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1-cyclohexyl-3-(2-morpholinoethyl)-carbodiimide metho-p-toluenesulfonate
-
preincubation with NO2- completely protects
2-heptyl-4-hydroxyquinoline N-oxide
-
-
antimycin A
-
0.2 mM, 45% inhibition
Hydrazine sulfate
-
complete inhibition
iodoacetate
-
64% inhibition
KCNS
-
0.1 mM, 90% inhibition of NH2OH reductase activity, nitrite reductase activity is not affected
NADP+
-
0.06 mM, 13% inhibition, 0.27 mM, 43% inhibition
NH2OH
-
competitive vs. NO2-
NH4+
-
inhibits under aerobic and anaerobic conditions
O2
-
enzyme activity under anaerobic conditions is 2fold higher than under aerobic conditions
Rose bengal
-
preincubation with NO2- completely protects
rotenone
-
0.1 mM, 56% inhibition
Sodium diethyldithiocarbamate
-
-
1,10-phenanthroline
-
-
2,2'-bipyridine
-
1 mM, 40% inhibition, no inhibition if enzyme is preincubated with nitrite or NH2OH prior to adding the inhibitor
8-hydroxyquinoline
-
1 mM, 96% inhibition
arsenite
-
10 mM, 24% inhibition
arsenite
-
0.5 mM, 62% inhibition
CN-
-
0.01 mM, 40% inhibition, 0.1 mM, 93% inhibition
CN-
-
1 mM, 45% inhibition, no inhibition if enzyme is preincubated with nitrite or NH2OH prior to adding the inhibitor
CN-
-
0.02 mM, 88% inhibition
CN-
-
1 mM, complete inhibition of activity in cytosolic extracts
CO
-
competitive vs. nitrite
CO
-
almost complete inhibition
hydroxylamine
-
competitive vs. nitrite
N-ethylmaleimide
-
1 mM, 64% inhibition after 5 min, reduced glutathione restores activity to 94%
N-ethylmaleimide
-
0.1 mM, 52% inhibition
NAD(P)H
-
inactivation after preincubation, presence of nitrite protects from inactivation
NAD(P)H
-
in vitro inactivation after preincubation for 5-10 min in the presence of FAD
NAD+
-
hydroxylamine reduction, substrate inhibition at low concentration, mixed inhibition vs. NADH
NAD+
-
substrate inhibition at high concentration, required for full activity at low concentrations, maximum at 1 mM
NaN3
-
-
nitrite
-
competitive vs. hydroxylamine
nitrite
-
substrate inhibition with increasing concentrations, complete inhibition with 50 mM nitrite
p-chloromercuribenzoate
-
1 mM, 79% inhibition after 5 min, dithiothreitol completely reverses activity, reduced glutathione, L-cysteine and 2-mercaptoethanol reverse activity to 86%, 64% and 82% respectively
p-chloromercuribenzoate
-
0.1 mM, 60% inhibition
p-chloromercuribenzoate
-
0.02 mM, complete inactivation, partially prevented by 10 mM cysteine
p-chloromercuribenzoate
-
0.001 mM, 83% inhibition
p-chloromercuribenzoate
-
-
p-chloromercuribenzoate
-
completely reversed by glutathione
p-hydroxymercuribenzoate
-
0.01 mM, 42% inhibition, 0.1 mM, complete inhibition
p-hydroxymercuribenzoate
-
very potent inhibitor, inhibition can be reversed by either cysteine or glutathione
SO32-
-
1 mM, 22% inhibition
additional information
-
intracellularly accumulated nitrite can inhibit nitrate uptake, addition of nitrite at concentrations above 5 mM is toxic and causes growth retardation
-
additional information
-
a progressive inhibition of activity is observed with increasing level of salinity
-
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-
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