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6-methoxy-3-methyl-2-(all-trans-polyprenyl)-1,4-benzoquinol + a reduced acceptor + O2
3-demethylubiquinol + acceptor + H2O
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6-methoxy-3-methyl-2-all-trans-octaprenyl-1,4-benzoquinol + a reduced electron acceptor + oxygen
3-demethylubiquinol-8 + H2O + an oxidized electron acceptor
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-
-
?
6-methoxy-3-methyl-2-all-trans-octaprenyl-1,4-benzoquinol + a reduced electron acceptor + oxygen
an oxidized electron acceptor + 3-demethylubiquinol-8 + H2O
6-methoxy-3-methyl-2-all-trans-octaprenyl-1,4-benzoquinol + a reduced electron acceptor + oxygen
an oxidized electron acceptor + 3-demethylubiquinol-8 + H2O
the enzyme catalyzes a reaction in the ubiquinone biosynthesis pathway
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-
?
6-methoxy-3-methyl-2-all-trans-octaprenyl-1,4-benzoquinol + a reduced electron acceptor + oxygen
an oxidized electron acceptor + 3-demethylubiquinol-8 + H2O
the ubiF mutant accumulates 2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinone. The enzyme has not been characterized biochemically
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malfunction
the ubiF mutant shows poor growth on glucose and no growth on succinate, the ubiF mutant accumulates 2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinone
physiological function
the enzyme catalyzes a reaction in the ubiquinone biosynthesis pathway
physiological function
a plasmid encoding CLK-1 supports aerobic respiration on a nonfermentable carbon source of the Escherichia coli ubiF mutant strain and rescues the ability to synthesize ubiquinone
physiological function
COQ7-deficient mouse embryos fail to survive beyond embryonic day 10.5, exhibit small-sized body and delayed embryogenesis. COQ7-deficient neuroepithelial cells fail to show the radial arrangement in the developing cerebral wall, aborting neurogenesis at E10.5. The enlarged mitochondria with vesicular cristae and enlarged lysosomes fill with disrupted membranes, which is consistent with mitochondriopathy. COQ7-deficient embryos fail to synthesize CoQ9, but instead yield demethoxyubiquinone 9
physiological function
deletion of the chromosomal Coq7 gene generates respiration defective yeast mutants deficient in ubiquinone. Coq mutants accumulate an early intermediate in the ubiquinone biosynthetic pathway, 3-hexaprenyl-4-hydroxybenzoate
physiological function
enzyme is directly involved in ubiquinone biosynthesis. The defect in gluconeogenic gene activation in Coq7/Cat5 null mutants is a general consequence of a defect in respiration
physiological function
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mutation lacking UbiF activity accumulate 2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinone
physiological function
promoter-driven COQ7/CLK-1 expression providing low levels of transgene expression rescues COQ7-deficient mice from embryonic lethality. The mice show a concomitant decrease in CoQ9, mitochondrial respiratory enzyme activity and the generation of reactive oxygen species in the mitochondria
physiological function
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the Escherichia coli UbiF gene expressed at low copy restores growth of Coq7 point mutant E194K on medium containing a non-fermentable carbon source, but fails to rescue a coq7 null mutant. Expression of UbiF from a multicopy vector restores growth and coenzyme Q synthesis for both mutants, although with a higher efficiency in the point mutant. Addition of coenzyme Q to the growth media also stabilizes the Coq3 and Coq4 polypeptides in the coq7 null mutant
physiological function
the gene complements an Escherichia coli mutant that lacks 5-demethoxyubiquinone hydroxylase UbiF
physiological function
the human CLK-1 gene is able to functionally complement Saccharomyces cerevisiae Coq7 deletion mutants
physiological function
transgenic expression of mouse CoQ7 in Caenorhabditis elegans completely rescues the slowed rhythmic behaviors of Clk-1 mutants such as defecation. In life-span analysis, transgenic expression reverts the extended life span of Clk-1 to a comparable level with wild-type control
physiological function
Caenorhabditis elegans Bristol 2
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a plasmid encoding CLK-1 supports aerobic respiration on a nonfermentable carbon source of the Escherichia coli ubiF mutant strain and rescues the ability to synthesize ubiquinone
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Kwon, O.; Kotsakis, A.; Meganathan, R.
Ubiquinone (coenzyme Q) biosynthesis in Escherichia coli: identification of the ubiF gene
FEMS Microbiol. Lett.
186
157-161
2000
Escherichia coli (P75728)
brenda
Nakai, D.; Shimizu, T.; Nojiri, H.; Uchiyama, S.; Koike, H.; Takahashi, M.; Hirokawa, K.; Shirasawa, T.
coq7/clk-1 regulates mitochondrial respiration and the generation of reactive oxygen species via coenzyme Q
Aging Cell
3
273-281
2004
Mus musculus (P97478), Mus musculus
brenda
Takahashi, M.; Asaumi, S.; Honda, S.; Suzuki, Y.; Nakai, D.; Kuroyanagi, H.; Shimizu, T.; Honda, Y.; Shirasawa, T.
Mouse coq7/clk-1 orthologue rescued slowed rhythmic behavior and extended life span of clk-1 longevity mutant in Caenorhabditis elegans
Biochem. Biophys. Res. Commun.
286
534-540
2001
Mus musculus (P97478), Mus musculus
brenda
Nakai, D.; Yuasa, S.; Takahashi, M.; Shimizu, T.; Asaumi, S.; Isono, K.; Takao, T.; Suzuki, Y.; Kuroyanagi, H.; Hirokawa, K.; Koseki, H.; Shirsawa, T.
Mouse homologue of coq7/clk-1, longevity gene in Caenorhabditis elegans, is essential for coenzyme Q synthesis, maintenance of mitochondrial integrity, and neurogenesis
Biochem. Biophys. Res. Commun.
289
463-471
2001
Mus musculus (P97478), Mus musculus
brenda
Rea, S.
CLK-1/Coq7p is a DMQ mono-oxygenase and a new member of the di-iron carboxylate protein family
FEBS Lett.
509
389-394
2001
Rattus norvegicus (Q63619)
brenda
Adachi, A.; Shinjyo, N.; Fujita, D.; Miyoshi, H.; Amino, H.; Watanabe, Y.i.; Kita, K.
Complementation of Escherichia coli ubiF mutation by Caenorhabditis elegans CLK-1, a product of the longevity gene of the nematode worm
FEBS Lett.
543
174-178
2003
Caenorhabditis elegans (P48376), Caenorhabditis elegans, Caenorhabditis elegans Bristol 2 (P48376)
brenda
Kwon, O.; Kotsakis, A.; Meganathan, R.
Ubiquinone (coenzyme Q) biosynthesis in Escherichia coli Identification of the ubiF gene
FEMS Microbiol. Lett.
186
157-161
2000
Escherichia coli (P75728)
brenda
Young, I.; McCann, L.; Stroobant, P.; Gibson, F.
Characterization and genetic analysis of mutant strains of Escherichia coli K-12 accumulating the biquinone precursors 2-octaprenyl-6-methoxy-1,4-benzoquinone and 2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinone
J. Bacteriol.
105
769-778
1971
Escherichia coli
brenda
Marbois, B.; Clarke, C.
The COQ7 gene encodes a protein in Saccharomyces cerevisiae necessary for ubiquinone biosynthesis
J. Biol. Chem.
271
2995-3004
1996
Saccharomyces cerevisiae (P41735)
brenda
Stenmark, P.; Gruenler, J.; Mattsson, J.; Sindelar, P.J.; Nordlund, P.; Berthold, D.A.
A new member of the family of di-iron carboxylate proteins. Coq7 (clk-1), a membrane-bound hydroxylase involved in ubiquinone biosynthesis
J. Biol. Chem.
276
33297-33300
2001
Pseudomonas aeruginosa (Q9I5R6), Pseudomonas aeruginosa
brenda
Jonassen, T.; Davis, D.E.; Larsen, P.L.; Clarke, C.F.
Reproductive fitness and quinone content of Caenorhabditis elegans clk-1 mutants fed coenzyme Q isoforms of varying length
J. Biol. Chem.
278
51735-51742
2003
Saccharomyces cerevisiae (P41735)
brenda
Tran, U.; Marbois, B.; Gin, P.; Gulmezian, M.; Jonassen, T.; Clarke, C.
Complementation of Saccharomyces cerevisiae coq7 mutants by mitochondrial targeting of the Escherichia coli UbiF polypeptide Two functions of yeast Coq7 polypeptide in coenzyme Q biosynthesis
J. Biol. Chem.
281
16401-16409
2006
Saccharomyces cerevisiae
brenda
Vajo, Z.; King, L.M.; Jonassen, T.; Wilkin, D.J.; Ho, N.; Munnich, A.; Clarke, C.F.; Francomano, C.A.
Conservation of the Caenorhabditis elegans timing gene clk-1 from yeast to human a gene required for ubiquinone biosynthesis with potential implications for aging
Mamm. Genome
10
1000-1004
1999
Homo sapiens (Q99807), Homo sapiens
brenda