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(2S)-5-hydroxy-2-aminovaleric acid + NAD+ + H2O
(2S)-2-amino-5-oxopentanoate + NADH
-
no activity with the (2R)-isomer
-
-
?
(2S,4S)-5-hydroxyleucine + NAD+ + H2O
(4S)-5-oxo-L-leucine + NADH
1,3-propanediol + 2 NAD+ + H2O
? + 2 NADH + 2 H+
1-decanol + 2 NAD+ + H2O
decanoic acid + 2 NADH + 2 H+
1-docosanol + NAD+ + H2O
behenic acid + NADH + H+
-
-
-
-
?
1-dodecanol + 2 NAD+ + H2O
dodecanoic acid + 2 NADH + 2 H+
1-eicosanol + 2 NAD+ + H2O
eicosanoic acid + 2 NADH + 2 H+
1-hexadecanol + 2 NAD+ + H2O
hexadecanoic acid + 2 NADH + 2 H+
1-octacosanol + 2 NAD+ + H2O
octacosanoic acid + 2 NADH + 2 H+
1-octadecanol + 2 NAD+ + H2O
octadecanoic acid + 2 NADH + 2 H+
1-octanol + 2 NAD+ + H2O
octanoic acid + 2 NADH + 2 H+
1-octanol + 2 NADP+ + H2O
octanoic acid + 2 NADPH + 2 H+
-
-
-
r
1-tetracosanol + 2 NAD+ + H2O
tetracosanoic acid + 2 NADH + 2 H+
1-tetracosanol + NAD+ + H2O
lignoceric acid + NADH + H+
-
-
-
-
?
1-tetradecanol + 2 NAD+ + H2O
tetradecanoic acid + 2 NADH + 2 H+
10,12-dihydroxyoctadecanoic acid + NAD+ + H2O
?
-
-
-
-
?
10,12-dihydroxyoctadecanoic acid + NADP+ + H2O
? + NADPH
-
the reaction is catalyzed by mutant enzyme D37S/V39S
-
-
?
10-hydroxyoctadecanoic acid + NAD+ + H2O
9-(nonanoyloxy)nonanoic acid + NADH
-
-
-
-
?
10-hydroxyoctadecanoic acid + NADP+ + H2O
9-(nonanoyloxy)nonanoic acid + NADPH
-
the reaction is catalyzed by mutant enzyme D37S/A38R/V39S/T15I
-
-
?
12-hydroxydodecanoic acid + NAD+ + H2O
? + NADH
-
-
-
-
?
12-hydroxyoctadecanoic acid + NAD+ + H2O
? + NADH
-
-
-
-
?
4-decanol + NAD+ + H2O
? + NADH
-
-
-
-
?
decanal + NAD+ + H+
decanoic acid + NADH
about 10% of the activity with 1-octanol
-
-
r
dodecanal + NAD+ + H+
dodecanoic acid + NADH
about 10% of the activity with 1-octanol
-
-
r
ethanol + NAD+ + H2O
ethanal + NADH
-
-
-
-
?
glycerol + 2 NAD+ + H2O
? + 2 NADH + 2 H+
about 45% of the activity with ethanol
-
-
r
hexadecanol + NAD+ + H2O
hexadecanal + NADH
-
-
-
-
?
isopropanol + 2 NAD+ + H2O
? + 2 NADH + 2 H+
about 15% of the activity with 1-octanol
-
-
r
long-chain alcohol + NAD+ + H2O
long-chain carboxylate + NADH
n-butanol + NAD+ + H2O
n-butanal + NADH
n-decanol + NAD+ + H2O
n-decanal + NADH
-
-
-
-
r
n-heptanol + NAD+ + H2O
n-heptanal + NADH
-
-
-
-
?
n-hexanol + NAD+ + H2O
n-hexanal + NADH
n-nonanol + NAD+ + H2O
n-nonanal + NADH
-
-
-
-
?
n-octanol + NAD+ + H2O
n-octanal + NADH
-
-
-
-
?
n-pentanol + NAD+ + H2O
n-pentanal + NADH
n-propanol + NAD+ + H2O
n-propanal + NADH
octanal + NAD+ + H2O
octanoic acid + NADH + H+
additional information
?
-
(2S,4S)-5-hydroxyleucine + NAD+ + H2O
(4S)-5-oxo-L-leucine + NADH
-
-
-
-
r
(2S,4S)-5-hydroxyleucine + NAD+ + H2O
(4S)-5-oxo-L-leucine + NADH
-
enzyme reaction is part of the biosynthesis of (2S,4S)-4-methylproline
-
-
r
1,3-propanediol + 2 NAD+ + H2O
? + 2 NADH + 2 H+
about 45% of the activity with ethanol
-
-
r
1,3-propanediol + 2 NAD+ + H2O
? + 2 NADH + 2 H+
about 5% of the activity with 1-octanol
-
-
r
1,3-propanediol + 2 NAD+ + H2O
? + 2 NADH + 2 H+
about 45% of the activity with ethanol
-
-
r
1,3-propanediol + 2 NAD+ + H2O
? + 2 NADH + 2 H+
about 5% of the activity with 1-octanol
-
-
r
1-decanol + 2 NAD+ + H2O
decanoic acid + 2 NADH + 2 H+
about 20% of the activity with ethanol
-
-
r
1-decanol + 2 NAD+ + H2O
decanoic acid + 2 NADH + 2 H+
about 50% of the activity with 1-octanol
-
-
r
1-decanol + 2 NAD+ + H2O
decanoic acid + 2 NADH + 2 H+
about 20% of the activity with ethanol
-
-
r
1-decanol + 2 NAD+ + H2O
decanoic acid + 2 NADH + 2 H+
about 50% of the activity with 1-octanol
-
-
r
1-dodecanol + 2 NAD+ + H2O
dodecanoic acid + 2 NADH + 2 H+
about 17% of the activity with ethanol
-
-
r
1-dodecanol + 2 NAD+ + H2O
dodecanoic acid + 2 NADH + 2 H+
about 30% of the activity with 1-octanol
-
-
r
1-dodecanol + 2 NAD+ + H2O
dodecanoic acid + 2 NADH + 2 H+
about 17% of the activity with ethanol
-
-
r
1-dodecanol + 2 NAD+ + H2O
dodecanoic acid + 2 NADH + 2 H+
about 30% of the activity with 1-octanol
-
-
r
1-eicosanol + 2 NAD+ + H2O
eicosanoic acid + 2 NADH + 2 H+
about 25% of the activity with 1-octanol
-
-
r
1-eicosanol + 2 NAD+ + H2O
eicosanoic acid + 2 NADH + 2 H+
about 3% of the activity with ethanol
-
-
r
1-eicosanol + 2 NAD+ + H2O
eicosanoic acid + 2 NADH + 2 H+
about 3% of the activity with ethanol
-
-
r
1-eicosanol + 2 NAD+ + H2O
eicosanoic acid + 2 NADH + 2 H+
about 25% of the activity with 1-octanol
-
-
r
1-hexadecanol + 2 NAD+ + H2O
hexadecanoic acid + 2 NADH + 2 H+
about 10% of the activity with ethanol
-
-
r
1-hexadecanol + 2 NAD+ + H2O
hexadecanoic acid + 2 NADH + 2 H+
about 25% of the activity with 1-octanol
-
-
r
1-octacosanol + 2 NAD+ + H2O
octacosanoic acid + 2 NADH + 2 H+
about 40% of the activity with ethanol
-
-
r
1-octacosanol + 2 NAD+ + H2O
octacosanoic acid + 2 NADH + 2 H+
about 5% of the activity with 1-octanol
-
-
r
1-octadecanol + 2 NAD+ + H2O
octadecanoic acid + 2 NADH + 2 H+
about 15% of the activity with 1-octanol
-
-
r
1-octadecanol + 2 NAD+ + H2O
octadecanoic acid + 2 NADH + 2 H+
about 5% of the activity with ethanol
-
-
r
1-octanol + 2 NAD+ + H2O
octanoic acid + 2 NADH + 2 H+
-
-
-
-
r
1-octanol + 2 NAD+ + H2O
octanoic acid + 2 NADH + 2 H+
-
-
-
r
1-tetracosanol + 2 NAD+ + H2O
tetracosanoic acid + 2 NADH + 2 H+
about 10% of the activity with 1-octanol
-
-
r
1-tetracosanol + 2 NAD+ + H2O
tetracosanoic acid + 2 NADH + 2 H+
about 30% of the activity with ethanol
-
-
r
1-tetradecanol + 2 NAD+ + H2O
tetradecanoic acid + 2 NADH + 2 H+
about 15% of the activity with ethanol
-
-
r
1-tetradecanol + 2 NAD+ + H2O
tetradecanoic acid + 2 NADH + 2 H+
about 30% of the activity with 1-octanol
-
-
r
long-chain alcohol + NAD+ + H2O
long-chain carboxylate + NADH
-
enzyme also uses formaldehyde and short chain aliphatic alcohols
-
-
?
long-chain alcohol + NAD+ + H2O
long-chain carboxylate + NADH
-
C17-C-19 alcohols do not serve as a good substrate
-
-
?
long-chain alcohol + NAD+ + H2O
long-chain carboxylate + NADH
-
maximum activity on C14 alcohol
-
-
?
long-chain alcohol + NAD+ + H2O
long-chain carboxylate + NADH
-
C8-C16
-
-
?
long-chain alcohol + NAD+ + H2O
long-chain carboxylate + NADH
-
peroxisomal enzyme is participating in fatty acid degradation
-
-
?
long-chain alcohol + NAD+ + H2O
long-chain carboxylate + NADH
-
microsomal and mitochondrial enzyme may have an indispensable role in lipid biosynthesis
-
-
?
long-chain alcohol + NAD+ + H2O
long-chain carboxylate + NADH
-
long chain n-alcanols up to C12
-
-
r
long-chain alcohol + NAD+ + H2O
long-chain carboxylate + NADH
-
maximal activity with decanol
-
-
?
long-chain alcohol + NAD+ + H2O
long-chain carboxylate + NADH
-
C10-C18
-
-
?
long-chain alcohol + NAD+ + H2O
long-chain carboxylate + NADH
-
enzyme may play an important role in regulating the cellular levels of ether-linked lipids
-
-
?
long-chain alcohol + NAD+ + H2O
long-chain carboxylate + NADH
-
C6-C18 alcohols
-
-
?
long-chain alcohol + NAD+ + H2O
long-chain carboxylate + NADH
-
maximal activity with decanol
-
-
?
long-chain alcohol + NAD+ + H2O
long-chain carboxylate + NADH
-
C17-C-19 alcohols do not serve as a good substrate
-
-
?
long-chain alcohol + NAD+ + H2O
long-chain carboxylate + NADH
-
peroxisomal enzyme is participating in fatty acid degradation
-
-
?
long-chain alcohol + NAD+ + H2O
long-chain carboxylate + NADH
-
microsomal and mitochondrial enzyme may have an indispensable role in lipid biosynthesis
-
-
?
n-butanol + NAD+ + H2O
n-butanal + NADH
-
-
-
-
?
n-butanol + NAD+ + H2O
n-butanal + NADH
-
-
-
-
?
n-hexanol + NAD+ + H2O
n-hexanal + NADH
-
-
-
-
?
n-hexanol + NAD+ + H2O
n-hexanal + NADH
-
-
-
-
?
n-pentanol + NAD+ + H2O
n-pentanal + NADH
-
-
-
-
?
n-pentanol + NAD+ + H2O
n-pentanal + NADH
-
-
-
-
?
n-propanol + NAD+ + H2O
n-propanal + NADH
-
-
-
-
?
n-propanol + NAD+ + H2O
n-propanal + NADH
-
-
-
-
?
octanal + NAD+ + H2O
octanoic acid + NADH + H+
-
-
-
r
octanal + NAD+ + H2O
octanoic acid + NADH + H+
-
-
-
r
additional information
?
-
substrates are a broad range of alkyl alcohols from ethanol to 1-triacontanol
-
-
?
additional information
?
-
substrates are a broad range of alkyl alcohols from ethanol to 1-triacontanol
-
-
?
additional information
?
-
substrates are a broad range of alkyl alcohols from ethanol to 1-triacontanol
-
-
?
additional information
?
-
substrates are a broad range of alkyl alcohols from ethanol to 1-triacontanol
-
-
?
additional information
?
-
-
the wild type enzyme shows no activity with NADP+
-
-
-
additional information
?
-
-
stereospecific abstraction of the 5pro-S hydrogen
-
-
?
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67.2
1-Octanol
cosubstrate NADP+, pH 8.0, 60°C
0.0021 - 0.012
10,12-dihydroxyoctadecanoic acid
-
0.0014 - 0.013
10-hydroxyoctadecanoic acid
0.00061 - 0.0015
12-hydroxyoctadecanoic acid
26.7
NAD+
cosubstrate 1-octanol, pH 8.0, 60°C
117.9
NADP+
cosubstrate 1-octanol, pH 8.0, 60°C
5724
NADPH
cosubstrate octanal, pH 8.0, 60°C
450.8
octanal
cosubstrate NADPH, pH 8.0, 60°C
0.0021
10,12-dihydroxyoctadecanoic acid
-
mutant enzyme D37S/A38R/V39S/T15I, with NAD+ as cosubstrate, pH and temperature not specified in the publication
-
0.0035
10,12-dihydroxyoctadecanoic acid
-
wild type enzyme, with NAD+ as cosubstrate, pH and temperature not specified in the publication
-
0.012
10,12-dihydroxyoctadecanoic acid
-
mutant enzyme D37S/V39S, with NAD+ as cosubstrate, pH and temperature not specified in the publication
-
0.0014
10-hydroxyoctadecanoic acid
-
mutant enzyme D37S/V39S, with NAD+ as cosubstrate, pH and temperature not specified in the publication
0.0014
10-hydroxyoctadecanoic acid
-
wild type enzyme, with NAD+ as cosubstrate, pH and temperature not specified in the publication
0.013
10-hydroxyoctadecanoic acid
-
mutant enzyme D37S/A38R/V39S/T15I, with NAD+ as cosubstrate, pH and temperature not specified in the publication
0.00061
12-hydroxyoctadecanoic acid
-
wild type enzyme, with NAD+ as cosubstrate, pH and temperature not specified in the publication
0.0015
12-hydroxyoctadecanoic acid
-
mutant enzyme D37S/V39S, with NAD+ as cosubstrate, pH and temperature not specified in the publication
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Van Ophem, P.W.; Van Beeumen, J.; Duine, J.A.
NAD-linked, factor-dependent formaldehyde dehydrogenase or trimeric, zinc-containing, long-chain alcohol dehydrogenase from Amycolatopsis methanolica
Eur. J. Biochem.
206
511-518
1992
Amycolatopsis methanolica
brenda
Lee, T.C.
Characterization of fatty alcohol:NAD+ oxidoreductase from rat liver
J. Biol. Chem.
254
2892-2896
1979
Rattus norvegicus
brenda
Ueda, M.; Tanaka, A.
Long-chain alcohol dehydrogenase of Candida yeast
Methods Enzymol.
188
171-175
1990
Yarrowia lipolytica, Candida tropicalis
brenda
Yamada, T.; Nawa, H.; Kawamoto, S.; Tanaka, A.; Fukui, S.
Subcellular localization of long-chain alcohol dehydrogenase and aldehyde dehydrogenase in n-alkane-grown Candida tropicalis
Arch. Microbiol.
128
145-151
1980
Candida tropicalis
brenda
Ribas de Pouplana, L.; Atrian, S.; Gonzalez-Duarte, R.; Fothergill-Gilmore, L.A.; Kelly, S.M.; Price, N.C.
Structural properties of long- and short-chain alcohol dehydrogenases. Contribution of NAD+ to stability
Biochem. J.
276
433-438
1991
Equus caballus
-
brenda
Nagashima, H.; Inoue, J.; Sasaki, E.; Yamamoto, S.; Sasaki, Y.; Yamauchi-Inomata, Y.; Harayama, S.
Long-chain n-alkanol dehydrogenase from Pseudomonas putida
J. Ferment. Bioeng.
82
328-333
1996
Pseudomonas putida
-
brenda
Eklund, H.; Nordstrom, B.; Zeppezauer, E.; Soderlund, G.; Ohlsson, I.; Boiwe, T.; Soderberg, B.O.; Tapia, O.; Branden, C.I.; Akeson, A.
Three-dimensional structure of horse liver alcohol dehydrogenase at 2-4 A resolution
J. Mol. Biol.
102
27-59
1976
Equus caballus
brenda
Luesch, H.; Hoffmann, D.; Hevel, J.M.; Becker, J.E.; Golakoti, T.; Moore, R.E.
Biosynthesis of 4-methylproline in cyanobacteria: cloning of nosE and nosF genes and biochemical characterization of the encoded dehydrogenase and reductase activities
J. Org. Chem.
68
83-91
2003
Nostoc sp.
brenda
Liu, X.; Dong, Y.; Zhang, J.; Zhang, A.; Wang, L.; Feng, L.
Two novel metal-independent long-chain alkyl alcohol dehydrogenases from Geobacillus thermodenitrificans NG80-2
Microbiology
155
2078-2085
2009
Geobacillus thermodenitrificans (A4IP64), Geobacillus thermodenitrificans (A4ISB9), Geobacillus thermodenitrificans NG80-2 (A4IP64), Geobacillus thermodenitrificans NG80-2 (A4ISB9)
brenda
Ottone, C.; Bernal, C.; Serna, N.; Illanes, A.; Wilson, L.
Enhanced long-chain fatty alcohol oxidation by immobilization of alcohol dehydrogenase from S. cerevisiae
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102
237-247
2018
Saccharomyces cerevisiae
brenda
Seo, E.J.; Kim, H.J.; Kim, M.J.; Kim, J.S.; Park, J.B.
Cofactor specificity engineering of a long-chain secondary alcohol dehydrogenase from Micrococcus luteus for redox-neutral biotransformation of fatty acids
Chem. Commun. (Camb.)
55
14462-14465
2019
Micrococcus luteus
brenda