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Information on EC 1.14.14.69 - ent-cassadiene hydroxylase

for references in articles please use BRENDA:EC1.14.14.69
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IUBMB Comments
A cytochrome P-450 (heme-thiolate) protein isolated from the plant Oryza sativa (rice) that is involved in phytocassanes biosynthesis. Depending on the order of activities, the enzyme may form either ent-cassa-12,15-dien-2-one or ent-cassa-12,15-diene-2beta,3beta-diol as an intermediate.
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The expected taxonomic range for this enzyme is: Oryza sativa Japonica Group
Synonyms
cyp71z7, more
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ent-cassa-12,15-dien-2-one + [reduced NADPH-hemoprotein reductase] + O2 = ent-3beta-hydroxycassa-12,15-dien-2-one + [oxidized NADPH-hemoprotein reductase] + H2O
show the reaction diagram
(1c)
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ent-cassa-12,15-dien-2beta-ol + [reduced NADPH-hemoprotein reductase] + O2 = ent-cassa-12,15-dien-2-one + [oxidized NADPH-hemoprotein reductase] + 2 H2O
show the reaction diagram
(1b)
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ent-cassa-12,15-dien-2beta-ol + [reduced NADPH-hemoprotein reductase] + O2 = ent-cassa-12,15-diene-2beta,3beta-diol + [oxidized NADPH-hemoprotein reductase] + H2O
show the reaction diagram
(1b')
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ent-cassa-12,15-diene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2 = ent-3beta-hydroxycassa-12,15-dien-2-one + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
show the reaction diagram
overall reaction
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ent-cassa-12,15-diene + [reduced NADPH-hemoprotein reductase] + O2 = ent-cassa-12,15-dien-2beta-ol + [oxidized NADPH-hemoprotein reductase] + H2O
show the reaction diagram
(1a)
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ent-cassa-12,15-diene-2beta,3beta-diol + [reduced NADPH-hemoprotein reductase] + O2 = ent-3beta-hydroxycassa-12,15-dien-2-one + [oxidized NADPH-hemoprotein reductase] + 2 H2O
show the reaction diagram
(1c')
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