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1,2,4-benzenetriol + NADH + O2
?
2,4-dinitrotoluene + NADH + H+ + O2
4-methyl-5-nitrocatechol + nitrite + NAD+
2,4-dinitrotoluene + NADH + O2
4-methyl-5-nitrocatechol + nitrite + NAD+
2,6-dinitrotoluene + NADH + O2
3-methyl-4-nitrocatechol + nitrite + NAD+
2-amino-3-nitrotoluene + NADH + H+ + O2
2-amino-3-nitrobenzylalcohol + NAD+ + H2O
2-amino-3-nitrotoluene + NADH + O2
2-amino-3-nitrobenzylalcohol + nitrite + NAD+
2-amino-5-nitrotoluene + NADH + H+ + O2
2-amino-5-nitrobenzyl alcohol + aminonitrocresol + NAD+ + H2O
2-amino-5-nitrotoluene + NADH + O2
2-amino-5-nitrobenzylalcohol + aminonitrocresol + nitrite + NAD+
2-amino-6-nitrotoluene + NADH + H+ + O2
2-amino-4-nitro-3-cresol + 3-amino-5-nitro-4-cresol + NAD+ + H2O
2-amino-6-nitrotoluene + NADH + O2
2-amino-4-nitro-m-cresol + 3-amino-5-nitro-p-cresol + nitrite + NAD+
2-methoxyphenol + NADH + O2
methoxyhydroquinone + NAD+ + H2O
2-nitrophenol + NADH + O2
nitrohydroquinone + 3-nitrocatechol + NAD+ + H2O
2-nitrotoluene + NADH + H+ + O2
2-nitrobenzyl alcohol + NAD+ + ?
2-nitrotoluene + NADH + H+ + O2
2-nitrobenzyl alcohol + NAD+ + H2O
2-nitrotoluene + NADH + O2
?
3-amino-4-nitrotoluene + NADH + H+ + O2
3-amino-4-nitrobenzyl alcohol + aminonitrocresol + NAD+ + H2O
-
-
-
?
3-amino-4-nitrotoluene + NADH + O2
3-amino-4-nitrobenzylalcohol + aminonitrocresol + nitrite + NAD+
3-methyl-4-nitrophenol + NADH + O2
?
3-nitrophenol + NADH + O2
4-nitrocatechol + NAD+ + H2O
3-nitrotoluene + NADH + H+ + O2
3-nitrobenzyl alcohol + NAD+ + ?
3-nitrotoluene + NADH + O2
4-methylcatechol + nitrite + NAD+
4-amino-2-nitrotoluene + NADH + H+ + O2
aminonitrocresol + NAD+ + H2O
-
-
-
?
4-amino-2-nitrotoluene + NADH + O2
aminonitrocresol + nitrite + NAD+
13% yield
-
-
?
4-amino-3-nitrotoluene + NADH + O2
4-amino-3-nitrobenzylalcohol + aminonitrocresol + ?
-
-
-
?
4-amino-3-nitrotoluene + NADH + O2
4-amino-3-nitrobenzylalcohol + aminonitrocresol + nitrite + NAD+
19% yield
-
-
?
4-methyl-5-nitrocatechol + nitrite + NAD+
2,4-dinitrotoluene + NADH + O2
-
-
-
-
r
4-nitrocatechol + NADH + O2
?
-
low activity
-
-
?
4-nitrotoluene + NADH + H+ + O2
4-nitrobenzyl alcohol + NAD+ + ?
4-nitrotoluene + NADH + O2
4-methylcatechol + nitrite + NAD+
-
-
-
?
5-amino-2-nitrotoluene + NADH + H+ + O2
2-amino-5-nitro-o-cresol + NAD+ + H2O
-
-
-
?
5-amino-2-nitrotoluene + NADH + O2
2-amino-5-nitro-o-cresol + nitrite + NAD+
-
24% yield
-
?
acenaphthene + NADH + H+ + O2
1-acenaphthenol + NAD+ + ?
-
-
-
?
indan + NADH + H+ + O2
1-indanol + 1-indanone + indene + NAD+
-
-
-
?
indan + NADH + H+ + O2
1-indanol + ? + NAD+
indene + NADH + H+ + O2
1-indenol + 1,2-dihydroxyindan + NAD+
-
-
-
?
indole + NADH + H+ + O2
indigo + NAD+ + ?
-
-
-
?
indoline + NADH + H+ + O2
indigo + ? + NAD+
indoline + NADH + H+ + O2
indole + NAD+ + ?
-
-
-
?
m-cresol + NADH + H+ + O2
methylhydroquinone + nitrite + NAD+
m-methoxyphenol + NADH + H+ + O2
methoxyhydroquinone + nitrite + NAD+
m-nitrophenol + NADH + H+ + O2
4-nitrocatechol + nitrite + NAD+
naphthalene + NADH + H+ + O2
(+)-cis-(1R,2S)-dihydroxy-1,2-dihydronaphthalene + NAD+
-
-
-
?
naphthalene + NADH + H+ + O2
(+)-cis-(1R,2S)-dihydroxy-1,2-dihydronaphthalene + NAD+ + ?
naphthalene + NADH + H+ + O2
(1R,2S)-cis-1,2-dihydroxy-1,2-dihydronaphthalene + NAD+
naphthalene + NADH + O2
(1R,3S)-cis-1,2-dihydro-1,2-dihydroxynaphthalene + NAD+ + H2O
-
-
-
-
?
naphthalene + O2
cis-1,2-dihydroxy-1,2-dihydronaphthalene
o-cresol + NADH + H+ + O2
methylhydroquinone + 2-hydroxybenzyl alcohol + nitrite + NAD+
-
mutant V350F produces methylhydroquinone at 1.52 nmol/min/mg of protein and 2-hydroxybenzyl alcohol at 0.74 nmol/min/mg of protein. Mutant V350M produces methylhydroquinone at 1.97 nmol/min/mg of protein and 2-hydroxybenzyl alcohol at 0.11 nmol/min/mg of protein
-
?
o-methoxyphenol + NADH + H+ + O2
methoxyhydroquinone + nitrite + NAD+
-
mutant V350F produces methoxyhydroquinone at 2.5 nmol/min/mg of protein. Mutant V350M produces methoxyhydroquinone at 2.4 mol/min/mg of protein
-
?
o-nitrophenol + NADH + H+ + O2
nitrohydroquinone + 3-nitrocatechol + nitrite + NAD+
-
product of wild-type. Mutant V350F produces both nitrohydroquinone at a rate of 0.75 nmol/min/mg of protein and 3-nitrocatechol at a rate of 0.069 nmol/min/mg of protein from o-nitrophenol. Mutant V350M produces both nitrohydroquinone at 0.33 nmol/min/mg of protein and 3-nitrocatechol at 0.089 nmol/min/mg of protein
-
?
phenetole + NADH + H+ + O2
phenol + NAD+ + ?
-
-
-
?
additional information
?
-
1,2,4-benzenetriol + NADH + O2
?
-
-
-
-
?
1,2,4-benzenetriol + NADH + O2
?
-
-
-
-
?
2,4-dinitrotoluene + NADH + H+ + O2
4-methyl-5-nitrocatechol + nitrite + NAD+
-
-
-
-
?
2,4-dinitrotoluene + NADH + H+ + O2
4-methyl-5-nitrocatechol + nitrite + NAD+
-
-
-
?
2,4-dinitrotoluene + NADH + H+ + O2
4-methyl-5-nitrocatechol + nitrite + NAD+
-
-
-
-
?
2,4-dinitrotoluene + NADH + H+ + O2
4-methyl-5-nitrocatechol + nitrite + NAD+
-
-
-
?
2,4-dinitrotoluene + NADH + H+ + O2
4-methyl-5-nitrocatechol + nitrite + NAD+
-
-
-
-
?
2,4-dinitrotoluene + NADH + H+ + O2
4-methyl-5-nitrocatechol + nitrite + NAD+
-
-
-
?
2,4-dinitrotoluene + NADH + H+ + O2
4-methyl-5-nitrocatechol + nitrite + NAD+
-
-
-
-
?
2,4-dinitrotoluene + NADH + H+ + O2
4-methyl-5-nitrocatechol + nitrite + NAD+
-
-
-
?
2,4-dinitrotoluene + NADH + O2
4-methyl-5-nitrocatechol + nitrite + NAD+
-
highest activity
-
-
?
2,4-dinitrotoluene + NADH + O2
4-methyl-5-nitrocatechol + nitrite + NAD+
-
highest activity
-
-
?
2,4-dinitrotoluene + NADH + O2
4-methyl-5-nitrocatechol + nitrite + NAD+
-
-
-
?
2,4-dinitrotoluene + NADH + O2
4-methyl-5-nitrocatechol + nitrite + NAD+
-
-
-
?
2,4-dinitrotoluene + NADH + O2
4-methyl-5-nitrocatechol + nitrite + NAD+
-
-
-
?
2,4-dinitrotoluene + NADH + O2
4-methyl-5-nitrocatechol + nitrite + NAD+
-
-
formation of 9.54 nmol 3-methylcatechol per mg protein plus 1.06 nmol 2-nitrobenzyl alcohol
-
?
2,4-dinitrotoluene + NADH + O2
4-methyl-5-nitrocatechol + nitrite + NAD+
best substrate
-
-
?
2,4-dinitrotoluene + NADH + O2
4-methyl-5-nitrocatechol + nitrite + NAD+
-
-
-
?
2,4-dinitrotoluene + NADH + O2
4-methyl-5-nitrocatechol + nitrite + NAD+
-
-
-
?
2,4-dinitrotoluene + NADH + O2
4-methyl-5-nitrocatechol + nitrite + NAD+
-
-
formation of 9.54 nmol 3-methylcatechol per mg protein plus 1.06 nmol 2-nitrobenzyl alcohol
-
?
2,4-dinitrotoluene + NADH + O2
4-methyl-5-nitrocatechol + nitrite + NAD+
-
-
-
?
2,4-dinitrotoluene + NADH + O2
4-methyl-5-nitrocatechol + nitrite + NAD+
best substrate
-
-
?
2,4-dinitrotoluene + NADH + O2
4-methyl-5-nitrocatechol + nitrite + NAD+
-
-
-
-
?
2,4-dinitrotoluene + NADH + O2
4-methyl-5-nitrocatechol + nitrite + NAD+
-
-
-
-
?
2,4-dinitrotoluene + NADH + O2
4-methyl-5-nitrocatechol + nitrite + NAD+
-
-
-
-
r
2,4-dinitrotoluene + NADH + O2
4-methyl-5-nitrocatechol + nitrite + NAD+
-
-
-
-
r
2,4-dinitrotoluene + NADH + O2
4-methyl-5-nitrocatechol + nitrite + NAD+
-
-
-
-
?
2,6-dinitrotoluene + NADH + O2
3-methyl-4-nitrocatechol + nitrite + NAD+
-
-
-
?
2,6-dinitrotoluene + NADH + O2
3-methyl-4-nitrocatechol + nitrite + NAD+
-
-
-
?
2-amino-3-nitrotoluene + NADH + H+ + O2
2-amino-3-nitrobenzylalcohol + NAD+ + H2O
-
-
-
?
2-amino-3-nitrotoluene + NADH + H+ + O2
2-amino-3-nitrobenzylalcohol + NAD+ + H2O
-
-
-
?
2-amino-3-nitrotoluene + NADH + O2
2-amino-3-nitrobenzylalcohol + nitrite + NAD+
-
27% yield
-
?
2-amino-3-nitrotoluene + NADH + O2
2-amino-3-nitrobenzylalcohol + nitrite + NAD+
-
27% yield
-
?
2-amino-5-nitrotoluene + NADH + H+ + O2
2-amino-5-nitrobenzyl alcohol + aminonitrocresol + NAD+ + H2O
-
-
-
?
2-amino-5-nitrotoluene + NADH + H+ + O2
2-amino-5-nitrobenzyl alcohol + aminonitrocresol + NAD+ + H2O
-
-
-
?
2-amino-5-nitrotoluene + NADH + O2
2-amino-5-nitrobenzylalcohol + aminonitrocresol + nitrite + NAD+
-
34.4% yield
-
?
2-amino-5-nitrotoluene + NADH + O2
2-amino-5-nitrobenzylalcohol + aminonitrocresol + nitrite + NAD+
-
34.4% yield
-
?
2-amino-6-nitrotoluene + NADH + H+ + O2
2-amino-4-nitro-3-cresol + 3-amino-5-nitro-4-cresol + NAD+ + H2O
-
-
-
?
2-amino-6-nitrotoluene + NADH + H+ + O2
2-amino-4-nitro-3-cresol + 3-amino-5-nitro-4-cresol + NAD+ + H2O
-
-
-
?
2-amino-6-nitrotoluene + NADH + O2
2-amino-4-nitro-m-cresol + 3-amino-5-nitro-p-cresol + nitrite + NAD+
-
20% yield
-
?
2-amino-6-nitrotoluene + NADH + O2
2-amino-4-nitro-m-cresol + 3-amino-5-nitro-p-cresol + nitrite + NAD+
-
20% yield
-
?
2-methoxyphenol + NADH + O2
methoxyhydroquinone + NAD+ + H2O
-
-
-
-
?
2-methoxyphenol + NADH + O2
methoxyhydroquinone + NAD+ + H2O
-
-
-
-
?
2-nitrophenol + NADH + O2
nitrohydroquinone + 3-nitrocatechol + NAD+ + H2O
-
-
-
-
?
2-nitrophenol + NADH + O2
nitrohydroquinone + 3-nitrocatechol + NAD+ + H2O
-
-
-
-
?
2-nitrotoluene + NADH + H+ + O2
2-nitrobenzyl alcohol + NAD+ + ?
-
-
-
?
2-nitrotoluene + NADH + H+ + O2
2-nitrobenzyl alcohol + NAD+ + ?
-
-
-
?
2-nitrotoluene + NADH + H+ + O2
2-nitrobenzyl alcohol + NAD+ + ?
-
-
-
?
2-nitrotoluene + NADH + H+ + O2
2-nitrobenzyl alcohol + NAD+ + ?
-
-
-
?
2-nitrotoluene + NADH + H+ + O2
2-nitrobenzyl alcohol + NAD+ + H2O
-
-
-
?
2-nitrotoluene + NADH + H+ + O2
2-nitrobenzyl alcohol + NAD+ + H2O
-
-
-
?
2-nitrotoluene + NADH + O2
?
-
low activity
-
-
?
2-nitrotoluene + NADH + O2
?
-
low activity
-
-
?
3-amino-4-nitrotoluene + NADH + O2
3-amino-4-nitrobenzylalcohol + aminonitrocresol + nitrite + NAD+
-
9% yield
-
?
3-amino-4-nitrotoluene + NADH + O2
3-amino-4-nitrobenzylalcohol + aminonitrocresol + nitrite + NAD+
-
9% yield
-
?
3-methyl-4-nitrophenol + NADH + O2
?
-
-
-
-
?
3-methyl-4-nitrophenol + NADH + O2
?
-
-
-
-
?
3-nitrophenol + NADH + O2
4-nitrocatechol + NAD+ + H2O
-
-
-
-
?
3-nitrophenol + NADH + O2
4-nitrocatechol + NAD+ + H2O
-
-
-
-
?
3-nitrotoluene + NADH + H+ + O2
3-nitrobenzyl alcohol + NAD+ + ?
-
-
-
?
3-nitrotoluene + NADH + H+ + O2
3-nitrobenzyl alcohol + NAD+ + ?
-
-
-
?
3-nitrotoluene + NADH + O2
4-methylcatechol + nitrite + NAD+
-
-
formation of 0.06 nmol 4-methylcatechol per mg protein plus 0.03 nmol 3-nitrobenzyl alcohol
-
?
3-nitrotoluene + NADH + O2
4-methylcatechol + nitrite + NAD+
-
-
formation of 0.06 nmol 4-methylcatechol per mg protein plus 0.03 nmol 3-nitrobenzyl alcohol
-
?
4-nitrotoluene + NADH + H+ + O2
4-nitrobenzyl alcohol + NAD+ + ?
-
-
-
?
4-nitrotoluene + NADH + H+ + O2
4-nitrobenzyl alcohol + NAD+ + ?
-
-
-
?
indan + NADH + H+ + O2
1-indanol + ? + NAD+
-
no formation of 1,2-dihydroxyindan
-
?
indan + NADH + H+ + O2
1-indanol + ? + NAD+
-
no formation of 1,2-dihydroxyindan
-
?
indoline + NADH + H+ + O2
indigo + ? + NAD+
-
-
-
?
indoline + NADH + H+ + O2
indigo + ? + NAD+
-
-
-
?
m-cresol + NADH + H+ + O2
methylhydroquinone + nitrite + NAD+
-
no substrate for wild-type. Mutant V350F produces methylhydroquinone at 0.43 nmol/min/mg of protein
-
?
m-cresol + NADH + H+ + O2
methylhydroquinone + nitrite + NAD+
-
no substrate for wild-type. Mutant V350F produces methylhydroquinone at 0.43 nmol/min/mg of protein
-
?
m-methoxyphenol + NADH + H+ + O2
methoxyhydroquinone + nitrite + NAD+
-
no substrate for wild-type. Mutant V350F produces methoxyhydroquinone at 0.55 nmol/min/mg of protein
-
?
m-methoxyphenol + NADH + H+ + O2
methoxyhydroquinone + nitrite + NAD+
-
no substrate for wild-type. Mutant V350F produces methoxyhydroquinone at 0.55 nmol/min/mg of protein
-
?
m-nitrophenol + NADH + H+ + O2
4-nitrocatechol + nitrite + NAD+
-
no substrate for wild-type. Mutant V350F produces 4-nitrocatechol at 0.29 nmol/min/mg of protein
-
?
m-nitrophenol + NADH + H+ + O2
4-nitrocatechol + nitrite + NAD+
-
no substrate for wild-type. Mutant V350F produces 4-nitrocatechol at 0.29 nmol/min/mg of protein
-
?
naphthalene + NADH + H+ + O2
(+)-cis-(1R,2S)-dihydroxy-1,2-dihydronaphthalene + NAD+ + ?
-
-
-
?
naphthalene + NADH + H+ + O2
(+)-cis-(1R,2S)-dihydroxy-1,2-dihydronaphthalene + NAD+ + ?
-
-
-
?
naphthalene + NADH + H+ + O2
(1R,2S)-cis-1,2-dihydroxy-1,2-dihydronaphthalene + NAD+
-
-
-
?
naphthalene + NADH + H+ + O2
(1R,2S)-cis-1,2-dihydroxy-1,2-dihydronaphthalene + NAD+
-
mutants V350F and V350M exhibit 10fold enhanced activity towards naphthalene
-
?
naphthalene + NADH + H+ + O2
(1R,2S)-cis-1,2-dihydroxy-1,2-dihydronaphthalene + NAD+
-
mutants V350F and V350M exhibit 10fold enhanced activity towards naphthalene
-
?
naphthalene + NADH + H+ + O2
(1R,2S)-cis-1,2-dihydroxy-1,2-dihydronaphthalene + NAD+
-
-
-
?
naphthalene + O2
cis-1,2-dihydroxy-1,2-dihydronaphthalene
-
-
formation of 0.83 nmol 3-methylcatechol per mg protein
-
?
naphthalene + O2
cis-1,2-dihydroxy-1,2-dihydronaphthalene
-
product is formed in 92% enantiomeric excess
-
?
naphthalene + O2
cis-1,2-dihydroxy-1,2-dihydronaphthalene
-
-
formation of 0.83 nmol 3-methylcatechol per mg protein
-
?
naphthalene + O2
cis-1,2-dihydroxy-1,2-dihydronaphthalene
-
product is formed in 92% enantiomeric excess
-
?
additional information
?
-
-
no activity with 2-cresol, 3-methoxyphenol
-
-
?
additional information
?
-
-
no activity with 2-cresol, 3-methoxyphenol
-
-
?
additional information
?
-
no activity with 2,3-dinitrotoluene and 2,5-dinitrotoluene
-
-
?
additional information
?
-
-
no activity with 2,3-dinitrotoluene and 2,5-dinitrotoluene
-
-
?
additional information
?
-
-
2-amino-4-nitrotoluene is not a substrate
-
-
?
additional information
?
-
2-amino-4-nitrotoluene is not a substrate
-
-
?
additional information
?
-
-
no activity with anisole, carbazole, and nitrobenzene
-
-
?
additional information
?
-
no activity with anisole, carbazole, and nitrobenzene
-
-
?
additional information
?
-
-
no substrate: 2-amino-4-nitrotoluene
-
-
?
additional information
?
-
no substrate: 2-amino-4-nitrotoluene
-
-
?
additional information
?
-
-
no substrate: 2-nitrotoluene, 3-nitrotoluene
-
-
?
additional information
?
-
-
2-amino-4-nitrotoluene is not a substrate
-
-
?
additional information
?
-
2-amino-4-nitrotoluene is not a substrate
-
-
?
additional information
?
-
-
no activity with anisole, carbazole, and nitrobenzene
-
-
?
additional information
?
-
no activity with anisole, carbazole, and nitrobenzene
-
-
?
additional information
?
-
-
no substrate: 2-nitrotoluene, 3-nitrotoluene
-
-
?
additional information
?
-
-
no substrate: 2-amino-4-nitrotoluene
-
-
?
additional information
?
-
no substrate: 2-amino-4-nitrotoluene
-
-
?
additional information
?
-
no activity with 2,3-dinitrotoluene and 2,5-dinitrotoluene
-
-
?
additional information
?
-
-
no activity with 3-nitrotoluene, 4-nitrotoluene, catechol, 3-nitrocatechol, 3-methylcatechol, and 4-methylcatechol
-
-
?
additional information
?
-
-
no activity with 3-nitrotoluene, 4-nitrotoluene, catechol, 3-nitrocatechol, 3-methylcatechol, and 4-methylcatechol
-
-
?
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V350F
-
the mutant of the alpha subunit is inactive with 2,4-dinitrotoluene but shows significantly increased activity towards 2-nitrophenol (47times), 3-nitrophenol (34times), and 2-methoxyphenol (174times) as well as an expanded substrate range that now includes 3-methoxyphenol, o-cresol, and m-cresol (wild type enzyme has no detectable activity for these substrates). The mutant also exhibits 10fold enhanced activity towards naphthalene forming (1R,2S)-cis-1,2-dihydro-1,2-dihydroxynaphthalene
V350M
-
the mutant is inactive with 2,4-dinitrotoluene but displays increased activity towards 2-nitrophenol (20times) and 2-methoxyphenol (162times) as well as novel activity towards 2-cresol compared to the wild type enzyme. The mutant also exhibits 10fold enhanced activity towards naphthalene forming (1R,2S)-cis-1,2-dihydro-1,2-dihydroxynaphthalene
V350F
-
the mutant of the alpha subunit is inactive with 2,4-dinitrotoluene but shows significantly increased activity towards 2-nitrophenol (47times), 3-nitrophenol (34times), and 2-methoxyphenol (174times) as well as an expanded substrate range that now includes 3-methoxyphenol, o-cresol, and m-cresol (wild type enzyme has no detectable activity for these substrates). The mutant also exhibits 10fold enhanced activity towards naphthalene forming (1R,2S)-cis-1,2-dihydro-1,2-dihydroxynaphthalene
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V350M
-
the mutant is inactive with 2,4-dinitrotoluene but displays increased activity towards 2-nitrophenol (20times) and 2-methoxyphenol (162times) as well as novel activity towards 2-cresol compared to the wild type enzyme. The mutant also exhibits 10fold enhanced activity towards naphthalene forming (1R,2S)-cis-1,2-dihydro-1,2-dihydroxynaphthalene
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I204L
the mutant transforms both 2,6-dinitrotoluene and 2,4-dinitrotoluene 2fold faster than the wild type enzyme and exhibits activity with 2,5- and 2,3-dinitrotoluene
V350F
mutant displays significantly increased activity towards o-nitrophenol (47 times), m-nitrophenol (34 times), and o-methoxyphenol (174 times) as well as an expanded substrate range that now includes m-methoxyphenol, o-cresol, and m-cresol
V350M
mutant displays increased activity towards o-nitrophenol (20 times) and o-methoxyphenol (162 times) as well as novel activity towards o-cresol
I204L
-
the mutant transforms both 2,6-dinitrotoluene and 2,4-dinitrotoluene 2fold faster than the wild type enzyme and exhibits activity with 2,5- and 2,3-dinitrotoluene
-
V350F
-
mutant displays significantly increased activity towards o-nitrophenol (47 times), m-nitrophenol (34 times), and o-methoxyphenol (174 times) as well as an expanded substrate range that now includes m-methoxyphenol, o-cresol, and m-cresol
-
V350M
-
mutant displays increased activity towards o-nitrophenol (20 times) and o-methoxyphenol (162 times) as well as novel activity towards o-cresol
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I204Y
mutation in alpha subunit DntAc, results in two- to fourfold faster oxidization of the aminonitrotoluenes
I204Y
the mutant transforms both 2,6-dinitrotoluene 2,5fold faster than the wild type enzyme and exhibits activity with 2,5- and 2,3-dinitrotoluene
I204Y
the mutation results in 2-4fold faster oxidization of the aminonitrotoluenes compared to the wild type enzyme
I204Y
-
the mutation results in 2-4fold faster oxidization of the aminonitrotoluenes compared to the wild type enzyme
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I204Y
-
mutation in alpha subunit DntAc, results in two- to fourfold faster oxidization of the aminonitrotoluenes
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I204Y
-
the mutant transforms both 2,6-dinitrotoluene 2,5fold faster than the wild type enzyme and exhibits activity with 2,5- and 2,3-dinitrotoluene
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additional information
-
construction of hybrid dioxygenases with the genes encoding 2-nitrotoluene 2,3-dioxygenase from Pseudomonas sp. strain JS42 and 2,4-dinitrotoluene dioxygenase from Burkholderia sp. strain DNT. The C-terminal region of the large subunit of the oxygenase component is responsible for the enzyme specificity differences observed between 2-nitrotoluene 2,3-dioxygenase and 2,4-dinitrotoluene dioxygenase
additional information
-
engineering of hybrid dioxygenase enzymes coexpressing genes from naphthalene and 2,4-dinitrotoluene dioxygenases in Escherichia coli. In the active hybrids, replacement of small subunits affects the rate of product formation but has no effect on the substrate range, regiospecificity, or enantiomeric purity of oxidation products with the substrates tested. The small subunit of the oxygenase is essential for activity but does not play a major role in determining the specificity of these enzymes. Introduction of the small subunit of 2-nitrotolene synthase from Pseudomonas sp. strain JS42 leads to 30% reduction in product formation from naphthalene and dinitrotoluene
additional information
engineering of hybrid dioxygenase enzymes coexpressing genes from naphthalene and 2,4-dinitrotoluene dioxygenases in Escherichia coli. In the active hybrids, replacement of small subunits affects the rate of product formation but has no effect on the substrate range, regiospecificity, or enantiomeric purity of oxidation products with the substrates tested. The small subunit of the oxygenase is essential for activity but does not play a major role in determining the specificity of these enzymes. Introduction of the small subunit of 2-nitrotolene synthase from Pseudomonas sp. strain JS42 leads to 30% reduction in product formation from naphthalene and dinitrotoluene
additional information
-
construction of hybrid dioxygenases with the genes encoding 2-nitrotoluene 2,3-dioxygenase from Pseudomonas sp. strain JS42 and 2,4-dinitrotoluene dioxygenase from Burkholderia sp. strain DNT. The C-terminal region of the large subunit of the oxygenase component is responsible for the enzyme specificity differences observed between 2-nitrotoluene 2,3-dioxygenase and 2,4-dinitrotoluene dioxygenase
-
additional information
-
engineering of hybrid dioxygenase enzymes coexpressing genes from naphthalene and 2,4-dinitrotoluene dioxygenases in Escherichia coli. In the active hybrids, replacement of small subunits affects the rate of product formation but has no effect on the substrate range, regiospecificity, or enantiomeric purity of oxidation products with the substrates tested. The small subunit of the oxygenase is essential for activity but does not play a major role in determining the specificity of these enzymes. Introduction of the small subunit of 2-nitrotolene synthase from Pseudomonas sp. strain JS42 leads to 30% reduction in product formation from naphthalene and dinitrotoluene
-
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Spanggord, R.J.; Spain, J.C.; Nishino, S.F.; Mortelmans, K.E.
Biodegradation of 2,4-dinitrotoluene by a Pseudomonas sp.
Appl. Environ. Microbiol.
57
3200-3205
1991
Pseudomonas sp., Pseudomonas sp. DNT
brenda
Keenan, B.G.; Leungsakul, T.; Smets, B.F.; Wood, T.K.
Saturation mutagenesis of Burkholderia cepacia R34 2,4-dinitrotoluene dioxygenase at DntAc valine 350 for synthesizing nitrohydroquinone, methylhydroquinone, and methoxyhydroquinone
Appl. Environ. Microbiol.
70
3222-3231
2004
Burkholderia cepacia, Burkholderia sp. (Q5BM20 and Q6QUY4 and Q45695 and Q6QUY3), Burkholderia sp. DNT (Q5BM20 and Q6QUY4 and Q45695 and Q6QUY3), Burkholderia cepacia R34
brenda
Khleifat, K.M.
Characterization of 2,4-dinitrotoluene dioxygenase from recombinant Escherichia coli strain PFJS39: Its direct interaction with vitreoscilla hemoglobin
Bioremediat. J.
14
38-53
2010
Burkholderia sp., Burkholderia sp. DNT
-
brenda
Leungsakul, T.; Keenan, B.G.; Yin, H.; Smets, B.F.; Wood, T.K.
Saturation mutagenesis of 2,4-DNT dioxygenase of Burkholderia sp. strain DNT for enhanced dinitrotoluene degradation
Biotechnol. Bioeng.
92
416-426
2005
Burkholderia sp. (Q6QUY3), Burkholderia sp., Burkholderia sp. DNT (Q6QUY3)
brenda
Leungsakul T, L.T.; Keenan B.G, K.B.; Mori M.-A, M.M.; Morton M.D, M.M.; Stuart J.D, S.J.; Smets B.F, S.B.; Wood T.K, W.T.
Oxidation of aminonitrotoluenes by 2,4-DNT dioxygenase of Burkholderia sp. strain DNT
Biotechnol. Bioeng.
93
231-237
2006
Burkholderia sp., Burkholderia sp. (Q5BM20 and Q6QUY4 and Q45695 and Q6QUY3), Burkholderia sp. DNT, Burkholderia sp. DNT (Q5BM20 and Q6QUY4 and Q45695 and Q6QUY3)
brenda
Suen, W.C.; Spain, J.C.
Cloning and characterization of Pseudomonas sp. strain DNT genes for 2,4-dinitrotoluene degradation
J. Bacteriol.
175
1831-1837
1993
Pseudomonas sp., Pseudomonas sp. DNT
brenda
Suen, W.C.; Haigler, B.E.; Spain, J.C.
2,4-Dinitrotoluene dioxygenase from Burkholderia sp. strain DNT: similarity to naphthalene dioxygenase
J. Bacteriol.
178
4926-4934
1996
Burkholderia sp., Burkholderia sp. (Q5BM20 and Q6QUY4 and Q45695 and Q6QUY3), Burkholderia sp. DNT, Burkholderia sp. DNT (Q5BM20 and Q6QUY4 and Q45695 and Q6QUY3)
brenda
Parales, J.; Parales, R.; Resnick, S.; Gibson, D.
Enzyme specificity of 2-nitrotoluene 2,3-dioxygenase from Pseudomonas sp. strain JS42 is determined by the C-terminal region of the alpha-subunit of the oxygenase component
J. Bacteriol.
180
1194-1199
1998
Burkholderia sp., Burkholderia sp. DNT
brenda
Parales, R.E.; Emig, M.D.; Lynch, N.A.; Gibson, D.T.
Substrate specificities of hybrid naphthalene and 2,4-dinitrotoluene dioxygenase enzyme systems
J. Bacteriol.
180
2337-2344
1998
Burkholderia sp., Burkholderia sp. (Q5BM20 and Q6QUY4 and Q45695 and Q6QUY3), Burkholderia sp. DNT, Burkholderia sp. DNT (Q5BM20 and Q6QUY4 and Q45695 and Q6QUY3)
brenda
Lin, J.M.; Stark, B.C.; Webster, D.A.
Effects of Vitreoscilla hemoglobin on the 2,4-dinitrotoluene (2,4-DNT) dioxygenase activity of Burkholderia and on 2,4-DNT degradation in two-phase bioreactors
J. Ind. Microbiol. Biotechnol.
30
362-368
2003
Burkholderia sp., Burkholderia sp. DNT
brenda
Fish P.A, F.P.; Webster D.A, W.D.; Stark B.C, S.B.
Vitreoscilla hemoglobin enhances the first step in 2,4-dinitrotoluene degradation in vitro and at low aeration in vivo
J. Mol. Catal. B
9
75-82
2000
Burkholderia sp., Burkholderia sp. DNT
-
brenda
Kuece, P.; Coral, G.; Kantar, C.
Biodegradation of 2,4-dinitrotoluene (DNT) by Arthrobacter sp. K1 isolated from a crude oil contaminated soil
Ann. Microbiol.
65
467-476
2015
Microbacterium saccharophilum
-
brenda