferum (Q108P1), Sanguinaria canadensis, Thalictrum flavum, Argemone mexicana, no activity in Nandina domestica, no activity in Tinospora cordifolia
brenda